Zbigniew Darzynkiewicz, M.D., Ph.D.


 

 About Zbigniew 
  • Biography
  • Curriculum Vitae
  • Publications
  • Books
  • Chapters
  • Presentations
  • Research Accomplishments
  • US Patents
  • Most Cited Papers
  • Interactions with Poland
     

     Essential Cytometry Methods 
  • Preface
  • Table of Contents
     

     Cytometry 4th Edition 
  • Preface
  • Table of Contents

      LIST OF PUBLICATIONS:

    PUBLICATIONS, FULL PAPERS, PEER REVIEWED

    1963

    1. Darzynkiewicz Z. The use of vinyl superchloride in histologic technique. Folia Morphologica, (Warsaw) 1963; 14:97-100.

    2. Ostrowski K, Barnard EA, Stocka Z, Darzynkiewicz Z. Autoradiographic methods in enzyme cytochemistry. I. Localization of acetylcholinesterase activity using a 3H labeled irreversible inhibitor. Exp Cell Res 1963; 31:89-99.

    3. Darzynkiewicz Z. Dynamics of incorporation of 35S sulphate into limb anlagen of chick embryos. Folia Histochem Cytochem 1963; 1:269-283.

    4. Ostrowski K, Darzynkiewicz Z, Sawicki W, Stocka Z. The possibilities of application of the new model of an interference microscope (MPI) in biologic research. Folia Histochem Cytochem 1963; 1:553-562.

    5. Sawicki W, Darzynkiewicz Z. The application of polyvinyl chloride films in autoradiography. Folia Histochem Cytochem 1963; 1:283-288.

    1964

    6. Ostrowski K, Darzynkiewicz Z, Sawicki W, Stocka Z. Dry mass of epithelial cells from the vagina of mice in the course of oestrus cycle measured by interference microscopy. Acta Biochim Polonica 1964; 11:99-105.

    7. Ostrowski K, Barnard EA, Darzynkiewicz Z, Rymaszewska D. Autoradiographic methods in enzyme cytochemistry. III. Measurements of esterases in the cells of mouse kidney. Exp Cell Res 1964; 36:43-52.

    1965

    8. Darzynkiewicz Z, Jurukova Z, Wieckowski J. Interferometric measurements of the alkaline phosphatase activity in epithelial cells from the vagina of mice in the course of keratinization process. Bull Acad Pol Sci 1965; 13:201-205.

    9. Wieckowski J, Darzynkiewicz Z. Studies on the kinetics of alkaline phosphatase reaction in Ehrlich ascites tumor cells with the use of interference microscopy. Experientia 1965; 21:387-388.

    10. Darzynkiewicz Z, Wieckowski J. Use of interference microscopy for quantitative estimation of alkaline phosphatase activity in Ehrlich ascites tumor cells. Folia Histochem Cytochem 1965; 3:275-289.

    11. Zaleski M, Rymaszewska T, Darzynkiewicz Z, Wlodarski K. Autoradiographic studies of cells from lymph nodes draining the tissue graft area. Bull Acad Pol Sci 1965; 13:43-47.

    12. Kawiak J, Moskalewski S, Darzynkiewicz Z. Isolation of chondrocytes from nasal cartilage of calf. Exp Cell Res 1965; 39:59-68.

    13. Darzynkiewicz Z, Krassowski T, Skopinska E. Stimulating effect of phytohemagglutinin upon "rapidly labelled" ribonucleic acid synthesis in human lymphocytes. Bull Acad Pol Sci 1965; 13:433-437.

    14. Darzynkiewicz. Z, Krassowski T, Skopinska E. Effect of phytohemagglutinin on synthesis of "rapidly labeled" ribonucleic acid in human lymphocytes. Nature 1965; 207:1402-1403.

    1966

    15. Darzynkiewicz Z, Rogers AW, Barnard EA. Measurements of esterases in rat megakaryocytes by the labeled inhibitor method. J Histochem Cytochem 1966; 14:807-810.

    16. Jablonska S, Langner A, Stachow A, Darzynkiewicz Z. L'importance generale d'acantholyse. In: "Pemphigus, occupational dermatoses due to chemical sensitization." Budapest, pp. 362-365, 1966.

    17. Darzynkiewicz Z, Rogers AW, Barnard EA, Wang DH, Werkheiser WC. Autoradiography with tritiated methotrexate and the cellular distribution of folate reductase. Science 1966; 151:1528-1530.

    18. Darzynkiewicz Z, Rogers AW, Barnard EA. Quantitative analysis of the esterases in rat megakaryocytes by radioisotope cytochemical technique. J Histochem Cytochem, 1966; 14:379-384.

    19. Darzynkiewicz Z, Rogers AW, Barnard EA. The number of acetyl-cholinesterase molecules in the rat megakaryocyte. J Histochem Cytochem 1966; 14:915-922..

    20. Rogers AW, Darzynkiewicz Z, Barnard EA, Salpeter MM. Number and location of acetylcholinesterase molecules at motor endplates of the mouse. Nature 1966; 210:1003-1006.

    1967

    21. Darzynkiewicz Z, Barnard EA. Specific proteases of the rat mast cell. Nature 1967; 213:1198-1202.

    22. Budd GC, Darzynkiewicz Z, Barnard EA. Intracellular localization of specific proteases in rat mast cells. Nature 1967; 213:1202-1203.

    23. Darzynkiewicz Z, Dokov V, Pienkowski M. Dry mass of lymphocytes during transformation after stimulation by phytohemagglutinin. Nature 1967; 214:1265-1266.

    24. Darzynkiewicz Z, Langner A, Pienkowski, M. Effect of penicillin on RNA synthesis in lymphocytes of penicillin-sensitized individuals. Experientia 1967; 23:840-841.

    25. Darzynkiewicz Z, Komender J. Autoradiographic detection of sodium-24 ions in single cells. J Histochem Cytochem 1967; 15:605-607.

    1968

    26. Langner A, Jablonska S, Darzynkiewicz. Autoradiographic studies on DNA synthesis by epidermal cells in epidermodysplasia verruciformis. Acta Dermatol Venerol (Stockholm) 1968; 48:501-510.

    27. Darzynkiewicz Z. Cell Biology, chapter in: The Human Being ("Czlowiek"). Darzynkiewicz et al. (eds.), Wiedza Powszechna, Warsaw, pp. 1-98, 1968 (in Polish).

    1969

    28. Darzynkiewicz Z, Pienkowski M. Effect of prednisolone on RNA synthesis in human lymphocytes stimulated by phytohemagglutinin. Exp Cell Res 1969; 54:289-292.

    29. Darzynkiewicz Z, Pienkowski M. Autoradiographic studies on the stability of ribonucleic acid in phytohemagglutinin-stimulated lymphocytes. Exp Cell Res 1969; 55:118-120.

    30. Rogers AW, Darzynkiewicz Z, Salpeter MM, Ostrowski K, Barnard EA. Quantitative studies on enzymes in structures of striated muscles by labeled inhibitor methods. J Cell Biol 1069; 41:665-689.

    31. Darzynkiewicz Z, Bolund L, Ringertz NR. Actinomycin D binding of normal and phytohemagglutinin stimulated lymphocytes. Exp Cell Res 1969; 55:120-126.

    32. Bolund L, Darzynkiewicz Z, Ringertz NR. Growth of hen erythrocytes nuclei undergoing reactivation in heterokaryons. Exp Cell Res 1969; 56:406-410.

    33. Ringertz NR, Darzynkiewicz Z, Bolund L. Actinomycin D binding properties of stimulated human lymphocytes. Exp Cell Res 1969; 56:411-417.

    34. Darzynkiewicz Z, Bolund L, Ringertz NR. Nucleo-protein changes and initiation of RNA synthesis in PHA stimulated lymphocytes. Exp Cell Res 1969; 56:418-424.

    35. Darzynkiewicz Z, Gledhill BL, Ringertz NR. Changes in deoxyribo-nucleoprotein during spermiogenesis in the bull. 3H-Actinomycin D binding. Exp Cell Res 1969; 58:435-438.

    36. Langner A, Jablonska S, Darzynkiewicz . Studies on the origin of the mucin in mucinosis follicularis. Acta Dermatol Venerol (Stockholm) 1969; 49:76-81.

    1970

    37. Ringertz NR, Gledhill BL, Darzynkiewicz Z. Changes in deoxyri-bonucleoprotein during spermiogenesis in the bull. II. Sensitivity of DNA to heat denaturation. Exp Cell Res 1970; 62:204-218.

    38. Bolund L, Darzynkiewicz Z, Ringertz NR. Cell concentration and the staining properties of nuclear deoxyribonucleoprotein. Exp Cell Res 1970; 62:76-83.

    39. Ringertz NR, Bolund L, Darzynkiewicz Z. Acridine orange binding of intracellular nucleic acids in fixed cells in relation to cell growth. Exp Cell Res 1970; 63:233-238

    40. Darzynkiewicz Z. Histochemistry and Cytochemistry, chapter in: Textbook of Cell Biology. Ostrowski K et al., (eds.) School of Medicine in Warsaw (Publ.), pp. 115-160, 1970 (in Polish).

    41. Darzynkiewicz Z. The Genetic Code, chapter in: Textbook of Cell Biology. Ostrowski K et al., (eds) School of Medicine in Warsaw (Publ.) pp. 195-265, 1970 (in Polish).

    1971

    42. Darzynkiewicz, Z., and Jacobson, B.: HEPES-buffered media in lymphocyte cultures. Proc. Soc. Exp. Biol. Med., 136:387-393, 1971.

    43. Gledhill BL, Darzynkiewicz Z, Ringertz NR. Changes in deoxyribonucleoprotein during spermiogenesis in the bull. Increased 3H-Actinomycin D binding to nuclear chromatin of morphologically abnormal spermatozoa. J Reprod Fertil 1971; 26:25-38.

    44. Darzynkiewicz Z, Balazs EA. Effect of connective tissue intercellular matrix on lymphocyte stimulation. I. Suppression of lymphocyte stimulation by hyaluronic acid. Exp Cell Res 1971; 66:113-123.

    45. Darzynkiewicz Z, Andersson J. Effect of prednisolone on thymus lymphocytes. I. Autoradiographic studies on 3H-Actinomycin D binding and 3H-uridine incorporation. Exp Cell Res 1971; 67:39-48.

    46. Darzynkiewicz Z, Jacobson B. Effect of prednisolone on thymus lymphocytes. II. Studies on 3H-Actinomycin D binding and RNA polymerase activity of isolated nuclei. Exp Cell Res 1971, 67:49-60.

    47. Darzynkiewicz Z, Pienkowski M. Studies on lymphocyte stimulation. Acta Physiol Pol (Warsaw) 1971; 22:791-798 (in Polish).

    48. Darzynkiewicz Z. Radiation-induced DNA synthesis in nuclei of hen erythrocytes reactivated in heterokaryons. Exp Cell Res 1971; 69:477-481.

    49. Darzynkiewicz Z. Radiation-induced DNA synthesis in normal and stimulated human lymphocytes. Exp Cell Res 1971; 69:356-360.

    1972

    50. Ritzen M, Carlsson SA, Darzynkiewicz Z. Cytochemical evidence of nucleoprotein changes in rat adrenal cortex following hypophysectomy or dexamethasone suppression. Exp Cell Res 1972; 70:46-50.

    51. Penn A, Gledhill B, Darzynkiewicz Z. Modification of the gelatin substrate procedure for demonstration of acrosomal proteolytic activity. J Histochem Cytochem 1972; 20:499-506.

    52. Darzynkiewicz Z, Chelmicka-Szorc E. Unscheduled DNA synthesis in hen erythrocyte nuclei reactivated in heterokaryons. Exp Cell Res 1972; 74:131-139.

    53. Darzynkiewicz Z, Chelmicka-Szorc E, Arnason BGW. UV-induced DNA synthesis in nuclei of Xeroderma Pigmento sum cells in heterokaryons. Exp Cell Res 1972; 74:602-608.

    54. Anderson J, Darzynkiewicz Z. Nucleoprotein changes and uridine incorporation in rat thymus lymphocytes. I. Cellular characterization of age-dependent thymus involution. Exp Cell Res 1972; 75:410-415.

    55. Anderson J, Darzynkiewicz Z. Nucleoprotein changes and uridine incorporation in rat thymus lymphocytes. II. Effect of treatment with anti-thymocyte serum in vitro. Exp Cell Res 1972; 75:417-423.

    56. Darzynkiewicz Z, Balazs EA. Effect of Connective Tissue Intercellular Matrix on Lymphocyte Stimulation. Chapter in: Experimental Control of Mitosis, Vol. II. McCormick et al., (Eds), MSS Information Corp., New York, pp. 160-170, 1972.

    1973

    57. Chakrabarti B, Darzynkiewicz Z. Cortisol-induced changes in UV absorption spectra of isolated nuclei from rat liver and thymus. Biochem Biophys Res Comm 1973; 50:164-171.

    58. Gledhill BL, Darzynkiewicz Z. Unscheduled DNA synthesis during mammalian spermatogenesis in response to U.V. irradiation. J Exp Zool 1973; 183:375-382.

    59. Darzynkiewicz Z. Detection of DNA polymerase activity in fixed cells. Exp Cell Res 1973; 80:483-486.

    60. Darzynkiewicz Z, Gledhill BL. Uptake of tritium labeled adenosine triphosphate by the cytoplasm of fixed interstitial cells from rat testes. J Histochem Cytochem 1973; 21:1035-1040.

    61. Balazs EA, Darzynkiewicz Z. The effect of hyaluronic acid on fibroblasts, mononuclear phagocytes and lymphocytes Chapter in: Biology of the Fibroblast, Papers of the Symposium held in Turku, Finland, 1972; Kulonen E, and Pikkarainen J, (Eds), Academic Press, London, pp. 237-252, 1973.

    1974

    62. Darzynkiewicz Z, Arnason BGW. Suppression of RNA synthesis in lymphocytes by inhibitors of proteolytic enzymes. Exp Cell Res 1974; 85:95-104.

    63. Darzynkiewicz Z, Chelmicka-Szorc E, Arnason BGW. Suppressive effect of protease inhibitors on heterokaryons containing chick erythrocyte nuclei. Exp Cell Res 1974; 87:333-345.

    64. Darzynkiewicz Z, Chelmicka-Szorc E, Arnason BGW. Chick erythrocyte nucleus reactivation in heterokaryons: Suppression by inhibitors of proteolytic enzymes. Proc Natl Acad Sci USA 1974; 71:644-647.

    65. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Thermally-induced changes in chromatin of isolated nuclei and of intact cells as revealed by acridine orange staining. Biochem Biophys Res Comm 1974; 59:392-399.

    1975

    66. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry. Exp Cell Res 1975; 90:411-428; (Reprinted in Yearbook of Cancer, 1975).

    67. Braunstein JD, Melamed MR, Darzynkiewicz Z, Traganos F, Sharpless T, Good RA.Quantitation of transformed lymphocytes by cytofluorometry. I. Phytohemagglutinin response. Clin Immunol Immunopathol 1975; 4:209-215.

    68. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Acridine orange staining and automated cytofluorometry in studies of conformation changes in nuclear chromatin Chapter in: Pulse Cytophotometry, Haanen CAM et al., (Eds), European Press-Medicon, Ghent, Belgium, pp. 88-102, 1975.

    69. Traganos F, Darzynkiewicz Z, Sharpless T, Melamed MR. Denaturation of deoxyribonucleic acid in situ: Effect of formaldehyde. J Histochem Cytochem 1975; 23:431-438.

    70. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Conformation of RNA in situ as studied by acridine orange staining and automated cytofluorometry. Exp Cell Res 1975; 95:143-153.

    71. Sharpless T, Traganos F, Darzynkiewicz Z, Melamed MR. Flow cytofluoro-metry: Discrimination between single cells and cell aggregates by direct size measure-ments. Acta Cytol 1975; 19:577-581.

    1976

    72. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. DNA denaturation in situ. Effect of divalent cations and alcohols. J Cell Biol 1976; 68:1-10.

    73. Traganos F, Darzynkiewicz Z, Sharpless T, Melamed MR. Cytofluorometric studies on conformation of nucleic acids in situ. I. Restriction of acridine orange binding by chromatin proteins. J Histochem Cytochem 1976; 24:40-48.

    74. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Cytofluorometric studies on conformation of nucleic acids in situ. II. Denaturation of DNA. J Histochem Cytochem 1976; 24:49-58.

    75. Darzynkiewicz Z, Traganos F, Sharpless T, Friend C, Melamed MR. Nuclear chromatin changes during erythroid differentiation of Friend-virus induced leukemic cells. Exp Cell Res 1976; 99:301-309.

    76. Melamed MR, Traganos F, Sharpless T, Darzynkiewicz Z. Urinary cytology automation: Preliminary studies with acridine orange staining and flow-through cytofluo-rometry. Invest Urol 1976; 13:331-338.

    77. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Effect of 0.25 M sodium chloride treatment on DNA denaturation in situ in thymus lymphocytes. Exp Cell Res 1976; 100:393-396.

    78. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Lymphocyte stimulation: A rapid multiparameter analysis. Proc Natl Acad Sci USA 1976; 73:2881-2884.

    79. Darzynkiewicz Z. Potential of fluorescent molecular probes as markers for instrumental evaluation of cells in the female reproduction tract. Chapter in: The Automation of Uterine Cancer Cytology, Wied GI, Bahr GF, Bartels PH (Eds), Tutorials of Cytology, Chicago, Ill., pp. 364-369, 1976.

    1977

    80. Traganos F, Darzynkiewicz Z, Sharpless T, Melamed MR. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem 1977; 25:46-56.

    81. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Different sensitivity of DNA in situ in interphase and metaphase chromatin to heat denaturation. J Cell Biol 1977; 73:128-138.

    82. Melamed MR, Darzynkiewicz Z, Traganos F, Sharpless T. Nucleic acid content and nuclear chromatin structure of human bladder cell lines as studied by flow cytofluorometry. Cancer Res 1977; 37:1227-1232.

    83. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Recognition of cells in mitosis by flow cytofluorometry. J Histochem Cytochem 1977; 25:875-880.

    84. Traganos F, Gorski AJ, Darzynkiewicz Z, Sharpless T, Melamed MR. Rapid multiparameter analysis of cell stimulation in mixed lymphocyte culture (MLC) reaction. J Histochem Cytochem 1977; 25:881-887.

    85. Melamed MR, Darzynkiewicz Z, Sharpless T, Traganos F. Cytology automation by flow cytometry. Cancer Res 1977; 37:2806-2812.

    86. Traganos F, Darzynkiewicz Z, Sharpless T, Melamed MR. Nucleic acid content of cell cycle distribution of five human bladder cell lines analyzed by flow cytofluorometry. Int J Cancer 1977; 20:30-36.

    87. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Cell cycle related changes in nuclear chromatin of stimulated lymphocytes as measured by flow cytometry. Cancer Res 1977; 37:4635-4640.

    88. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Interphase and metaphase chromatin: Different stainability of DNA with acridine orange. Exp Cell Res 1977; 110:201-214.

    1978

    89. Kurland J, Traganos F, Darzynkiewicz Z, Moore M. Macrophage mediated cytostasis of neoplastic hematopoietic cells. Cytofluorometric analysis of cell block. Cell Immunol 1978; 36:318-330.

    90. Darzynkiewicz Z, Andreeff M, Traganos F, Sharpless T, Melamed MR. Discrimination of cycling and noncycling lymphocytes by BrdUrd-suppressed acridine orange fluorescence in a flow cytometric system. Exp Cell Res 1978; 115:31-35.

    91. Andreeff M, Beck JD, Darzynkiewicz Z, Traganos F, Gupta S, Melamed MR, Good RA. RNA content in human lymphocyte subpopulations. Proc Natl Acad Sci USA 1978; 75:1938-1942.

    92. Ashihara T, Traganos F, Baserga T, Darzynkiewicz Z. A comparison of cell cycle related changes in post mitotic and quiescent AF8 cells as measured by flow cytometry after acridine orange staining. Cancer Res 1978; 38:2514-2518.

    93. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Differential Stainability of M vs G2 and G0 vs G1 cells. Chapter in: Pulse Cytophotometry III International Symposium, Lutz D, ed, European Press, Ghent, Belgium, pp. 262-273, 1978.

    94. Chelmicka-Schorr E, Arnason BGW, Astrom KE, Darzynkiewicz Z. Treatment of mouse muscular dystrophy with the protease inhibitor pepstatin. J Neuropathol Exp Neurol 1978; 37:263-268.

    1979

    95. Traganos F, Darzynkiewicz Z, Sharpless T, Melamed MR. Erythroid differentiation of Friend leukemia cells as studied by acridine orange staining and flow cytometry. J Histochem Cytochem 1979; 27:382-389.

    96. Darzynkiewicz Z, Traganos F, Andreeff M, Sharpless T, Melamed MR. Different sensitivity of chromatin to acid denaturation in quiescent and cycling cells as revealed by flow cytometry. J Histochem Cytochem 1979; 27:478-485.

    97. Collste L, Darzynkiewicz Z, Traganos F, Sharpless T, Whitmore WF, Melamed MR. Identification of polymorphonuclear leukocytes in cytological samples by flow cytometry. J Histochem Cytochem 1979; 27:390-394.

    98. Darzynkiewicz Z, Evenson D, Staiano-Coico L, Sharpless T, Melamed MR. Relationship between RNA content and progression of lymphocytes through the S phase of the cell cycle. Proc Natl Acad Sci USA 1979; 76:358-362.

    99. Collste LG, Darzynkiewicz Z, Traganos F, Devonec M, Claps MLK, Whitmore WF, Jr, Melamed MR. Cell cycle distribution of urothelial tumour cells as measured by flow cytometry. Br J Cancer 1979; 40:872-877.

    100. Collste LG, Darzynkiewicz Z, Traganos F, Sharpless T, Whitmore WF, Jr, Melamed MR. Regional lymph node reactivity in explanted bladder cancer of mice as measured by flow cytometry. Cancer Res 1979; 39:2120-2124.

    101. Evenson DP, Darzynkiewicz Z, Staiano-Coico L, Traganos F, Melamed MR. Effects of 9,10 Anthracendione, 1,4-bis 2-(hydroxyethyl)amino 2-ethyl amino-diacetate on cell survival and cell cycle progression in cultured mammalian cells. Cancer Res 1979; 39:2574-2581.

    102. Darzynkiewicz Z. Drug Effects on Cell Cycle Chapter in: Effects of Drugs on Cell Nucleus. Bush H et al. (Eds), Academic Press, New York, NY, pp. 470-474, 1979.

    103. Darzynkiewicz Z. Acridine Orange as a Molecular Probe in Studies of Nucleic Acids In Situ. Chapter in: Flow Cytometry and Sorting. Melamed MR, Mendelsohn M, Mullaney P (eds), John Wiley and Sons, New York, pp. 285-316, 1979.

    104. Darzynkiewicz Z, Evenson DP, Staiano-Coico L, Sharpless T, Melamed MR. Correlation between cell cycle duration and RNA content. J Cell Physiol 1979; 100:425-438.

    105. Drapkin RL, Andreeff M, Koziner B, Strife A, Wisniewski D, Darzynkiewicz Z, Melamed MR, Clarkson B. Subpopulation of human peripheral blood cells: Analysis of granulocytic progenitor cells by flow cytometry and immunologic surface markers. Am J Hematol 1979; 7:163-172.

    106. Eisinger M, Lee JS, Hefton JM, Darzynkiewicz Z, Chiao JW, deHarven E. Human epidermal cell cultures: Growth and differentiation in the absence of dermal components or medium supplements. Proc Natl Acad Sci USA 1979; 76:5340-5346..

    107. Collste LG, Kostyrka-Claps ML, Darzynkiewicz Z, Devonec M, Traganos F, Whitmore WF, Melamed MR. Lymph node reactivity to experimental bladder tumor in preimmunized animals as measured by two parameter flow cytometry. Invest Urol 1979; 17:191-194.

    108. Andreeff M, Beck JD, Darzynkiewicz Z, Arlin Z, Melamed MR, Clarkson B. DNA, RNA and chromatin structure assayed in situ by flow cytometry: A means for the classification of human leukemias. Verh Dtsch Krebs Ges 2. Gustave Fischer Verlag, Stuttgart, New York, p. 348, 1979.

    109. Andreeff M, Beck JD, Darzynkiewicz Z, Traganos F, Tan C, Clarkson B, Melamed MR. Blastic chronic myeloid leukemia: Cell proliferation in vivo, in tissue culture, and after desacetyl amide (DVA). Verh. Dtsch. Krebs. Ges. 2. Gustave Fischer Verlag, Stuttgart, New York, p. 351, 1979.

    1980

    110. Mora M, Darzynkiewicz Z, Baserga R. DNA synthesis and cell division in a mammalian cell mutant temperature sensitive for the processing of ribosomal RNA. Exp Cell Res 1980; 125:241-249.

    111. Andreeff M, Darzynkiewicz Z, Sharpless TK, Clarkson BD, Melamed MR. Discrimination of human leukemia subtypes by flow cytometric analysis of cellular DNA and RNA. Blood 1980; 55:282-293.

    112. Evenson DP, Traganos F, Darzynkiewicz Z, Staiano-Coico L, Melamed MR. Effects of 9,10-anthracenedione, 1,4-bis 2-(hydroxy-ethyl)amino ethyl amino-diacetate on cell morphology and nucleic acids of Friend leukemia cells. J Natl Cancer Inst 1980; 64:857-866.

    113. Traganos F, Evenson DP, Staiano-Coico L, Darzynkiewicz Z, Melamed MR. The action of dihydroxyanthraquinone on cell cycle progression and survival of a variety of cultured mammalian cells. Cancer Res 1980; 40:671-681.

    114. Collste LG, Darzynkiewicz Z, Traganos F, Sharpless T, Sogani P, Grabstald H, Whitmore WF, Jr. Melamed MR. Flow cytometry in cancer detection and evaluation using acridine orange metachromatic nucleic acid staining of irrigation cytology specimens. J Urol 1980; 123:478-485.

    115. Collste LG, Devonec M, Darzynkiewicz Z, Traganos F, Sharpless T, Whitmore WF, Jr, Melamed MR. Bladder cancer diagnosis by flow cytometry. Correlation between cell samples from biopsy and bladder irrigation fluid. Cancer 1980; 45:2389-2394.

    116. Evenson DP, Darzynkiewicz Z, Melamed MR. Comparison of human and mouse sperm chromatin by flow cytometry. Chromosoma 1980; 78:225-238.

    117. Pochron S, Rossini M, Darzynkiewicz Z, Traganos F, Baserga R. Failure of accumulation of cellular RNA in hamster cells stimulated to synthesize DNA by infection with Adenovirus 2. J Biol Chem 1980; 255:4411-4414.

    118. Traganos F, Staiano-Coico L, Darzynkiewicz Z, Melamed MR. Effects of ellipticine on cell cycle progression in cultured mammalian cells. Cancer Res 1980; 40:2390-2399.

    119. Petralia S, Soprano KJ, Pochron R, Darzynkiewicz Z, Traganos F, Baserga R. Increase in cellular RNA in cells infected with DNA oncogenic viruses. Cancer Res 1980; 40:3177-3180.

    120. Darzynkiewicz Z, Traganos F, Melamed MR. New cell cycle compartments identified by multiparameter flow cytometry. Cytometry 1980; 1:98-108.

    121. Evenson DP, Darzynkiewicz Z, Melamed MR. Relation of mammalian sperm chromatin heterogeneity to fertility. Science 1980; 210:1131-1133.

    122. Traganos F, Darzynkiewicz Z, Staiano-Coico L, Evenson D, Melamed MR. Rapid, automatic analysis of the cell cycle point of action of cytostatic drugs. Flow Cytometry IV, Proceedings Fourth Internatl. Symp. Flow Cytometry, Voss, Norway, June 4-8, Universitetforlaget, Bergen, 1980, pp. 511-515.

    123. Traganos F, Darzynkiewicz Z, Melamed MR. RNA content of the cell cycle. Flow Cytometry IV, Proceedings IVth Internatl. Symp. Flow Cytometry, Voss, Norway, June 4-8, Universitetforlaget, Bergen, 1980; pp. 331-335.

    124. Darzynkiewicz Z, Evenson D, Staiano-Coico L, Sharpless T, Melamed MR. Duration of the G1 and S phase of individual cells in populations is correlated with their RNA content. Flow Cytometry IV, Proceedings IVth Internatl. Symp. Flow Cytometry, Voss, Norway, June 4-8, Universitetforlaget, Bergen, 1980; pp. 336-340.

    125. Darzynkiewicz Z, Andreeff M, Traganos F, Melamed MR. Proliferation and differentiation of normal and leukemic lymphocytes as analyzed by flow cytometry. Flow Cytometry IV, Proceedings IVth Internatl. Symp. Flow Cytometry, Voss, Norway, June 4-8, Universitetforlaget, Bergen, 1980; pp. 392-397.

    126. Evenson DP, Darzynkiewicz Z, Melamed MR. Flow cytometric analysis of sperm chromatin structure. Flow Cytometry IV, Proceedings IVth Internatl. Symp. Flow Cytometry, Voss, Norway, June 4-8, Universitetforlaget, Bergen, 1980; pp.191-195.

    127. Darzynkiewicz Z, Sharpless T, Staiano-Coico L, Melamed MR. Subcompartments of the G1 phase of cell cycle detected by flow cytometry. Proc Natl Acad Sci USA 1980; 77:6696-6700.

    128. Traganos F, Evenson DP, Staiano-Coico L, Darzynkiewicz Z, Melamed MR. Ellipticine induced changes in cell growth and nuclear morphology. J Natl Cancer Inst 1980; 65:1329-1336.

    129. Traganos F, Staiano-Coico L, Darzynkiewicz Z, Melamed MR. Effects of prospidine on cell survival and growth of mammalian cells in culture. J Natl Cancer Inst 1980; 65:993-999, 1980.

    1981

    130. Melamed MR, Darzynkiewicz.Acridine Orange as a Quantitative Cytochemical Probe to Flow Cytometry. Chapter in: The Widening Horizons of Histochemistry. Polak, M.J. and Stoward, P.J. (eds.), John Wiley and Sons, New York, 1981; pp.237-261.

    131. Kapuscinski J, Darzynkiewicz Z, Traganos F, Melamed MR. Interactions of a new antitumor agent 1,4-dihydroxy-5-8-bis 2-(hydroxyethyl)amino)ethyl)amino)--9,10-anthracenedione with nucleic acids. Biochem Pharmacol 1981; 30:231-240.

    132. Darzynkiewicz Z, Traganos F, Xue S, Staiano-Coico L, Melamed MR. Rapid analysis of drug effects on the cell cycle. Cytometry 1981; 1:279-286.

    133. Traganos F, Staiano-Coico L, Darzynkiewicz Z, Melamed MR. Effects of dihydro-5-azacytidine on cell survival and cell cycle progression of cultured mammalian cells. Cancer Res 1981; 41:780-789.

    134. Darzynkiewicz Z, Traganos F, Melamed MR. Detergent treatment for an alternative to cell fixation for flow cytometry. J Histochem Cytochem 1981; 29:329-330.

    135. Darzynkiewicz Z, Staiano-Coico L, Melamed MR. Increased mitochondrial uptake of rhodamine 123 during lymphocyte stimulation. Proc Natl Acad Sci USA 1981; 78:2383-2387.

    136. Olszewski W, Darzynkiewicz Z, Rosen P, Schwartz MK, Melamed MR. Flow cytometry of breast carcinoma. I. Relation of DNA ploidy level to histology and estrogen receptors. Cancer 1981; 48:980-984.

    137. Olszewski W, Darzynkiewicz Z, Rosen P, Schwartz MK, Melamed MR. Flow cytometry of breast carcinoma. II. Relation of tumor cell cycle distribution to histology and estrogen receptor. Cancer 1981; 48:985-988..

    138. Traganos F, Staiano-Coico L, Darzynkiewicz Z, Melamed MR. Effects of aclacinomycin on cell survival and cell cycle progression of cultured mammalian cells. Cancer Res 1981; 41:2728-2737.

    139. Devonec M, Darzynkiewicz Z, Whitmore WF, Melamed MR. Flow cytometry for follow-up examinations of conservatively treated low stage bladder tumors. J Urol 1981; 126:166-170.

    140. Darzynkiewicz Z, Traganos F, Xue S, Melamed MR. Effect of n-butyrate on cell cycle progression and in situ chromatin structure of L1210 cells. Exp Cell Res 1981; 136:279-293.

    141. Traganos F, Darzynkiewicz Z, Melamed MR. Effects of the L-isomer (+)-1,2-bis (3,5-dioxopiperazine-1-yl) propane on cell survival and cell cycle progression of cultured mammalian cells. Cancer Res 1981; 41:4566-4576.

    142. Preisler H, and Darzynkiewicz Z. Flow cytometric analysis of proliferating and quiescent human leukemia cells. J Med 1981; 12:415-425.

    143. Darzynkiewicz Z, Andreeff M. Multiparameter flow cytometry. Part I. Application in analysis of the cell cycle. Clinical Bulletin MSKCC 1981; 11:47-87.

    144. Andreeff M. arzynkiewicz Z. Multiparameter flow cytometry. Part II. Application in clinical hematology. Clinical Bulletin MSKCC 1981; 11:120-130.

    1982

    145. Devonec M, Darzynkiewicz Z, Kostyrka-Claps ML, Collste L, Whitmore WF, Melamed MR. Flow cytometry of low stage bladder tumors: Correlation with cytologic and cystoscopic diagnosis. Cancer 1982; 49:109-118.

    146. Kapuscinski J, Darzynkiewicz Z, Melamed MR. Luminescence of the solid complexes of acridine orange with RNA. Cytometry 1982; 2:201-211.

    147. Traganos F, Darzynkiewicz Z, Melamed MR. The ratio of RNA to total nucleic acid content as a quantitative measure of unbalanced cell growth.Cytometry 1982; 2:212-218.

    148. Darzynkiewicz Z, Traganos F, Staiano-Coico L, Kapuscinski J, Melamed MR. Interactions of rhodamine 123 with living cells studied by flow cytometry. Cancer Res 1982; 42:799-806.

    149. Klein FA, Melamed MR, Whitmore WF, Herr HW, Sogani PC, Darzynkiewicz Z. Characterization of bladder papilloma by two-parameter DNA-RNA flow cytometry.Cancer Res 1982; 42:1094-1097.

    150. Darzynkiewicz Z, Traganos F. RNA Content and Chromatin Structure in Cycling and Non-cycling Cell Populations Studied by Flow Cytometry. Chapter in: The Genetic Expression in the Cell Cycle, Vol. 1. McCarthy KS, Sr. & Padilla GM (eds), Academic Press, New York, 1982; pp.103-128.

    151. Evenson DP, Darzynkiewicz Z, Melamed MR. Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility. J Histochem Cytochem 1982; 30:279-280.

    152. Olszewski W, Darzynkiewicz Z, Claps ML, Melamed MR. Flow cytometry of lung carcinoma: A comparison of DNA stemline and cell cycle distribution with histology. Anal Quant Cytol 1982; 4:90-94.

    153. Staiano-Coico L, Darzynkiewicz Z, Melamed MR, Weksler M. Changes in DNA content of human blood mononuclear cells with senescence. Cytometry 1982; 3:79-83.

    154. Darzynkiewicz Z, Crissman H, Traganos F, Stainkamp J. Cell heterogeneity during the cell cycle. J Cell Physiol 1982; 112:465-474.

    155. Olszewski W, Darzynkiewicz Z, Rosen PP, Claps ML, Melamed MR. Flow cytometry of breast carcinoma. III. Possible altered kinetics in axillary lymph node metastases. Anal Quant Cytol 1982; 4:275-278.

    1983

    156. Andreeff M, Darzynkiewicz Z: Chromatin structure in leukemia: Classification of subtypes and cell kinetics during induction. Cell Tissue Kinet 1983; 15: 679-681.

    157. Potmesil M, Levin M, Traganos F, Darzynkiewicz Z, Goldfeder A, Silber R. In vivo effects of adriamycin or N-trifluoroacetyladriamycin-14-valerate on mouse lymphoma. Eur J Cancer Clin Oncol 1983; 18:109-122.

    158. Piwnicka M, Darzynkiewicz Z, Melamed MR: RNA and DNA content of isolated nuclei measured by multiparameter flow cytometry. Cytometry 1983; 3:269-275.

    159. Darzynkiewicz Z, Traganos F, Melamed MR. Distinction between 5-bromodeoxyuridine labelled and unlabelled mitotic cells by flow cytometry. Cytometry 1983; 3:345-348.

    160. Darzynkiewicz Z. New cell cycle compartments distinguished by multiparameter flow cytometry. In: Proceedings of the 13th International Cancer Congress. Part C; Biology of Cancer (2); EA Mirand, WB Hutchinson & E Mihich (Eds), Alan R.Liss, New York, 1983, pp.171-177.

    161. Staiano-Coico L, Darzynkiewicz Z, Hefton JM, Dutkowski R, Darlington GJ, Weksler M. Increased sensitivity of lymphocytes from old humans to cell cycle arrest and chromosomal damage induced by 3H-TdR. Science 1983; 219:1335-1337.

    162. Darzynkiewicz Z, Evenson D, Kapuscinski J, Melamed MR. Denaturation of RNA and DNA in situ induced by acridine orange. Exp Cell Res 1983; 148:31-46.

    163. Kimmel M, Traganos F, Darzynkiewicz Z. Do all daughter cells enter the "indeterminate" ("A") state of the cell cycle? Analysis of stathmokinetic experiments. Cytometry 1983; 4:191-201.

    164. Kapuscinski J, Darzynkiewicz Z. Increased accessibility of bases in DNA upon binding of acridine orange. Nucleic Acids Res 1983; 11:7555-7568.

    165. Kapuscinski J, Darzynkiewicz Z, Melamed MR. Interactions of acridine orange with nucleic acids. Properties of complexes of acridine orange with single stranded RNA. Biochem Pharmacol 1983; 32:3679-3694.

    166. Gelfant S, Drewinko B, Darzynkiewicz Z, Eisinger M, Camplejohn RS. Cycling and noncycling human germinative epidermal cells. The continuous 3H-TdR labelling method in vivo and flow cytometric studies. Chapter in: Cell Proliferation and Psoriasis. Wright NA & Camplejohn RS. (Eds), Churchill Livingstone, London, 1983; pp.209-217.

    167. Higgins PJ, Darzynkiewicz Z, Melamed MR. Secretion of albumin and alpha fetoprotein by dimethylsulphoxide-stimulated hepatocellular carcinoma lines. Br J Cancer 1983; 48:485-493.

    168. Darzynkiewicz Z. Molecular interactions and cellular changes during the cell cycle. Pharmacol Ther 1983; 21:143-188.

    1984

    169. Higgins PJ, Piwnicka M, Darzynkiewicz Z, Melamed MR. Multiparameter flow cytometric analysis of the regenerative response of mouse hepatocytes to carbon tetrachloride-induced liver necrosis. Am J Pathol 1984; 115:31-35.

    170. Darzynkiewicz Z. Metabolic and kinetic compartments of the cell cycle distinguished by multiparameter flow cytometry. Chapter in: Growth, Cancer and the Cell Cycle. Skehan P & Friedman SI (eds), Humana Press, Clifton, New Jersey, 1984; pp.249-278.

    171. Darzynkiewicz Z, Williamson B, Carswell EA, Old LJ. The cell cycle specific effects of tumor necrosis factor. Cancer Res 1984; 44:83-90.

    172. Traganos F, Darzynkiewicz Z, Bueti C, Melamed MR. Effects of 1,4-bis(2'-chloroethyl)-1-4-diazabicyclo (2.2.1) heptane diperchlorate on cell survival and cell cycle progression of cultured mammalian cells. Cancer Invest 1984; 2:1-13.

    173. Darzynkiewicz Z, Carter S. Compound 48/80 impairs cytokinesis in murine leukemic cells. J Cell Physiol 1984; 119:1-6.

    174. Darzynkiewicz Z, Traganos F, Kapuscinski J, Staiano-Coico L, Melamed MR. Accessibility of DNA in situ to various fluorochromes: Relationship to chromatin changes during erythroid differentiation of Friend leukemia cells. Cytometry 1984; 5:355-363.

    175. Staiano-Coico L, Darzynkiewicz Z, Melamed MR, Weksler M. Immunological studies of aging. Impaired proliferation of T lymphocytes in elderly humans by flow cytometry. J Immunol 1984; 132:1788-1792.

    176. Kapuscinski J, Darzynkiewicz Z. Denaturation of nucleic acids induced by intercalating agents. Biochemical and biophysical properties of acridine orange-DNA complexes. J Biomol Struct Dyn 1984; 1:1485-1499.

    177. Traganos F, Bueti C, Darzynkiewicz Z, Melamed MR. Effects of retinoic acid versus dimethylsulfoxide on Friend erythroleukemia cell growth. I.Cell proliferation, RNA and protein content. J Natl Cancer Inst 1984; 72:193-204.

    178. Traganos F, Higgins PJ, Bueti C, Darzynkiewicz Z, Melamed MR. Effects of retinoic acid versus dimethylsulfoxide on Friend erythroleukemia cells. II. Induction of quiescent, nonproliferating cells. J Natl Cancer Inst 1984; 73:205-215.

    179. Staiano-Coico L, Wolf R, Darzynkiewicz Z, Whitmore WF, Melamed MR. RNA and DNA content of isolated nuclei from bladder irrigation specimens as measured by flow cytometry. Anal Quant Cytol 1984; 6:24-29.

    180. Czerniak B, Darzynkiewicz Z, Staiano-Coico L, Herz F, Koss LG. Expression of Ca antigen in relation to cell cycle of cultured human tumor cells. Cancer Res 1984; 44:4342-4346.

    181. Darzynkiewicz Z, Carter S, Kimmel M. Effects of tritium labelled uridine on the cell cycle progression of L1210 cells. Cell Tissue Kinet 1984; 17:641-655.

    182. Kimmel M, Darzynkiewicz Z, Arino O, Traganos F. Metabolic cell cycle model based on unequal division of RNA to daughter cells during cytokinesis. J Theor Biol 1984; 101:637-664, 1984.

    183. Kapuscinski J, Darzynkiewicz Z. Condensation of nucleic acids by intercalating aromatic cations. Proc Natl Acad Sci USA 1984; 81:7368-7372.

    184. Walle A, Suthanthivan M, Taufield PA, Yankiwer B, Rubin AL, Stenzel KA, Darzynkiewicz Z. Multiparameter analysis of cell activation in renal allograft recipients. Transplant Proc 1984; 16:1585-1587.

    1985

    185. Darzynkiewicz Z, Traganos F, Kapuscinski J, Melamed MR. Denaturation and condensation of DNA in situ induced by acridine orange. Relationship to chromatin structure of Friend erythroleukemia cells. Cytometry 1985; 6:195-207.

    186. Evenson DP, Lee JS, Darzynkiewicz Z, Melamed MR. Rhodamine 123 alters the mitochondrial ultrastructure of cultured L1210 cells. J Histochem Cytochem 1985; 33:353-359.

    187. Crissman HA, Darzynkiewicz Z, Tobey RA, Steinkamp JA. Correlated measurements of DNA, RNA and protein content in individual cells by flow cytometry. Science 1985; 228:1321-1324.

    188. Staiano-Coico L, Wolf R, Herr HW, Whitmore WFJr, Pinsky CM, Oettgen HF, Darzynkiewicz, Melamed MR. Monitoring intravesicular BCG treatment of urinary bladder carcinoma with flow cytometry. J Urol 1985; 133:786-788.

    189. Melamed J, Darzynkiewicz Z. RNA content and chromatin structure of CHO cells arrested in metaphase by Colcemid. Cytometry 1985; 6:381-385.

    190. Moran R, Darzynkiewicz Z, Staiano-Coico L, Melamed MR. Detection of BrdUrd incorporation by monoclonal antibodies: Role of the DNA denaturation step. J Histochem Cytochem 1985; 33:821-827.

    191. Crissman HA, Darzynkiewicz Z, Tobey RA, Steinkamp JA. Normal and perturbed growth of CHO cells. Correlated analysis of DNA, RNA and protein content by flow cytometry. J Cell Biol 1985; 101:141-147.

    192. Staiano-Coico L, Stollar D, Darzynkiewicz Z, Dutkowski R, Weksler ME. Binding of anti-Z DNA antibodies in quiescent and activated lymphocytes: Relation to cell cycle progression and chromatin changes. Mol Cell Biol 1985; 5:3270-3273.

    193. Kapuscinski J, Darzynkiewicz Z. Interactions of antitumor agents ametantrone and mitoxantrone (novantrone) with double-stranded DNA. Biochem Pharmacol 1985; 34:4203-4213.

    194. Eisinger M, Marko O, Darzynkiewicz Z, Houghton AK. Long-term cultures of normal human melanocytes in the presence of phorbol esters. Characterization of differentiated phenotype. In: Biological Molecular and Clinical Aspects of Pigmentation. Proc. XII Int. Pigment Cell Conference, Giessen, Germany. J Bagnara & SN Klaus (Eds). University of Tokyo Press, Tokyo, 1985; pp. 377-388.

    1986

    195. Evenson D, Darzynkiewicz Z, Jest L, Janca F, Ballachey B. Changes in accessibility of DNA in situ to various fluorochromes during spermatogenesis. Cytometry 1986; 7:45-53.

    196. Staiano-Coico L, Higgins PJ, Darzynkiewicz Z, Kimmel M, Gottlieb AB, Pagan-Charry Z, Madden MR, Finkelstein JL, Hefton JM. Human keratinocyte culture. Identification and staging of epidermal cell subpopulations. J Clin Invest 1986; 77:396-404.

    197. Darzynkiewicz Z, Traganos F, Kimmel M. Assay of Cell Cycle Kinetics by Multivariate Flow Cytometry. Chapter in: Techniques of Cell Cycle Analysis. Gray JW & Darzynkiewicz Z (eds), Humana Press, Inc., Clifton, New Jersey, 1986; pp. 291-332.

    198. Darzynkiewicz Z. Cytochemical Probes of Cycling and Quiescent Cells Applicable for Flow Cytometry. Chapter in: Techniques of Cell Cycle Analysis. Gray JW & Darzynkiewicz Z (eds), Humana Press, Inc., Clifton, New Jersey, 1986; pp. 225-279.

    199. Melamed MR, Enker WE, Janov AJ, Kessler G, Darzynkiewicz Z. Flow cytometry of colorectal carcinoma with three years follow-up. Col Rec 1986; 29:184-186.

    200. Darzynkiewicz Z, Traganos F, Staiano-Coico L. Cell and nuclear growth during G1: Kinetic and clinical implication. Ann NY Acad Sci 1986; 468:45-54.

    201. Kimmel M, Darzynkiewicz Z, Staiano-Coico L. Stathmokinetic analysis of human epidermal cells in vitro. Cell Tissue Kinet 1986; 19:289-304.

    202. Kapuscinski J, Darzynkiewicz Z. Relationship between the pharmacological activity of antitumor drugs Ametantrone and Mitoxantrone (Novatrone) and their ability to condense nucleic acids. Proc Natl Acad Sci USA 1986; 83:6302-6306.

    203. Darzynkiewicz Z, Kapuscinski J, Carter SP, Schmid F, Melamed MR. Cytostatic and cytotoxic properties of pyronin Y. Relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res 1986; 46:5760-5766.

    204. Darzynkiewicz Z, Staiano-Coico L, DeLeo AB, Old LJ. p53 content in relation to cell growth, proliferation and RNA content in murine L1210 leukemia and normal thymocytes. Leukemia Res 1986; 10:1383-1390, 1986.

    205. Darzynkiewicz Z. Cell Growth and Division Cycle. Chapter in: Intl Encycl Pharmacol Ther 1986; 121:1-43.

    1987

    206. Traganos F, Bueti C, Darzynkiewicz Z, Melamed MR. Effects of a new amsacrine derivative, N-5 dimethyl 9((2-methoxy-4-methylsulfonylamino)phenyl-amino))-4-acridine carboxamide on cultured mammalian cells. Cancer Res 1987; 47:424-432.

    207. Darzynkiewicz Z, Carter SP, Old LJ. Effect of recombinant tumor necrosis factor on HL-60 cells. Cell cycle specificity and synergism with actinomycin D. J Cell Physiol 1987; 130:328-335.

    208. Kapuscinski J, Darzynkiewicz Z. Interactions of pyronin Y (G) with nucleic acids. Cytometry 1987; 8:129-137.

    209. Darzynkiewicz Z, Kapuscinski J, Traganos F, Crissman HA. Application of pyronin Y (G) in cytochemistry of nucleic acids. Cytometry 1987; 8:138-145.

    210. Cayre Y, Wetzel-Raynal MC, Darzynkiewicz Z, Dorner MH. A model for intermediate steps in monocytic differentiation: c-myc, c-fms and ferritin as markers. Proc Natl Acad Sci USA 1987; 84:7619-7623.

    211. Darzynkiewicz Z, Traganos F, Carter SP, Higgins PJ. In situ factors affecting stability of the DNA helix in interphase nuclei and metaphase chromosome. Exp Cell Res 1987; 172:168-179.

    212. Traganos F, Bueti C, Melamed MR, Darzynkiewicz Z. Cytokinetic effects of the bi-functional antitumor intercalator-ditercalenium on Friend erythroleukemia cells. Leukemia 1987; 1:411-416.

    213. Kapuscinski J, Darzynkiewicz Z. Interactions of acridine orange with double stranded nucleic acids. Spectral and affinity studies. J Biomol Struct Dyn 1987; 5:127-142.

    214. Kunicka JE, Darzynkiewicz Z, Melamed MR. DNA in situ sensitivity to denaturation: A new parameter for flow cytometry of normal colonic epithelium and colon carcinoma. Cancer Res 1987; 47:3942-3947.

    215. Walle AJ, Suthanthiran M, Darzynkiewicz Z, Rubin AL, Stenzel KH. Cell cycle analysis of activated mononuclear blood cells of renal allograft recipients. Transplant Proc 1987; 19:2183-2184.

    216. Walle AJ, Suthanthiran M, Darzynkiewicz Z, Stenzel KH. Nucleic acids content of blood mononuclear cells of renal allograft recipients. In: Clinical Cytometry and Histometry. Burger G, Ploem JS, & Goerttler K (Eds), Academic Press, 1987, pp. 473-476.

    217. Traganos F, Kimmel M, Bueti C, Darzynkiewicz Z. Effects of inhibition of RNA or protein synthesis on CHO cell cycle progression. J Cell Physiol 1987; 133:277-283.

    218. Tobey RA, Crissman HA, Wilder ME, Traganos F, Darzynkiewicz Z. Sensitivity to X-irradiation in relation to cell size of CHO cells synchronized in early G1. Cell Tissue Kinet 1987; 20:363-366.

    1988

    219. Walle AJ, Wong GY, Rubin AL, Stenzel KH, Suthanthiran M, Darzynkiewicz Z. Altered nucleic acid content of mononuclear cells in blood of renal allograft recipients. Cytometry 1988; 9:177-182.

    220. Darzynkiewicz Z, Kapuscinski J. Condensation of DNA in situ in metaphase chromosomes induced by intercalating ligands and its relationship to chromosome binding. Cytometry 1988; 9:7-18.

    221. Darzynkiewicz Z, Carter SP. Photosensitizing effects of the tricyclic heteroaromatic cationic dyes pyronin Y and toluidine blue O (tolonium chloride). Cancer Res 1988; 48:1295-1299.

    222. Traganos F, Crissman HA, Darzynkiewicz Z. Staining with pyronin Y detects changes in conformation of RNA during mitosis and hyperthermia of CHO cells. Exp Cell Res 1988; 179:535-544.

    223. Kapuscinski J, Traganos F, Darzynkiewicz Z. Interactions of antitumor drug ditercalinium with nucleic acids in solutions and in situ. Cancer Lett 1988; 42:185-191.

    224. Darzynkiewicz Z. Flow cytometry in cytopathology - Overview and perspectives. Anal Quant Cytol Histol 1988; 10:459-461.

    225. Darzynkiewicz Z, Traganos F. Unstainable DNA in cell nuclei. Comparison of ten different fluorochromes. Anal Quant Cytol Histol 1988; 10:462-466.

    226. Darzynkiewicz Z, Carter SP, Mikulski SM, Shogen K. Cytostatic and cytotoxic effects of Pannon, a novel anticancer agent. Cell Tissue Kinet 1988; 21:169-182.

    227. Darzynkiewicz Z. Cellular RNA content, a feature correlated with cell kinetics and tumor prognosis. Leukemia 1988; 2:777-787.

    1989

    228. Darzynkiewicz Z, Carter SP. Thermal stability of nucleosomes studied in situ by flow cytometry. Effect of ionic strength and n-butyrate. Exp Cell Res 1989; 180:551-556.

    229. Traganos F, Bueti C, Kapuscinski J, Darzynkiewicz Z. The antitumor intercalating drug ditercalinium binds preferentially to RNA in Friend erythroleukemia cells. Leukemia 1989; 3:522-529.

    230. Myc A, Kunicka JA, Melamed MR, Darzynkiewicz Z. Effect of swainsonine on stimulation and cell cycle progression of human lymphocytes. Cancer Res 1989; 49:2879-2883.

    231. Bartkowiak J, Kapuscinski J, Melamed MR, Darzynkiewicz Z. Selective displacement of nuclear proteins by antitumor drugs having affinity to nucleic acids. Proc Natl Acad Sci USA 1989; 86: 5151-5154.

    232. Koyama M, Takahashi K, Chou T-C, Darzynkiewicz Z, Kapuscinski J, Kelly TR, Watanabe KA. Intercalating agents with covalent bond forming capability. Novel type of potential anticancer agents. 2. Derivatives of chrysophanol and emodin. J Med Chem 1989; 32: 1594-1599.

    233. Darzynkiewicz Z, Carter SP, Kapuscinski J, Watanabe KA. Effect of derivatives of chrysophanol, a new type of potential antitumor agent of anthraquinone family on growth and cell cycle of L1210 leukemic cells. Cancer Lett 1989; 46:181-187.

    234. Kunicka JE, Olszewski W, Rosen P, Kimmel M, Melamed MR, Darzynkiewicz Z. DNA in situ sensitivity to denaturation as a marker of human breast tumors. Cancer Res 1989; 49: 6347-6351.

    235. Bories D, Raynal M-C, Solomon DH, Darzynkiewicz Z, Cayre YE. Down-regulation of a serine protease, Myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells. Cell 1989; 59: 959-968.

    236. Staiano-Coico L, Darzynkiewicz Z, McMahon C. Cultured human keratinocytes: Discrimination of different cell cycle compartments based upon measurement of nuclear RNA or total cellular RNA content. Cell Tissue Kinet 1989; 22: 235-243.

    237. Czerniak B, Darzynkiewicz Z, Herz F, Wersto RP, Koss LG. Flow cytometry in clinical oncology: Cell cycle and DNA ploidy in assessing tumor behavior. Mater Med Pol 1989; 21:3-9.

    1990

    238. Kunicka JE, Myc A, Melamed MR, Darzynkiewicz Z. Caffeine affects the cell cycle progression and decreases stability of DNA helix in situ in L1210 cells. Cell Tissue Kinet 1990; 23:31-39.

    239. Kapuscinski J, Darzynkiewicz Z. Structure destabilization and condensation of nucleic acids by intercalators. Structure & Methods, Vol. 3: DNA & RNA. In: Proceedings of the 6th Conversation in the Discipline Biomolecular Stereodynamics. RH Sarma & MH Sarma (Eds), Adenine Press, Schenectady, NY, 1990, pp. 267-281.

    240. Darzynkiewicz Z. Probing Nuclear Chromatin by Flow Cytometry. Chapter in: Flow Cytometry and Sorting. Melamed MR, Mendelsohn M & Lindmo T (eds), Alan R Liss, Inc., New York. 1990, pp. 315-340.

    241. Darzynkiewicz Z, Traganos F. Multiparameter Flow Cytometry in Studies of the Cell Cycle. Chapter in: Flow Cytometry and Sorting. Melamed MR, Mendelsohn M & Lindmo T (eds), Alan R Liss, Inc., New York. 1990, pp. 469-502.

    242. Darzynkiewicz Z, Kapuscinski J. Acridine Orange, a Versatile Probe of Nucleic Acids and Other Cell Constituents. Chapter in: Flow Cytometry and Sorting. Melamed MR, Mendelsohn M & Lindmo T (eds), Alan R. Liss, Inc., New York. 1990, pp. 291-314.

    243. Evenson D, Darzynkiewicz Z. Acridine orange induced precipitation of mouse testicular sperm cell DNA reveals new patterns of chromatin structure. Exp Cell Res 1990; 187:328-334.

    244. Del BinoG, Skierski JS, Darzynkiewicz Z. Diverse effects of camptothecin, an inhibitor of topoisomerase I on the cell cycle of lymphocytic (L1210, MOLT-4) and myelogenous (HL-60, KG1) leukemic cells. Cancer Res 1990; 50:5746-5750.

    245. Darzynkiewicz Z. Acid-Induced Denaturation of DNA in situ as a Probe of Chromatin Structure. Chapter in: Methods in Cell Biology, Vol. 33. Flow Cytometry. Z Darzynkiewicz & HA Crissman (eds), Academic Press, New York, N.Y. 1990, pp. 337-352.

    246. Darzynkiewicz, Z. Differential Staining of DNA and RNA in Intact Cells and Isolated Cell Nuclei with Acridine Orange. Chapter in: Methods in Cell Biology. Vol.33. Flow Cytometry. Z Darzynkiewicz & HACrissman (eds), Academic Press, New York, N.Y. 1990, pp. 285-298.

    247. Kapuscinski J, Darzynkiewicz Z: Spectral Properties of Fluorochromes Commonly Used in Flow Cytometry. Chapter in: Methods in Cell Biology, Vol. 33. Flow Cytometry. Z Darzynkiewicz & HA Crissman (eds), Academic Press, New York, N.Y. 1990 pp., 655-669.

    248. Crissman HA, Darzynkiewicz Z, Steinkamp JA, Tobey RA. Simultaneous Fluorescent Labeling of DNA, RNA and Protein. Chapter in: Methods in Cell Biology. Vol.33. Flow Cytometry. Z Darzynkiewicz & HA Crissman (eds), Academic Press New York, N.Y. 1990, pp. 305-314.

    249. Myc A, DeAngelis P, Melamed MR, Darzynkiewicz Z. Retention of the mitochondrial probe rhodamine 123 in normal lymphocytes and leukemic cells in relation to the cell cycle. Exp Cell Res 1990; 192:198-202.

    1991

    250. Del Bino G, Lassota P, Darzynkiewicz Z. The S-phase cytotoxicity of camptothecin. Exp Cell Res 1991; 193:27-35.

    251. Lassota P, Melamed MR, Darzynkiewicz Z. Release of specific proteins from nuclei of HL-60 and MOLT-4 cells by antitumor drugs having affinity to nucleic acids. Biochem Pharmacol 1991; 41:1055-1065.

    252. Bretton PR, Darzynkiewicz Z, Henry E, Kimmel M, Fair WR, Melamed MR. DNA in situ sensitivity to denaturation in bladder cancer and its correlation with tumor stage.Cancer Res 1991; 50:7912-7914.

    253. Del Bino G, Darzynkiewicz Z. Camptothecin, teniposide or m-AMSA but not mitoxantrone or doxorubicin induce degradation of nuclear DNA in S-phase of HL-60 cells. Cancer Res 1991; 51:1165-1169.

    254. Myc A, DeAngelis P, Melamed MR, Darzynkiewicz Z. Effect of Swainsonine on Il-2 receptor expression and proliferation of human lymphocytes. Clin Exp Immunol 1991; 4:406-410.

    255. Traganos F, Kapuscinski J, Darzynkiewicz Z. Caffeine modulates the effects of DNA intercalating drugs in vitro: A flow cytometric and spectrophotometric analysis of caffeine interaction with Novantrone, doxorubicin, ellipticine and the doxorubicin analog, AD198. Cancer Res 1991; 51:3682-3689.

    256. Traganos F, Kaminska-Eddy B, Darzynkiewicz Z. Caffeine reverses the cytotoxic and cell kinetic effects of Novantrone (Mitoxantrone). Cell Prolif 1991; 24:305-319.

    257. Bruno S, Bauer K, Crissman HA, Darzynkiewicz Z. Changes in cell nuclei during S phase: Progressive chromatin condensation and altered expression of the proliferation-associated nuclear proteins Ki-67, p-105, cyclin/PCNA and p34. Exp Cell Res 1991; 196:99-106.

    258. Jakbisiak M, Bruno S, Skierski J, Darzynkiewicz Z. The cell cycle specific effects of lovastatin. Proc Natl Acad Sci USA 1991; 88:3628-3632.

    259. Darzynkiewicz Z. Multiparameter Analysis of the Cell Cycle by Flow Cytometry: Clinical Implications. Chapter in: Proc. Int. Symp. Flow Cytometry and Image Analysis for Clinical Applications. June 30-July 3, 1991, Elsevier, Amsterdam pp. 311-318.

    260. Darzynkiewicz Z, Traganos F, Kimmel M, Myc A. Kinetic and Metabolic Heterogeneity of Cells in the Cell Cycle. Chapter in: Stochastic Modelling in Biology. P Tautu (ed), World Scientific Publishing, Singapore 1991, pp. 30-50.

    261. Darzynkiewicz Z. Flow Cytometric Methods for RNA Content Analysis. Chapter in: Methods: A Companion to Methods in Enzymology. E Rothenberg (ed) Academic Press, New York. 1991; 2:200-206.

    262. Strife A, Lambek C, Perez A, Darzynkiewicz Z, Skierski J, Gulati S, Haley JD, Iwata KK, Clarkson BD. The effects of TGF-alpha on the growth of highly enriched hematopoietic progenitor cells derived from normal human bone marrow and peripheral blood. Cancer Res 1991; 51:4828-4836.

    263. Del Bino G, Skierski J, Darzynkiewicz Z. The concentration-dependent diversity of effects of DNA topoisomerase I and II inhibitors on the cell cycle of HL-60 cells. Exp Cell Res 1991; 195:485-491.

    1992

    264. Bruno S, Ardelt B, Skierski JS, Traganos F, Darzynkiewicz Z. Different effects of staurosporine, an inhibitor of protein kinases, on the cell cycle and chromatin structure of normal and leukemic lymphocytes. Cancer Res 1992; 52:470-473.

    265. Bruno S, Darzynkiewicz Z. Cell cycle dependent expression and stability of the nuclear protein detected by Ki-67 antibody in HL-60 cells.Cell Prolif 1992; 25:31-40.

    266. Rivas CI, Wisniewski D, Strife A, Perez A, Lambek C, Bruno S, Darzynkiewicz Z, Clarkson B. Constitutive expression of p53 protein in enriched normal human marrow blast cell populations. Blood, 1992; 79:1982-1986.

    267. Hotz MA, Del Bino G, Lassota P, Traganos F, Darzynkiewicz Z. Cytostatic and cytotoxic effects of the DNA topoisomerase II inhibitor, fostriecin, on human promyelocytic HL-60 and lymphocytic MOLT-4 leukemic cells. Cancer Res 1992; 52:1530-1535.

    268. Myc A, Traganos F, Lara J, Melamed MR, Darzynkiewicz Z. DNA stainability in aneuploid breast tumors: comparison of four DNA fluorochromes differing in binding properties. Cytometry 1992; 13:389-394.

    269. Darzynkiewicz Z. Mammalian Cell Cycle Analysis. Chapter in: The Cell Cycle: A Practical Approach. P Fantes & R Brooks (eds), Oxford University Press, Oxford, 1992, pp. 45-68.

    270. Bruno S, Giaretti W, Darzynkiewicz Z. Effect of camptothecin on mitogenic stimulation of human lymphocytes: Involvement of DNA topoisomerase I in cell transition from G0 to G1 phase of the cell cycle and in DNA replication. J Cell Physiol 1992; 151:478-486.

    271. Bruno S, Gorczyca W, Darzynkiewicz Z. The effect of ionic strength in immunocytochemical detection of the proliferation associated nuclear antigens p120, PCNA and the protein reacting with Ki-67 antibody. Cytometry 1992; 13:496-501.

    272. Hotz MA, Traganos F, Darzynkiewicz Z. Changes in nuclear chromatin related to apoptosis or necrosis induced by the DNA topoisomerase II inhibitor fostriecin in MOLT-4 and HL-60 cells are revealed by altered DNA sensitivity to denaturation. Exp Cell Res 1992; 201:184-191.

    273. Mikulski SM, Viera A, Darzynkiewicz Z. Synergism between a novel amphibian oocyte ribonuclease and lovastatin in inducing cytostatic and cytotoxic effects in human lung and pancreatic carcinoma cell lines. Br J Cancer 1992; 66:304-310.

    274. Bruno S, Lassota P, Giaretti W, Darzynkiewicz Z. Apoptosis of rat thymocytes triggered by prednisolone, camptothecin, or teniposide is selective to G0 cells and is prevented by inhibitors of proteases. Onc Res 1992; 4:29-35.

    275. Gorczyca W, Bruno S, Darzynkiewicz RJ, Gong J, Darzynkiewicz Z. DNA strand breaks occurring during apoptosis: Their early in situ detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors. Intl J Onc 1992; 1:639-648.

    276. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. Features of apoptotic cells measured by flow cytometry. Cytometry 1992; 13:795-808.

    277. Bruno S, Del Bino G, Lassota P, Giaretti W, Darzynkiewicz Z. Inhibitors of proteases prevent endonucleolysis accompanying apoptotic cell death. Leukemia 1992; 6:1113-1120.

    278. Del Bino G, Bruno S, Yi PN, Darzynkiewicz Z. Apoptotic cell death triggered by camptothecin or teniposide: The cell cycle specificity and effects of ionizing radiation. Cell Prolif 1992; 25:537-548.

    279. Black RR, Bouvier MM, SheuK-FR, Darzynkiewicz Z, Blass JP. Presence of typical neuronal markers in serially cultured cells from adult human brain. J Neurol Sci 1992; 111:104-112.

    280. Traganos F, Ardelt B, Halko NM, Bruno S, Darzynkiewicz Z. Effect of Genistein, a tyrosine kinase inhibitor, on the growth and cell cycle progression of normal human lymphocytes and human leukemic MOLT-4 and HL-60 cells. Cancer Res 1992; 52: 6200-6208.

    281. Capasso JM, Bruno S, Cheng W, Li P, Rodgers R, Darzynkiewicz Z, Anversa P. Ventricular loading is coupled with DNA synthesis in adult cardiac myocytes after acute and chronic myocardial infarction in rats. Circculation Res 1992; 71: 1379-1389.

    1993

    282. Traganos F, Knutti-Hotz J, Hotz M, Gorczyca W, Ardelt B, Darzynkiewicz Z. The protein kinase C inhibitor H7 blocks normal human lymphocyte stimulation and induces apoptosis of both normal lymphocytes and leukemic MOLT-4 cells. Intl J Onc 1993; 2:47-59.

    283. Strife A, Perez A, Lambek C, Wisniewski D, Bruno S, Darzynkiewicz Z, Clarkson B. Characterization of lineage-negative blast subpopulations derived from normal and chronic myelogenous leukemia bone marrows and determination of their responsiveness to human c-kit ligand. Cancer Res 1993; 53:401-409.

    284. Capasso JM, Bruno S, Zhang X, Darzynkiewicz Z, Anversa P. Myocyte DNA synthesis with aging: correlation with ventricular loading in rats. J Cell Physiol 1993; 155:635-648.

    285. Darzynkiewicz Z, Gorczyca W, Lassota P, Traganos F. Altered sensitivity of DNA in situ to denaturation in apoptotic cells. Ann NY Acad Sci 1993; 677:334-339.

    286. Darzynkiewicz Z. The Cell Cycle. Application of Flow Cytometry in Studies of Cell Reproduction. Chapter in: Flow Cytometry: Principles and Clinical Applications. KD Bauer, RE Duque & TV Shankey (eds), Williams & Wilkins, Baltimore, MD, 1993, pp. 13-40.

    287. Darzynkiewicz Z. Chromatin Structure Determination in situ Using AO. Chapter in: Handbook of Flow Cytometry Methods. JP Robinson, Z Darzynkiewicz, P Dean, L Dressler, H Tanke & L Wheeless (eds), Wiley-Liss, New York, NY. 1993, pp. 113-114.

    288. Darzynkiewicz Z. DNA-RNA Differential Staining Using AO. Chapter in: Handbook of Flow Cytometry Methods. JP Robinson, Z Darzynkiewicz, P Dean, L Dressler, H Tanke & L Wheeless (eds), Wiley-Liss, New York, NY. 1993, pp. 114-115.

    289. Darzynkiewicz Z. DNA Content Analysis Using DAPI DNA/Protein Staining Using a Combination of DAPI and Sulforhodamine 101. Chapter in: Handbook of Flow Cytometry Methods. JP Robinson, Z Darzynkiewicz, P Dean, L Dressler, H Tanke & L Wheeless (eds), Wiley-Liss, New York, NY. 1993, pp.116-117.

    290. Darzynkiewicz Z. Detection of DNA Strand Breaks Associated with Apoptosis. Chapter in: Handbook of Flow Cytometry Methods. JP Robinson, Z Darzynkiewicz, P Dean, L Dressler, H Tanke & L Wheeless (eds), Wiley-Liss, New York, NY. 1993, pp. 206-207.

    291. Gorczyca W, Melamed MR, Darzynkiewicz Z. Apoptosis of S phase HL-60 cells induced by DNA topoisomerase inhibitors: Detection of DNA strand breaks by flow cytometry using the in situ nick translation assay. Toxic Lett 1993; 67:249-258.

    292. Gorczyca W, Gong JP, Darzynkiewicz Z. Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res 1993; 53:1945-1951..

    293. Darzynkiewicz Z, Melamed MR. Flow cytometry and sorting: An historical perspective. Cytometry Res (Japan) 1993; 3:1-9.

    294. Gorczyca W, Gong J, Ardelt B, Traganos F, Darzynkiewicz Z. The cell cycle related differences in susceptibility of HL-60 cells to apoptosis induced by various antitumor agents. Cancer Res 1993; 53:3186-3192.

    295. Gorczyca W, Bigman K, Mittelman A, Ahmed T, Gong JP, Melamed MR, Darzynkiewicz Z. Induction of DNA strand breaks associated with apoptosis during treatment of leukemias. Leukemia 1993; 7: 659-670.

    296. Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells. Exp Cell Res 1993; 207:202-205.

    297. Gorczyca W, Ardelt B, Darzynkiewicz Z. Different rates of degradation of the proliferationo-associated nuclear and nucleolar antigens during apoptosis of HL-60 cells induced by DNA topoisomerase inhibitors. Int J Onc 1993; 3: 627-634.

    298. Gong JP, Traganos F, Darzynkiewicz Z. Simultaneous analysis of cell cycle kinetics at two different DNA ploidy levels based on DNA content and cyclin B measurements. Cancer Res 1993; 53:5096-5099.

    299. Traganos F, Kapuscinski J, Gong JP, Ardelt B, Darzynkiewicz RJ, Darzynkiewicz Z. Caffeine prevents apoptosis and cell cycle effects induced by camptothecin or topotecan in HL-60 cells. Cancer Res 1993; 53: 4613-4618.

    300. Strife A, Perez A, Lambek C, Wisniewski D, Bruno S, Darzynkiewicz Z, Clarkson B. Differences in the composition and in the efficiency of red cell production of normal and CML erythroid progenitor populations are highlighted by response to human c-kit ligand. Leukemia Res 1993; 17:799-807.

    301. Gong JP, Li X, Darzynkiewicz Z. Different patterns of apoptosis of HL-60 cells induced by cycloheximide and camptothecin. J Cell Physiol 1993; 157:263-270.

    302. Gorczyca W, Melamed MR, Darzynkiewicz Z. Programmed cell death (apoptosis). Patol Pol 1993; 44:113-119.

    303. Gorczyca W, Kram A, Tuziak T, Darzynkiewicz Z, Melamed MR.Proliferation Cell Nuclear Antigen in archival surgical specimens of malignant lymphoma and metastatic carcinoma: Immunohistochemical and flow cytometric analysis. Pat Pol 1993; 44:121-128.

    304. Gong JP, Traganos F, Darzynkiewicz Z. Expression of cyclins B and E in individual MOLT-4 cells and in stimulated human lymphocytes during their progression through the cell cycle. Int J Onc 1993; 3:1037-1042.

    305. Darzynkiewicz Z, Gorczyca W, Hotz MA, Lassota P, Bruno S, Traganos F. Chromatin Changes Accompanying Apoptosis Detected by Flow Cytometry. Chapter in: Flow Cytometry: New Developments. A. Jacquemin-Sablon (ed), Springer-Verlag, Paris; NATO ASI Series, 1993, pp. 311-326.

    306. Darzynkiewicz Z, Gorczyca W, Lassota P, Traganos F. Altered sensitivity of DNA in situ to denaturation in apoptotic cells. Chapter in: Clinical Flow Cytometry, AL Landay, KAAult, KD Bauer & PS Rabinovitch, eds. Ann NY Acad Sci Vol 677. NY. Ac Sci, New York, 1993, pp 334-340.

    307. Tillotson J, Darzynkiewicz Z, Cohen L, Ronai Z. Effects of linoleic-acid on mammary-tumor cell proliferation are associated with changes in p53 protein expression. Int J Oncol 1993; 3:81-87.

    1994

    308. Blass JP, Markersbury WR, Ko LW, De Giorgio L, Sheu KF, Darzynkiewicz Z. Presence of neuronal proteins in serially cultured cells from autopsy human brain. J Neurol Sci 1994; 121: 132-138.

    309. Del Bino G, Li X, Traganos F, Darzynkiewicz Z. Altered susceptibility of differentiating HL-60 cells to apoptosis induced by antitumor drugs. Leukemia 1994; 6: 281-288.

    310. Gorczyca W, Tuziak T, Kram A, Melamed MR, Darzynkiewicz Z. Detection of apoptosis in fine-needle aspiration biopsies by in situ end-labeling of fragmented DNA.Cytometry 1994; 15:169-175.

    311. Gong JP, Traganos F, Darzynkiewicz Z. Use of cyclin E restriction point to map cell arrest in G1 induced by n-butyrate, cycloheximide, staurosporine, lovastatin, mimosine and quercetin.Int J Oncol 1994; 4: 803-808.

    312. Garcia-Valenzuela E, Gorczyca W, Darzynkiewicz Z, Sharma SC. Apoptosis in adult retinal ganglion cells after axotomy. J Neurobiol 1994; 25:431-438.

    313. Hotz MA, Gong JP, Traganos F, Darzynkiewicz Z. Flow cytometric detection of apoptosis. Comparison of the assays of in situ DNA degradation and chromatin changes.Cytometry 1994; 15:237-244.

    314. Gong JP, Traganos F, Darzynkiewicz Z. A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal Biochem 1994; 218:314-319.

    315. Li X, Gong JP, Feldman E, Seiter K, Traganos F, Darzynkiewicz Z. Apoptotic cell death during treatment of leukemias. Leukemia and Lymphoma 1994; 13: Suppl 1, 65-70.

    316. Lassota P, Kazimierczuk Z, Darzynkiewicz Z. Apoptotic death of lymphocytes upon treatment with 2-chloro-2'-deoxyadenosine (2-CdA). Arch Immunol & Exp Ther 1994; 42:17-23.

    317. Traganos F, Gong JP, Ardelt B, Darzynkiewicz Z. Effect of staurosporine on MOLT-4 cell progression through G2 and on cytokinesis. J Cell Phys 1994; 158:535-544.

    318. Gong JP, Traganos F, Darzynkiewicz Z. Staurosporine blocks cell progression through G1 between cyclin D and cyclin E restriction points. Cancer Res 1994; 54:3136-3139.

    319. Li X, Traganos F, Melamed MR, Darzynkiewicz Z. Detection of 5-bromo-2-deoxyuridine incorporated into DNA by labeling strand breaks induced by photolysis (SBIP). Int J Oncol 1994; 4:1157-1161.

    320. Gong JP, Ardelt B, Traganos F, Darzynkiewicz Z. Unscheduled expression of cyclin B1 and cyclin E in several leukemic and solid tumor cell lines. Cancer Res 1994; 54:4285-4288..

    321. Li X, Traganos F, Darzynkiewicz Z. Simultaneous analysis of DNA replication and apoptosis during treatment of HL-60 cells with camptothecin and hyperthermia and mitogen stimulation of human lymphocytes. Cancer Res 1994; 54:4289-4293.

    322. Li X, Patel B, Melamed MR, Darzynkiewicz Z. The cell cycle effects and induction of apoptosis by 5-bromouridine in cultures of human leukemic MOLT-4 and HL-60 cell lines and mitogen stimulated normal lymphocytes, Cell Prolif 1994; 27: 307-319.

    323. Kajstura J, Zhang X, Reiss K, Szoke E, Li P, Lagrasta C, Cheng W, Darzynkiewicz Z, Olivetti G, Anversa, P. Myocyte cellular hyperplasia and myocyte cellular hypertrophy contribute to chronic ventricular remodeling in coronary artery narrowing induced cardiomyopathy in rats. Circulation Res 1994; 74:383-400.

    324. Black RS, DeGiorgio LA, Sheu K-FR, Darzynkiewicz Z, Duffy JT, Blass JP. Expression of neuronal proteins in cells from normal adult brain propagated in serial culture. J Neurochemistry 1994; 62:2132-2140.

    325. Gong JP, Li X, Traganos F, Darzynkiewicz Z. Expression of G1 and G2 cyclins measured in individual cells by multiparameter flow cytometry; a new tool in the analysis of the cell cycle. Cell Prolif 1994; 27: 357-371.

    326. Wang Z, Dudhane A, Orlikowsky T, Clarke K, Li X, Darzynkiewicz Z, Hoffman MK.CD4 engagement in vivo induces apoptosis in T cells. Eur J Immunol 1994; 24:1549-1552.

    327. Darzynkiewicz Z. Cell Cycle Analysis by Flow Cytometry. Chapter in: Handbook of Cell Biology, J Celis (ed), Academic Press, 1994, pp 261-271.

    328. Darzynkiewicz Z, Li X, Gong JP.Assays of cell viability. Discrimination of cells dying by apoptosis. Methods Cell Biol. 1994, 41:15-38.

    329. Darzynkiewicz Z. Acid-induced denaturation of DNA in situ as a probe of chromatin structure. Methods Cell Biol. 1994, 41: 527-542.

    330. Darzynkiewicz Z. Simultaneous analysis of cellular RNA and DNA content. Methods Cell Biol. 1994, 41: 401-420.

    331. Traganos F, Darzynkiewicz Z. Lysosomal proton pump activity: supravital cell staining with acridine orange differentiates leukocyte subpopulations.Methods Cell Biol. 1994, 41:185-194.

    332. Darzynkiewicz Z, Traganos F, Gong JP. Analysis of DNA content and cyclin protein expression in studies of DNA ploidy, growth fraction, lymphocyte stimulation and the cell cycle. Methods Cell Biol. 1994, 41: 421-436.

    1995

    333. Halicka DH, Ardelt B, Li X, Melamed MR, Darzynkiewicz Z. 2-deoxyglucose enhances sensitivity of human histiocytic U 935 cells to apoptosis induced by Tumor Necrosis Factor. Cancer Res., 1995; 55:444-449.

    334. Gong JP, Traganos F, Darzynkiewicz Z. Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry. Leukemia 1995; 9:893-899.

    335. Li X, Traganos F, Melamed MR, Darzynkiewicz Z. Single step procedure for DNA strand break labeling. Detection of apoptosis and DNA replication. Cytometry 1995; 20:172-180.

    336. Bhatia U, Danishefsky K, Traganos F, Darzynkiewicz Z. Induction of apoptosis and cell cycle specific change in expression of p53 in normal lymphocytes and MOLT-4 leukemic cells by nitrogen mustard. Clinical Cancer Res 1995; 1: 873-880.

    337. Li X, James WM, Traganos F, Darzynkiewicz Z. Application of biotin, digoxygenin or fluorescein conjugated deoxynucleotides to label DNA strand breaks in analysis of cell proliferation and apoptosis by flow cytometry. Biotech Histochem 1995; 70:235-242.

    338. Qiao L, Gorczyca W, Pizzolo JG, Melamed MR, Darzynkiewicz Z. Flow cytometric characterization of proliferation-associated nuclear antigen (p105) during the cell cycle in normal lymphocytes and promyelocytic leukemia cells. Anal Quant Cytol Histol 1995; 17:183-188.

    339. Darzynkiewicz Z. Apoptosis in antitumor strategies: Modulation of cell cycle or differentiation. J Cell Biochem 1995; 58:151-159.

    340. Murakami T, Li X, Gong JP, Bhatia U, Traganos F, Darzynkiewicz Z. Induction of apoptosis by 5-azacytidine. Drug concentration dependent differences in cell cycle specificity. Cancer Res 1995; 55:3093-3098.

    341. Gong JP, Traganos F, Darzynkiewicz Z. Threshold expression of cyclin E but not D type cyclins characterizes normal and tumor cells entering S phase. Cell Prolif 1995; 28:337-346.

    342. Bhatia U, Traganos F, Darzynkiewicz Z. Induction of cell differentiation potentiates apoptosis triggered by prior exposure to DNA damaging drugs. Cell Growth & Different 1995; 6:937-944.

    343. Gong JP, Traganos F, Darzynkiewicz Z. Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins B1 and A. Exp Cell Res 1995; 220:226-231.

    344. Venturelli D, Martinez R, Melotti P, Casella I, Peschle C, Cucco C, Spaminato G, Darzynkiewicz Z, Calabretta B. Overexpression of DR-nm23, a protein encoded by a member of nm23 family, inhibits granulocyte differentiation and induces apoptosis in 32Dc13 myeloid cells. Proc Natl Acad Sci USA 1995; 35-7439.

    345. Gong JP, Traganos F, Darzynkiewicz Z. Growth imbalance and altered expression of cyclins B1, A. E and D3 in MOLT-4 cells synchronized in the cell cycle by inhibitors of DNA replication. Cell Growth & Different 1995; 6: 1485-1493.

    346. Ronai Z, Tillotson JK, Traganos F, Darzynkiewicz Z, Conaway C, UpadhyayaP, El-Bayoumy K. Effects of organic and inorganic selenium compounds on rat mammary tumor cells. Int J Cancer 1995; 63:428-434.

    347. Li X, Darzynkiewicz Z. Labeling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation. Cell Prolif 1995; 28:571-579.

    348. Hara S, Tomita M, Darzynkiewicz Z. A flow cytometric study of correlation between heat induced apoptosis and heat shock protein 70 in HL-60 cells.Cytometry Res 1995; 5:83-88.

    349. Seiter K, Feldman EJ, Traganos F, Li X, Halicka HD, Darzynkiewicz Z, Lederman CA, Romero M-B, Ahmed T. Phase I study on Taxol in acute leukemis: Evaluation of in vivo induction of apoptosis by Taxol. Leukemia 1995; 9: 1961-1963.

    350. Skorski T, Nieborowska-Skorska M, Campbell K, Iozzo RV, Zon G, Darzynkiewicz Z, Calabretta B. Leukemia treatment in severe combined immunodeficiency mice by antisense oligonucleotides targeting cooperating oncogenes. J Exp Med 1995; 182: 1645-1653.

    351. Darzynkiewicz Z, Hara S, Murakami T, Numa Y, Li X. Flow cytometric analysis of cell death: A critical review. Cytometry Research 1995; 5:1-8.

    352. Darzynkiewicz Z, Li X, Gong JP, Hara S, Traganos F. Analysis of Cell Death by Flow Cytometry. Chapter in: Cell Growth and Apoptosis. A practical Approach. 2nd edition. G Studzinski (ed), Oxford University Press, Oxford, UK, 1995; pp. 143-168.

    353. Darzynkiewicz Z. Analysis of cell death by flow cytometry. Chapter in: Flow Cytometry:New Developments. A Jacquemin-Sablon (ed), Springer-Verlag, Paris; NATO ASI Series, Vol. 95. 1995; pp. 115-130.

    1996

    354. Li X, Melamed MR, Darzynkiewicz Z. Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color. Exp Cell Res 1996; 222:28-37.

    355. Hara S, Halicka HD, Bruno S, Gong J, Traganos F, Darzynkiewicz Z. Effect of protease inhibitors on early events of apoptosis. Exp Cell Res 1996; 223:372-384.

    356. Juan G, Gong JP, Traganos F, Darzynkiewicz Z. Unscheduled expression of cyclins D1 and D3 in human tumor cell lines. Cell Prolif 1996; 29:259-266.

    357. Juan G, Pan W, Darzynkiewicz Z. DNA segments sensitive to single strand specific nucleases are present in chromatin of mitotic cells. Exp Cell Res 1996; 227:197-202.

    358. Bruno S, Traganos F, Darzynkiewicz Z. Cell cycle synchronizing properties of staurosporine. Meth Cell Sci 1996; 18:99-107.

    359. Darzynkiewicz Z, Gong JP, Juan G, Ardelt B, Traganos F. Cytometry of cyclin proteins. Cytometry 1996; 25:1-13.

    360. Darzynkiewicz Z, Bruno S, Del Bino G, Traganos F. The cell cycle effects of camptothecin. Ann NY Acad Sci 1996; 803:93-100.

    361. Traganos F, Seiter K, Halicka HD, Darzynkiewicz Z. Induction of apoptosis by camptothecin and topotecan. Ann NY Acad Sci 1996; 803:101-110.

    362. Gorczyca W, Melamed MR, Darzynkiewicz Z. Laser scanning cytometer analysis of fraction of labeled mitoses. Cell Prolif 1996; 29:539-548.

    363. Numa Y, Matsudaira T, Kawamoto K, Darzynkiewicz Z. Induction of apoptosis in HL-60 (Human Leukemia-60) cells by MX-2 (new morpholino anthracyclin). Cytometry Res (Japan) 1996; 6:65-70.

    364. Darzynkiewicz Z. Flow cytometric detection of apoptosis and necrosis. In: Proceedings of the Xth International Congress of Histochemistry and Cytochemistry. Kyoto, Japan. Acta Histochemica Cytochemica 1996; Vol 29, Suppl. pp. 46-47.

    365. Murakami T, Sasaki K, Traganos F, Darzynkiewicz Z. Flow cytometric and laser scanning cytometric analysis of apoptosis induced by 5'azacytidine. In: Proceedings of the Xth International Congress of Histochemistry and Cytochemistry. Kyoto, Japan. Acta Histochemica Cytochemica 1996; Vol 29. Suppl. pp. 48-49.

    366. Darzynkiewicz Z. Detection of Apoptosis by Flow Cytometry. In: "Educational Program of the 26th Congress of the International Society of Hematology". Singapore, 25-29 Aug, 1996.

    367. Darzynkiewicz Z, Li X. Meaurements of cell death by flow cytometry. Chapter in: Techniques in Apoptosis: A users' guide. TG Cotter & SJ Martin eds. Portland Press, London, UK, 1996; pp. 71-106.

    368. Melamed MR. Darzynkiewicz Z. Flow cytometry in cytopathology. Chapter in: Comprehensive Cytopathology. Second Edition. M Bibbo (ed) WB Saunders, Philadelphia, PA, 1996; pp. 1023-1032.

    1997

    369. Seiter K, Feldman E J, Halicka D, Traganos F, Darzynkiewicz Z, Lake D, Ahmed T. Phase I clinical and laboratory evaluation of topotecan and cytoarabine in patients with acute leukemia. J Clin Oncol 1997; 15:44-55.

    370. Vibhakar R, Juan G, Traganos F, Darzynkiewicz Z, Finger L. Activation induced expression of human programmed death-1 gene in T-lymphocytes. Exp Cell Res 1997; 232: 25-28.

    371. Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F. Cytometry in cell necrobiology. Analysis of apoptosis and accidental cell death (necrosis).Cytometry 1997; 27:1-20.

    372. Gorczyca W, Darzynkiewicz Z, Melamed MR. Laser scanning cytometry in pathology of solid tumors. Acta Cytologica 1997; 41:98-108.

    373. Halicka HD, Seiter K, Feldman EJ, Traganos F, Mittelman A, Ahmed T, Darzynkiewicz Z. Cell cycle specificity of apoptosis during treatment of leukemias. Apoptosis 1997; 2:25-39.

    374. Juan G, Li X, Darzynkiewicz Z. Correlation between DNA replication and expression of cyclins A and B1 in individual MOLT-4 cells. Cancer Res 1997; 57:803-807.

    375. Haider SR, Juan G, Traganos F, Darzynkiewicz Z. Immuno-separation and immuno-detection of nucleic acids with halogenated nucleotides. Exp Cell Res 1997; 234:498-506.

    376. Halicka HD, Ardelt B, Juan G, Mittelman A, Chen S, Traganos F, Darzynkiewicz Z. Apoptosis and cell cycle effects induced by extracts of the Chinese herbal preparation PC SPES. Int J Oncol 1997; 11:437-448.

    377. Gorczyca W, Sorano V, Juan G, Melamed MR, Darzynkiewicz Z. Laser scanning analysis of cyclin B1 in primary human malignancies. Modern Pathol 1997; 10: 457-462

    378. Darzynkiewicz Z. Induction of apoptosis during treatment of leukemias. Relationship to the cell cycle. Acta Haematol Pol 1997; 28, Suppl. 2:9-21.

    379. Bedner E, Burfeind P, Gorczyca W, Melamed MR, Darzynkiewicz Z. Laser scanning cytometry distinguishes lymphocytes, monocytes and granulocytes by differences in their chromatin structure. Cytometry 1997; 29:191-196..

    380. Sailer BL, Valdez JG, Steinkamp JA, Darzynkiewicz Z, Crissman HA. Monitoring uptake of ellipticin and its fluorescence lifetime in relation to the cell cycle phase by flow cytometry. Exp Cell Res 1997; 236:259-267.

    381. Darzynkiewicz Z, Li X, Gong JP, Traganos F. Methods for analysis of apoptosis by flow cytometry.Chapter in: Manual of Clinical Laboratory Immunology. 5th Edition, ASM Press, Washington, DC. 1997; pp. 334-343

    382. Darzynkiewicz Z. Nucleic Acid Analysis. Introduction. Section in: Current Protocols in Cytometry. P Robinson, Z Darzynkiewicz, P Dean, L Dressler, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, NY. 1997; pp. 7.0.1-7.0.2.

    383. Darzynkiewicz Z, Juan G. Introduction and Overview of Nucleic Acid Analysis. Section in: Current Protocols in Cytometry. P Robinson Z. Darzynkiewicz, P Dean, L Dressler, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, NY. 1997; pp. 7.1.1-7.1.4.

    384. Darzynkiewicz Z, Juan G. Differential Staining of DNA and RNA. Section in: Current Protocols in Cytometry. P Robinson, Z Darzynkiewicz, P Dean, L Dressler, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, N.Y.1997; pp. 7.3.1-7.3.13.

    385. Darzynkiewicz Z, Juan G. Analysis of DNA Content and DNA Strand Breaks for Detection of Apoptotic Cells. Section in: Current Protocols in Cytometry. P Robinson, Z Darzynkiewicz, P Dean, A Orfao, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, NY. 1997; pp. 7.4.1-7.4.8.

    386. Darzynkiewicz Z, Juan G. DNA Content Measurement for DNA Ploidy and Cell Cycle Analysis. Section on: Current Protocols in Cytometry. P Robinson, Z Darzynkiewicz, P Dean, A Orfao, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, NY. 1997; pp. 7.5.1-7.5.24

    387. Darzynkiewicz Z. Methods in Analysis of Apoptosis and Necrosis. Chapter in: Advaced Methodologies in Flow Cytometry. A Cossarizza, Ed. Universita Degli Studi di Modena, Modena, Italy. 1997. pp 9-34.

    1998

    388. Deptala A, Halicka HD, Ardelt B, Ardelt W, Mikulski SM, Shogen K, Darzynkiewicz Z. Potentiation of tumor necrosis factor induced apoptosis by onconase. Int J Oncol 213: 11-16, 1998

    389. Juan G, Traganos F, James WM, Ray JM, Roberge M, Sauve DM, Anderson H, Darzynkiewicz Z. Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis. Cytometry 1998; 32:71-77.

    390. Juan G, Gruenwald S, Darzynkiewicz Z. Phosphorylation of retinoblastoma susceptibility gene protein assayed in individual lymphocytes during their mitogenic stimulation. Exp Cell Res 1998; 239:104-110.

    391. Hara S, Tagawa Y, Ayabe H, Darzynkiewicz Z. Analysis of of drug-induced apoptosis in human leukemic cell line HL-60. Gan To Kagaku Ryoho (Jap) 1998; Suppl. 3:393-399.

    392. Bedner E, Burfeind HsiehT-C, Wu JM, Aguero-Rosenfeld MA, Melamed MR, Horowitz HW, Wormser GP, Darzynkiewicz Z. Cell cycle effects and induction of apoptosis caused by infection of HL-60 cells with human granulocytic ehrlichiosis (HGE) pathogen measured by flow and laser scanning cytometry (LSC). Cytometry 1998; 33:47-55.

    393. Bedner E, Melamed MR, Darzynkiewicz Z. Time resolved kinetic reactions measured in individual cells by laser scanning cytometry (LSC) Cytometry 1998; 33:1-9.

    394. Juan G, Ardelt B, Mikulski SM, Shogen KM, Ardelt W, Mittelman A, Darzynkiewicz Z. G1 arrest of U-937 cells by onconase is associated with suppression of cyclin D3 expression, induction of p16INK4A, p21WAF1/CIP1 and p27KIP and decreased pRb phosphorylation. Leukemia 1998; 12:1241-1248.

    395. Darzynkiewicz Z, Bedner E, Traganos F, Murakami T. Critical aspects in analysis of apoptosis and necrosis. Human Cell 1998; 11:3-12.

    396. Juan G, Darzynkiewicz Z. Phosphorylation of pRb in individual HL-60 cells during their proliferation and differentiation. Exp Cell Res 1998; 244:83-92.

    397. Deptala A, Bedner E, Gorczyca W, Darzynkiewicz Z. Simple assay of activation of nuclear factor kappa B (NF-?B) by laser scanning cytometry (LSC). Cytometry 1998; 33:376-382.

    398. Mikulski S, Viera A, Deptala A, Shogen K, Darzynkiewicz Z. Anti-tumor activity synergism between onconase and proteasome inhibitors. Int J Oncol 1998; 13:633-644.

    399. Feldman E, Seiter K, Chiao JW, Halicka HD, Traganos F, Fatora SR, McMichael J, Baskind P, Goff H, Beer M, Ahmed T, Darzynkiewicz Z. In vitro and clinical evaluation of a human chorionic gonadotrophin praparation in acute leukemia. Leukemia 1998; 12:1749-1755.

    400. Juan G, Darzynkiewicz Z. Phosphorylation of retinoblastoma protein (pRb) assayed in individual cells by multilaser flow cytometry. Clinical Immunol Newsletter 1998; 18:89-94.

    401. Gorczyca W, Bedner E, Burfeind P, Darzynkiewicz Z, Melamed MR. Analysis is apoptosis in solid tumors by laser scanning cytometry. Mod Pathol 1998; 11:1-7.

    402. Gorczyca W, Melamed MR, Darzynkiewicz Z. Analysis of apoptosis by flow cytometry. Methods Mol Biol 1998; 91:217-238

    403. Gorczyca W, Davidian M, Gherson J, Ashikari R, Darzynkiewicz Z, Melamed MR. Laser scanning cytometry quantification of estrogen receptors in breast cancer. Anal Quant Cytol Histol 1998; 20:470-476.

    404. Darzynkiewicz Z, Juan G. Analysis of DNA denaturation. Section in: Current Protocols in Cytometry. P Robinson, Z Darzynkiewicz, P Dean, A Orfao, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, NY. 1998. pp. 7.8.1-7.8.4.

    405. Darzynkiewicz Z, Juan G. Analysis of DNA content and BrdUrd incorporation. Dection in: Current Protocols in Cytometry. P Robinson, Z Darzynkiewicz, P Dean, A Orfao, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, NY.1998. pp. 7.7.1 - 7.7.6.

    406. Juan G, Darzynkiewicz Z. Bivariate analysis of DNA content and expression of cyclin proteins. Section in: Current Protocols in Cytometry. P Robinson, Z Darzynkiewicz, P Dean, A Orfao, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, NY.1998. pp. 7.9.1 - 7.9.4.

    407. Juan G, Darzynkiewicz Z. Cell Cycle Analysis by Flow Cytometry. Chapter in: Handbook of Cell Biology, 2nd Edition, J Celis (ed.) Academic Press, San Diego CA 1998. pp. 261-274.

    408. Juan G, Darzynkiewicz Z. In situ DNA strand break labeling for the analysis of apoptosis and cell proliferation. Chapter in: Handbook of Cell Biology, 2nd Edition, J Celis (ed.) Academic Press, San Diego, CA, 1998. pp. 341-350.

    409. Mazur L, Darzynkiewicz Z. Apoptosis in hematopoetic system. Chapter in: Blood Physiology: Selected Topics. Z. Dabrowski, Ed., "Wydawnictwo Naukowe PWN", Warsaw, Poland, (in Polish), 1998; pp. 286-315 .

    410. Juan G, Darzynkiewicz Z. Detection of cyclins in individual cells by flow and laser scanning cytometry. Chapter in: Methods in Molecular Biology Vol. 91. Flow Cytometry Protocols. MJ Jaroszeski & R Heller, Eds. Humana Press, Totowa, NJ. 1998, pp. 67-75.

    411. Gorczyca W, Darzynkiewicz Z. Analysis of apoptosis by flow cytometry. Chapter in: Methods in Molecular Biology. Vol. 91. Flow Cytometry Protocols. MJ, Jaroszeski & R Heller, Eds. Humana Press, Totowa, NJ. 1998, pp. 217-238.

    412. Darzynkiewicz Z, Traganos F. Measurement of apoptosis. Chapter in: Advances in Biochemical Engineering/Biotechnology. Apoptosis. M Al-Rubeai, Ed. 1998, pp. 33-73.

    413. Darzynkiewicz Z, Bedner E, Burfeind P, Traganos F. Analysis of apoptosis by flow and laser scanning cytometry. Chapter in: Apoptosis. Detection and Assay Methods. Biotechniques Series on Molecular Laboratory Methods, No. 2. L Zhu & G Zhang, Eds. BioTechiques Books (Division of Eaton Publishing) Palo Alto, CA, 1998, pp. 75-92.

    1999

    414. Del Bino G, Darzynkiewicz Z, Degraef C, Mosselmans R, Galand P. Comparison of methods based on annexin V binding, DNA content or TUNEL for evaluating cell death in HL-60 and adherent MCF-7 cells.Cell Prolif 1999; 32:25-37.

    415. Bedner E, Li X, Gorczyca W, Melamed MR, Darzynkiewicz Z. Analysis of apoptosis by laser scanning cytometry. Cytometry 1999; 35:181-195.

    416. Tan L, Kowalska MA, Romo G, Lopez G, Darzynkiewicz Z, Niewiarowski S. Identification and characterization of endothelial glycoprotein Ib using Viper venom proteins. Blood 1999; 93:2605-2616.

    417. Juan G, Chang W, Traganos F, Darzynkiewicz Z. Histone H3 phosphorylation in human monocytes and during HL-60 cell differentiation. Exp Cell Res 1999; 246:212-229.

    418. Darzynkiewicz Z. The butler did it: Search for killer(s) of Kaposi's sarcoma cells in preparations of human chorionic gonadotropin. JNCI 1999; 91:104-106.

    419. Bedner E, Halicka HD, Cheng W, Salomon T, Deptala A, Gorczyca W, Melamed MR, Darzynkiewicz Z. High affinity binding of fluorescein isothiocyanate to eosinophils detected by laser scanning cytometry. A potential source of error in analysis of blood samples utilizing fluorescein-conjugated reagents in flow cytometry. Cytometry 1999; 36:77-82.

    420. Darzynkiewicz Z. Bedner E. Gorczyca W, Melamed MR. Laser scanning cytometry. A new instrumentation with many applications. Exp Cell Res 1999; 249:1-12.

    421. Hsieh T-C, Juan G, Darzynkiewicz Z, Wu JM. Resveratrol increases nitric oxide synthase, induces accumulation of p53 and p21WAF1/CIP1 and suppresses cultured bovine pulmonary artery endothelial cell proliferation by perturbing progression through S and G2. Cancer Res 1999; 59:2596-2601.

    422. Li X, Darzynkiewicz Z. The Schrdinger's cat quandary in cell biology: Integration of live cell functional assays with measurements of fixed cells in analysis of apoptosis. Exp Cell Res 1999; 249: 404-412.

    423. Hsieh T-C, Burfeind P, Laud K, Backer JM, Traganos F, Darzynkiewicz Z, Wu JM. Cell cycle effects and control of gene expression by resveratrol in breast carcinoma cell lines with different metastatic potential. Int J Oncol 1999; 15: 245-252.

    424. Deptala A, Li X, Bedner E, Cheng W, Traganos F, Darzynkiewicz Z. Differences in induction of p53, p21WAF1/CIP1 and apoptosis in relation to cell cycle phase of MCF-7 cells treated with camptothecin. Int J Oncol 1999; 15: 861-871.

    425. Deptala A, Halicka HD, Salomon T, Gorczyca W, Seiter W, Feldman EJ, Traganos F, Darzynkiewicz Z. Malignancy: a new approach to the analysis of apoptosis in the leukemia subpopulations by flow cytometry using a CD45 gating strategy. Hematol 1999; 4:113-121, 1999.

    426. Darzynkiewicz Z, X Li, Bedner E. Detection of DNA strand breakage in analysis of apoptosis and cell proliferation by flow and laser scanning cytometry. Chapter in: Methods in Molecular Biology. Vol 113, "DNA Repair Protocols: Eukaryotic Systems". DS Henderson, Ed. Humana Press., Inc., Totowa, NJ. 1999, pp. 607-619.

    427. Darzynkiewicz Z, Juan G. Selective extraction of fragmented DNA from apoptotic cells for analysis by gel electrophoresis and identification of apoptotic cells by flow cytometry. Chapter in: Methods in Molecular Biology. Vol. 113, "DNA Repair Protocols: Eukaryotic systems". DS Henderson, Ed. Humana Press., Totowa, NJ. 1999, pp. 599-606.

    428. Darzynkiewicz Z, Bedner E, Li X. Analysis of cell death by flow- and laser scanning- cytometry. In: Apoptosis: A Practical Approach. G Studzinski (Ed). Oxford University Press, Oxford, UK. 1999, pp. 57-80

    429. Juan G, Traganos F, Darzynkiewicz Z. Cytometry of the pRB pathway. Chapter in: Cell Cycle: A Practical Approach. G Studzinski (Ed.), Oxford University Press, Oxford, UK. 1999, pp. 145-159.

    2000

    430. Seiter K, Feldman EJ, Halicka HD, Deptala A, Traganos F, Burke HB, Hoang A, Goff H, Pozuollo M, Kancherla R, Darzynkiewicz Z, Ahmed T. Clinical and laboratory evaluation of all-trans retinoic acid modulation of chemotherapy in patients with acute myelogenous leukemia. Br J Hematol 2000; 108:40-47.

    431. Li X, Darzynkiewicz Z. Cleavage of poly(ADP-ribose) polymerase measured in situ in individual cells: relationship to DNA fragmentation and cell cycle position during apoptosis. Exp Cell Res 2000; 255:125-132.

    432. Kajstura J, Pertoldi B, Leri A, Beltrami C-A, Deptala A, Darzynkiewicz Z, Anversa P. Telomere shortening: an in vivo marker of myocyte replication and aging. Am J Pathol 2000; 156:813-819.

    433. Lin C-W, Darzynkiewicz Z, Li X, Traganos F.Bedner E, Tse-Dinh Y-C. Nucleolar localization and cell cycle dependent cleavage of human topoisomerase III" in PHA activated peripheral blood lymphocytes. Exp Cell Res 2000; 256:225-236.

    434. Feldman EJ, Seiter K, Traganos F, Darzynkiewicz Z, Goff H, Pozzuoli M, Baskind P, Santos S, Ahmed, T. Phase II evaluation of a high-dose mitoxantrone based induction regimen in untreated adults with acute myeloid leukemia. Leukemia & Lymphoma 2000; 38:309-315.

    435. Darzynkiewicz Z. Preclinical and clinical development of cyclin-dependent kinase modulators. Correspondence letter. JNCI 2000; 92:1184-1185.

    436. Halicka HD, Murakami T, PapageorgioCN, Mittelman A, Mikulski SM, Shogen K, Darzynkiewicz Z. Induction of differentiation of leukemic (HL-60) or prostate cancer (LNCaP, JCA-1) cells potentiates frequency of apoptosis triggered by onconase. Cell Prolif 2000; 33:407-417.

    437. Bedner E, Ruan Q, Chen S, Kamentsky LA, Darzynkiewicz Z. Multiparameter analysis of progeny of individual cells by laser scanning cytometry. Cytometry 2000; 40:271-279.

    438. Li X, Du L, Darzynkiewicz Z. Caspases are activated during apoptosis independently of dissipation of mitochondrial electrochemical potential. Exp Cell Res 2000; 257:290-297.

    439. Albino AP, Juan G, Traganos F, Reinart L, Connolly J, Rose DP, Darzynkiewicz Z. G1 arrest of melanoma cells by docosahexaenoic acid (omega-3 fatty acid) is associated with decreased pRB phosphorylation. Cancer Res 2000; 60:4130-4145.

    440. DiPietranonio AM, Hsieh T-C, Juan G,Traganos F, Darzynkiewicz Z, Wu J. Fenretinide-induced caspase-3 activity involves increased protein stability in a mechanism distinct from reactive oxygen species elevation. Cancer Res 2000; 60: 4331-4335, 2000.

    441. Darzynkiewicz Z, Traganos F, Chen S. Chinese herbal mixture PC-SPES in treatment of prostate cancer. A review. Int J Oncol 2000; 17:729-736.

    442. Bedner E, Li X, Kunicki J, Darzynkiewicz Z. Translocation of Bax to mitochondria during apoptosis measured by laser scanning cytometry. Cytometry 2000; 41:83-88.

    443. Bedner E, Smolewski P, Amstad P, Darzynkiewicz Z. Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp Cell Res 2000; 259:308-313.

    444. Abdel-Moneim I, Melamed MR, Darzynkiewicz Z, Gorczyca W. Proliferation and apoptosis in solid tumors. Analysis by laser scanning cytometry. Anal Quant Cytol Histol 2000; 22:393-397.

    445. Halicka HD, Bedner E, Darzynkiewicz Z. Segregation of RNA and separate packaging of DNA and RNA in apoptotic bodies during apoptosis. Exp Cell Res, 2000; 260:248-255.

    446. Weisberger J, Wu CD, Liu Z, Wong JYI, Melamed MR, Darzynkiewicz Z, Gorczyca W. Differential diagnosis of malignant lymphomas and related disorders by specific pattern of expression of immunophenotypic markers revealed by multiparameter flow cytometry (Review). Int J Oncol 2000; 17:1165-1177.

    447. Zhang JC, Claffey K, Sakthivel R, Darzynkiewicz Z, Shaw DE, Leal J, Wang YC, Lu FM, MacCrae KR. Two-chain high molecular weight kininogen induces endothelial cell apoptosis and inhibits angiogenesis: partial activity with domain 5. FASEB J 2000; 14: 2589-2600.

    448. Darzynkiewicz Z, Juan G, Bedner E. Determining cell cycle stages by flow cytometry. Section in: Current Protocols in Cell Biology. JS Bonifacino, M Dasso, J Harford, J Lippincott-Schwartz & KM Yamada, Eds. Current Protocols. Wiley-Liss, New York, NY, 2000, pp. 8.4.1-8.4.18,

    449. Darzynkiewicz Z, Bedner E. Analysis of apoptotic cells by flow- and laser scanning- cytometry. In Methods in Enzymology, Vol 322: Methods in Apoptosis Research, JC Reed, (Ed), Academic Press, San Diego, CA, 2000; pp: 18-39.

    450. Darzynkiewicz Z. Cell cycle analysis by flow cytometry. In: Encyclopedia of Life Sciences. Macmillan Reference Ltd, London, 2000 (www.els.net)

    2001

    451. Chen S, Ruan Q, Bedner E, Deptala A, Wang X, Hsieh T-C, Traganos F, Darzynkiewicz Z. Effects of the flavonoid baicalin and its metabolite baicalein on androgen receptor expression, cell cycle progression and apoptosis of prostate cancer cell lines. Cell Prolif 2001; 34: 293-304.

    452. Dobrucki J, Darzynkiewicz Z. Chromatin condensation and sensitivity of DNA in situ to denaturation during cell cycle and apoptosis. A confocal microscopy study. Micron 2001; 32:645-652.

    453. Bedner E, Du L, Traganos F, Darzynkiewicz Z. Caffeine dissociates complexes between DNA and intercalating dyes. Application for bleaching fluorochrome-stained cells for their subsequent re-staining and analysis by laser scanning cytometry. Cytometry 2001; 43:38-45.

    454. Ardelt B, Kunicki J, Traganos F, Darzynkiewicz Z. Chlorophyllin protects cells from the cytostatic and cytotoxic effects of quinacrine mustard but not of nitrogen mustard. Int J Oncol 2001; 18:849-853.

    455. Darzynkiewicz Z, Bedner E, Smolewski P. Flow cytometry in analysis of cell cycle and apoptosis. Semin Hematol 2001; 38:179-193.

    456. Smolewski P, Bedner E, Du L, Hsieh T-C, Wu JM, Phelps DJ, Darzynkiewicz Z. Detection of caspases activation by fluorochrome-labeled inhibitors: multiparameter analysis by laser scanning cytometry, Cytometry 2001; 44:73-82.

    457. Deptala A, Bedner E, Darzynkiewicz Z. Unique analytical capabilities of laser scanning cytometry that complement flow cytometry. Folia Histochem Cytobiol 2001; 39: 87-89.

    458. Darzynkiewicz Z, Bedner E, Smolewski P. New approaches in analysis of apoptosis by cytometry. Acta Haematol Pol 2001; 32 (Suppl 1):77-79.

    459. Darzynkiewicz Z, Li X, Bedner E. Use of flow and laser scanning cytometry in analysis of cell death. Chapter in: Methods Cell Biol 2001; 66: 69-109.

    460. Smolewski P, Bedner E, Gorczyca W, Darzynkiewicz Z. Liquidless cell staining by dye diffusion from gels and analysis by laser scanning cytometry: potential application at microgravity conditions in space. Cytometry 2001; 44:355-360..

    461. Leri A, Berlucchi I, Limana F, Deptala A, Darzynkiewicz Z, Hintze TH, Kajstura J, Nadal-Ginard B, Anversa P. Telomerase expression and activity are coupled with myocyte proliferation and preservation of telomeric length in the failing heart. Proc Natl Acad Sci USA 2001; 98:8626-8631.

    462. Smolewski P, Grabarek J, Kamentsky LA., Darzynkiewicz Z. Bivariate analysis of cellular DNA vs RNA content by laser scanning cytometry using the product of signal subtraction (Differential Fluorescence; DF) as a separate parameter. Cytometry 2001; 45:73-78.

    463. Smolewski P, Ruan Q, Vellon L, Darzynkiewicz Z. The micronuclei assay by laser scanning cytometry. Cytometry 2001; 45:19-26.

    464. Smolewski P, Grabarek J, Phelps DJ, Darzynkiewicz Z. Stathmo-apoptosis: arresting apoptosis by fluorochrome-labeled inhibitor of caspases. Int J Oncol 2001; 19:657-663.

    465. Darzynkiewicz Z, Smolewski P, Bedner E. Use of flow- and laser scanning- cytometry to study mechanisms regulating cell cycle and controlling cell death. Clinics Lab Med 2001; 21:857-873.

    466. Gorczyca W, Smolewski P, Grabarek J, Ardelt B, Ita M, Melamed MR, Darzynkiewicz Z. Morphometry of nucleoli and expression of nucleolin analyzed by laser scanning cytometry in mitogenically stimulated lymphocytes. Cytometry 2001; 45:206-213.

    467. Du L, Smolewski P, Bedner E, Traganos F, Darzynkiewicz Z. Selective protection of mitogenically stimulated human lymphocytes but not leukemic cells from cytosine arabinoside-induced apoptosis by LY294002, a phosphoinositol-3 kinase inhibitor. Int J Oncol 2001; 19:811-820.

    468. Traganos F, Juan G, Darzynkiewicz Z. Cell cycle analysis of drug treated cells. Meth Molec Biol 2001; 95: 229-240

    469. Darzynkiewicz Z, Juan G, Traganos, F. Assaying drug induced apoptosis. Meth Molec Biol 2001; 95:241-254.

    470. Juan G, Darzynkiewicz Z. The detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color. Chapter in: Apoptosis and Programmed Cell Death Protocols. G. Cowling, Ed. Molecular Medicine Series. Humana Press, Totowa, NJ. 2001

    471. Gorczyca W, Deptala A, Bedner E, Li X, Melamed MR, Darzynkiewicz Z. Analysis of human tumors by laser scanning cytometry. Meth Cell Biol 2001; 64:421-443

    472. Darzynkiewicz Z, Bedner E, Traganos F. Difficulties and pitfalls in analysis of apoptosis. Chapter in: Methods Cell Biol 2001; 63:527-559.

    473. Juan G, Traganos F, Darzynkiewicz Z. Methods to identify mitotic cells by flow cytometry. Chapter in: Methods Cell Biol 2001; 63:343-354.

    474. Darzynkiewicz Z, Li X, Bedner E. Use of flow- and laser scanning- cytometry in analysis of cell death. Chapter in: Methods Cell Biol 2001; 66:69-109.

    475. Darzynkiewicz Z, Juan G, Bedner E. Determining cell cycle stages by flow cytometry. In: Curr Protocols Cell Biol. 2001; Chapter 8, Unit 8.4.

    476. Smolewski P, Bedner E, Gorczyca W, Darzynkiewicz Z. Fluorescence resonance energy transfer analysis of cell surface receptor interactions and signaling using spectral variants of the green fluorescent protein. Commun Clin Cytometry 2001; 46:361-368.

    2002

    477. Moog-Lutz C, Guibal FC, Cave-Riant F, Smolewski P, Di Giois Y, Darzynkiewicz Z, Lutz PG, Cayre YE. ASB-2 inhibits growth and promotes commitment in myeloid leukemia cells J Biol Chem 2002; 277:218-224.

    478. Darzynkiewicz Z. To cycle or not to cycle ? The role of checkpoints in maintenance of genome integrity and cancer. Cancer Biol Ther 2002; 1:76-77.

    479. Grabarek J, Dragan M, Lee BW, Johnson GL, Darzynkiewicz Z. Activation of chymotrypsin-like serine protease(s) during apoptosis detected by affinity-labeling of the enzymatic center with the fluoresceinated inhibitor. Int J Oncol 2002; 20: 225-233.

    480. Smolewski P, Grabarek J, Lee BW, Johnson GL, Darzynkiewicz Z. Kinetics of HL-60 cell entry to apoptosis during treatment with TNF-? or camptothecin assayed by the stathmo-apoptosis method. Cytometry 2002; 47:143-149.

    481. Darzynkiewicz Z, Smolewski P, Bedner E. Use of flow and laser scanning cytometry to study mechanisms regulating cell cycle and controlling cell death. Hematology/Oncology Clinics of North America 2002; 16:1-17.

    482. Hsieh TC, Lu X, Guo J, Xiong W, Kunicki J, Darzynkiewicz Z, Wu JM. Effects of herbal preparation Equiguard on hormone-responsive and hormone-refractive prostate carcinoma cells: mechanistic studies. Int J Oncol 2002; 20:681-689.

    483. Tsai SY, Hsieh T-C, Ardelt B, Darzynkiewicz Z, Wu JM. Combined effects of onconase and IFN-beta on proliferation, macromolecular synthesis and expression of STAT- in JCA-1 cells. Int J Oncol 2002; 20:891-896.

    484. Kapuscinski J, Ardelt B, Piosik J, Zdunek M, Darzynkiewicz Z. The modulation of the DNA-damaging effect of polycyclic aromatic agents by xanthines. Part I. Reduction of cytostatic effects of quinacrine mustard by caffeine. Biochem Pharmacol 2002; 63:625-634.

    485. Wang Q, Xie S, Chen J, Fukasawa K Naik U, Traganos F, Darzynkiewicz Z, Jhanwar-Uniyal M, Dai W. Cell cycle arrest and apoptosis induced by human polo-like kinase 3 is mediated through perturbation of microtubule integrity. Molec Cell Biol 2002; 22:3450-3459.

    486. Grabarek J, Darzynkiewicz Z. Versatility of analytical capabilities of laser scanning cytometry. Clin Appl Immunol Rev (CAIR) 2002; 2:75-92.

    487. Darzynkiewicz Z, Bedner E, Smolewski P. In situ detection of DNA strand breaks in analysis of apoptosis by flow- and laser scanning- cytometry. Meth Molec Biol 2002; 203: 69-77.

    488. Darzynkiewicz Z, Bedner E, Smolewski P, Lee BW, Johnson GL. Detection of caspases activation in situ by fluorochrome-labeled inhibitors of caspases (FLICA). Meth Molec Biol 2002; 203:289-299.

    489. Smolewski P, Grabarek J, Halicka HD, Darzynkiewicz Z. Assay of caspases activation in situ combined with probing plasma membrane integrity detects three distinct stages of apoptosis. J Immunol Meth 2002; 265:111-121.

    490. Gorczyca W, Weisberger J, Liu Z, Tsang P, Hossein M, Wu CD, Dong H, Wong JYL, Tugulea S, Dee S, Melamed MR, Darzynkiewicz Z. An approach to diagnosis of T-cell lympho-proliferative disorders by flow cytometry. Cytometry (Clinical Cytometry) 2002; 50:177-190.

    491. Darzynkiewicz Z, Bedner E, Smolewski P. Analysis of apoptosis by laser scanning cytometry. Chapter in: Meth Pharmacol Toxicol, Apoptosis Methods in Pharmacology and Toxicology. MA Davis Ed., Humana Press (Totowa, NJ) 2002; 37-58.

    492. Grabarek J, Du L, Johnson GL, Lee B, Phelps DJ, Darzynkiewicz Z. Sequential activation caspases and serine proteases (serpases) during apoptosis. Cell Cycle 2002; 1:124-131.

    493. Grabarek J, Amstad P, Darzynkiewicz Z. Use of fluorescently labeled inhibitors of caspases as affinity labels to detect activated caspases. Human Cell (Jap) 2002; 15: 1-12.

    494. Grabarek J, Ardelt B, Du L, Darzynkiewicz Z. Activation of caspases and serine proteases during apoptosis induced by onconase (Ranpirnase). Exp Cell Res 2002; 278:61-71.

    495. Hsieh T-C, Xiong W, Traganos F, Darzynkiewicz Z, Wu JM. Effects of PC-SPES on proliferation and expression of AR/PSA in androgen responsive LNCaP cells are independent of estradiol. Anticancer Res 2002; 22:2051-2060.

    496. Grabarek J, Darzynkiewicz Z. In situ activation of caspases and serine proteases during apoptosis detected by affinity labeling their enzyme active centers with fluorochrome-tagged inhibitors. Exp Hematol 2002; 30:982-992.

    497. Halicka HD, Smolewski P, Darzynkiewicz Z, Dai W, Traganos F. Arsenic trioxide arrests cells early in mitosis leading to apoptosis.Cell Cycle 2002; 1:201-209.

    498. Blagosklonny MV, Darzynkiewicz Z, Figg WD. Flavopiridol inversely affects p21WAF1/CIP1 and p53 and protects p21-sensitive cells from paclitaxel. Canc Biol Ther. 2002; 1: 424-430.

    499. Xie S, Wang Q, Luo L, Ruan Q, Liu T, Jhanhwar-Uniyal M, Darzynkiewicz Z, Traganos F, Dai W. Proteasome-dependent down-regulation of p21WAF1/CIP1 induced by reactive oxygen species. J Interferon & Cytokine Res 2002; 22: 957-963, 2002.

    500. Hsieh T-C, Halicka D, Lu X, Kunicki J, Guo J, Darzynkiewicz Z, Wu JM. Effects of resveratrol on the G0 to G1 transition and cell cycle progression of mitogenically stimulated human lymphocytes. Biochem Biophys Res Commun 2002; 297:1311-1317..

    501. Teodori L, Grabarek J, Smolewski P, Ghibelli L, Bergamaschi A, De Nicola M, Darzynkiewicz Z. Exposure of cells to static magnetic field accelerates loss of integrity of plasma membrane during apoptosis.Cytometry 2002; 49:113-119.

    502. Grabarek J, Ardelt B, Kunicki J, Darzynkiewicz Z. Detection of in situ activation of transglutaminase during apoptosis: correlation with the cell cycle phase by multiparameter flow- and laser scanning- cytometry. Cytometry 2002; 49:83-89.

    503. Blagosklonny MV, Darzynkiewicz Z. Four birds with one stone: RAPA as potential anticancer therapy. Canc Biol Ther 2002; 1:363-365.

    504. Blagosklonny MV, Robey R, Sackett DL, Du L, Traganos F, Darzynkiewicz Z, Fojo T, Bates SE. Histone deacetylase inhibitors all induce p21 but differentialy cause tubulin acetylation, mitotic arrest, and cytotoxicity. Molec Canc Ther 2002; 1:937-941.

    505. Halicka DH, Pozarowski P, Ita M, Ardelt W, Mikulski SM, Shogen K, Darzynkiewicz Z. Enhancement of activation-induced apoptosis of lymphocytes by the cytotoxic ribonuclease onconase (Ranpirnase). Int J Oncol 2002; 21:1245-1250.

    506. Hsieh T-C, Kunicki J, Darzynkiewicz Z, Wu MJ. Effects of extract of Coloris versicolor (I'm-YunityTM ) on cell cycle progression and expression of interleukins 1-?, -6, and -8 in promyelocytic HL-60 leukemic cells and mitogenically stimulated human lymphocytes. J Altern Compl Med 2002; 8:591-602.

    507. Darzynkiewicz Z. One more reason to get arrested (in G1).Cell Cycle 2002; 1:318-319.

    508. Blagosklonny MV, Darzynkiewicz Z. Cyclotherapy: Protecting of normal cells and unshielding of cancer cells. Cell Cycle 2002; 1:375-382.

    509. Grabarek J, Darzynkiewicz Z. In situ activation of caspases revealed by affinity labeling their enzymatic sites with fluorochrome-labeled inhibitors. Chapter in: Caspases. M Los & H Walczak, Eds, Landes/ Eurekah.com and Kluwer Academic /Plenum Publishers., New York, NY. 2002; pp 197-209.

    510. Grabarek J, Darzynkiewicz, Z. Flow Cytometry of Apoptosis. Section in: Current Protocols in Cytometry. P Robinson, Z Darzynkiewicz, P Dean, A Orfao, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, N.Y.2002. Section 7. 19. pp. 7.19.1-7.9.33.

    511. Smolewski P, Szmigielska A, Cebula B, Sobczak A, Darzynkiewicz Z, Robak T. Pro-apoptotic effect of rituximab alone or in combination with purine nucleoside analagues on B-CLL chronic lymphocytic leukemia cells measured by fluorochrome labeled inhibitors of caspases (FLICA) assay. Blood 2002; 11:387A.

    2003

    512. Strife A, Wisniewski D, Chongyuan L, Lambek CL, Darzynkiewicz Z, Silver RT, Clarkson B. Direct evidence that Bcr-Abl tyrosine kinase activity disrupts normal synergistic interactions between Kit ligand and cytokines in primary primitive progenitor cells. Molec Canc Res 2003; 1: 176-185.

    513. Haider AS, Grabarek J, Eng B, Pedraza P, Ferreri NR, Balazs EA, Darzynkiewicz Z. In vitro wound healing analyzed by laser scanning cytometry. Accelerated healing of epithelial cell monolayers in the presence of hyaluronate. Cytometry A 2003; 53A:1-8.

    514. Ardelt B, Ardelt W, Darzynkiewicz Z. Cytotoxic ribonucleases and RNA interference (RNAi). Cell Cycle 2003; 2:22-24.

    515. Smolewski P, Darzynkiewicz Z, Robak T. Caspase-mediated cell death in hematological malignancies: Theoretical considerations, methods of assessment, and clinical implications. Leukemia & Lymphoma 2003; 44;1089-1104.

    516. Blagosklonny MV, Darzynkiewicz Z. Why Iressa failed: toward novel use of kinase inhibitors. Canc Biol & Ther 2003; 2:137-140.

    517. Pozarowski P, Halicka DH, Darzynkiewicz Z. NF-?B inhibitor sesquiterpene parthenolide nduces concurrently atypical apoptosis and necrosis: Difficulties in identification of dead cells in such cultures. Cytometry A 2003; 54A:118-124.

    518. Smolewski P, Darzynkiewicz Z. [Current methods for measurement apoptosis] (in Polish). Acta Haematol Pol 2003; 34:35-47.

    519. Pozarowski P, Halicka DH, Darzynkiewicz Z. Cell cycle effects and caspase-dependent and independent death of HL-60 and Jurkat cells treated with the NF-?B inhibitor parthenolide. Cell Cycle 2003; 2:377-383.

    520. Pietrzak M, Wieczorek Z, Stachelska A, Darzynkiewicz Z. Interactions of chlorophyllin with acridine orange, quinacrine mustard and doxorubicin analyzed by light absorption and fluorescence spectroscopy. Biophys Chem 2003; 104:305-313.

    521. Aoe K, Hiraki A, Murakami T, Murakami K, Makihata K, Takao K, Eda R, Maeda T, Sugi K, Darzynkiewicz Z, Takeyama H. Relative abundance and patterns of correlation among six cytokines in pleural fluid measured by cytometric bead array. Int J Molec Med 2003; 12:193-198.

    522. Pozarowski P, Huang X, Halicka DH, Lee B, Johnson G, Darzynkiewicz Z. Interactions of fluorochrome-labeled caspase inhibitors with apoptotic cells. A caution in data interpretation. Cytometry A, 2003; 55A:50-60.

    523. Lee BW, Johnson GL, Hed SA, Darzynkiewicz Z, Talhouk JW, Mehrota S. DEVDase detection in intact apoptotic cells using the cell permeant fluorogenic substrate, (z-DEVD)2-cresyl violet. BioTechniques 2003; 35:1080-1085.

    524. Huang X, Traganos F, Darzynkiewicz Z. DNA damage induced by DNA topoisomerase I- and topoisomerase II- inhibitors detected by histone H2AX phosphorylation in relation to the cell cycle phase and apoptosis. Cell Cycle 2003; 2:614-619.

    525. Abraham NG, Kushida T, McClung J, Quan S, Lafaro R, Darzynkiewicz Z, Wolin M. Heme oxygenase-1 attenuates glucose-mediated cell growth arrest and apoptosis in human microvessel endothelial cells. Circulation Research 2003; 93:507-514, 2003.

    526. Haider A, Lee I, Grabarek J, Darzynkiewicz Z, Ferreri NR. Dual functionality of cyclooxygenase-2 as a regulator of tumor necrosis factor-mediated G1 shortening and nitric oxide-mediated inhibition of vascular smooth muscle cell proliferation.Circulation 2003; 108:1015-1021.

    527. Darzynkiewicz Z, Juan G, Traganos F. Cytometry of cell cycle regulatory proteins. Chapter in: Progress in Cell Cycle Research 2003; 5:533-542 (Meijer L, Jezequel A, & Roberge M, Eds.) Roscoff, France.

    2004

    528. Darzynkiewicz Z, Huang X. Analysis of DNA content by flow cytometry. Chapter in: Current Protocols in Immunology. R Coico, Ed. Current Protocols/Wiley-Liss, New York, NY. 2004 Chapter 5, Unit 5:7

    529. Juan G, Darzynkiewicz Z. Detection of mitotic cells by flow cytometry. In Current Protocols in Cytometry. P Robinson, Z Darzynkiewicz, P Dean, A Orfao, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, NY.2004, pp: 7.24.1 7.24.6

    530. Pozarowski P, Huang X, Gong RW, Priebe W, Darzynkiewicz Z. Simple, semi-automatic assay of cytostatic and cytotoxic effects of antitumor drugs by laser-scanning cytometry (LSC): Effects of the bis-intercalator WP631 on growth and cell cycle of T-24 cells.Cytometry A 2004; 57A: 113-119.

    531. Wang Q, Tongyi Liu T, Fang Y, Xie S, Huang X, Mahmood R, Ramasiwamy M, Sakamoto KM, Darzynkiewicz Z, Xu M, Dai W. BUBR-1 deficiency results in abnormal megakaryopoiesis. Blood 2004; 103:1278-1285.

    532. Smolewska E, Brozik H, Smolewski P, Darzynkiewicz Z, Stanczyk J. Regulation of peripheral blood and synovial fluid lymphocyte apoptosis in juvenile idiopathic arthritis. Scand J Rheumatol 2004; 33:1-6.

    533. Ruan Q, Wang Q, Xie S, Fang Y, Darzynkiewicz Z, Guan K, Jhanwar-Uniyal M, Dai W. Polo-like kinase 3 is Golgi localized and involved in regulating Golgi fragmentation during the cell cycle. Exp Cell Res 2004; 294:51-59.

    534. Darzynkiewicz Z, Crissman HA, Jacobberger JW. Cytometry of the cell cycle. Cycling through history. Cytometry 2004; 58A;21-32.

    535. Huang X, Okafuji M, Traganos F, Luther E, Holden E, Darzynkiewicz Z. Assessment of histone H2AX phosphorylation induced by DNA topoisomerase I and II inhibitors topotecan and mitoxantrone and by DNA crosslinking agent cisplatin.Cytometry A 2004; 58A:99-110.

    536. Huang X, Ruan Q, Fang Y, Traganos F, Darzynkiewicz Z, Dai W. Physical and functional interactions between mitotic kinases during polyploidization and megakaryocytic differentiation. Cell Cycle 2004; 3:946-951

    537. Albino AP, Huang X, Yang J, GietlD, Jorgensen E, Traganos F, Darzynkiewicz Z. Induction of histone H2AX phosphorylation in A549 human pulmonary epithelial cells by tobacco smoke and in human bronchial epithelial cells by smoke condensate: A new assay to detect the presence of potential carcinogens in tobacco. Cell Cycle 2004; 3:1062-1068.

    538. Blagosklonny VM, Darzynkiewicz Z, Halicka HD, Pozarowski P, Demidenko Z, Barry JJ, Kamath KR, Herrman RA. Paclitaxel induces primary and postmitotic G1 arrest in human arterial smooth muscle cells. Cell Cycle 2004; 3:1050-1056.

    539. Jamroziak K, Smolewski P, Cebula B, Szmigielska-Kaplon A, Darzynkiewicz Z, Robak T. Relation of P-glycoprotein expression with spontaneous in vitro apoptosis in B-cell chronic lymphocytic leukemia. Neoplasma 2004; 51:181-187.

    540. Fu C, Hsieh T-C, Guo J, Kunicki J, Lee MYWT, Darzynkiewicz Z, Wu JM. Licochalcone-A, a novel flavonoid, causes G2 and late-G1 arrests in PC-3 cells. Biochem Biphys Res Commun 2004; 322:263-270.

    541. Hiraki A, Fuji N, Murakami T, Kiura K, Aoe K, Yamane H, Masuda K, Maeda T, Sugi K, Darzynkiewicz Z, Tanimoto M, Harada M. High frequency of allele-specific down-regulation of HLA class I expression in lung cancer lines. Anticancer Res 2004; 24:1525-1528.

    542. Huang X, King M, Halicka DH, Traganos F, Okafuji M, Darzynkiewicz Z. Histone H2AX phosphorylation induced by selective photolysis of BrdU-labeled DNA with UV light: relationship to cell cycle phase. Cytometry A 2004; 62A:1-7, 2004

    543. King MA, Halicka HD, Darzynkiewicz Z. Pro- and anti- apoptotic effects of an inhibitor of chymotrypsin-like serine proteases. Cell Cycle 2004; 3:1566-1571

    544. Tsai SY, Ardelt B, Hsieh T-C, Darzynkiewicz Z, Wu JM. Treatment of Jurkat acute T-lymphocytic leukemia cells by onconase is accompanied by an altered nucleocytoplasmic distribution and reduced expression of transcription factor NF-kB. Int J Oncol 2004; 25:1745-1752.

    545. Pozarowski P, Darzynkiewicz Z. Analysis of cell cycle by flow cytometry: Meth Molec Biol 2004; 281:301-311.

    546. Roederer M, Darzynkiewicz Z, Parks DR. Guidelines for the presentation of flow cytometric data. Methods Cell Biol 2004; 75:241-257.

    547. Darzynkiewicz Z, Huang X, Okafuji M, King MA. Cytometric methods to detect apoptosis. Methods Cell Biology 2004; 75:307-342.

    548. Pozarowski P, Grabarek J, Darzynkiewicz Z. Flow cytometry of apoptosis. In: Curr Protocols in Cell Biol. 2004 Feb; Chapter 18, Unit 18.8.

    2005

    549. Chen S, Gao J, Halicka HD, Huang X, Traganos F, Darzynkiewicz Z. The cytostatic and cytotoxic effects of oridonin (Rubescenin), a diterpenoid from Rabdosia Rubescens on tumor cells of different lineage. Int J Oncol 2005; 26:579-588.

    550. Gupte S R, Pozarowski P, Grabarek J, Traganos F, Darzynkiewicz Z, Lee MYWT. RI? influences cell proliferation in cancer cells by transporting RFC40 into the nucleus. Cancer Biol & Ther 2005; 4:429-437.

    551. Demidenko ZN, Halicka D, Kunicki J, McCubrey JA, Darzynkiewicz Z, Blagosklonny MV. Selective killing of adriamycin-resistant (G2 Checkpoint-deficient and MRP-1-expressing) cancer cells by docetaxel. Cancer Res 2005; 65:4401-4407.

    552. Pozarowski P, Holden E, Darzynkiewicz Z. Laser scanning cytometry. Principles and applications. Meth Molec Biol 2005; 319:165-192.

    553. Huang X, Lee B, Johnson G, Naleway J, Guzikowski A, Dai W, Darzynkiewicz Z. Novel assay utilizing fluorochrome-tagged physostigmine (Ph-F) to in situ detect active acetylcholinesterase (AChE) induced during apoptosis.Cell Cycle 2005; 4:140-147.

    554. Huang HS, Pozarowski P, GaoY, Darzynkiewicz Z, Lee EY. Protein phosphatase-1 inhibitor-3 is co-localized to the nucleoli and centrosomes with PP1gamma1 and PP1alpha, respectively. Arch Biochem Biophys 2005; 443:33-44.

    555. Halicka HD, Huang X, Traganos F, King MA, Dai W, Darzynkiewicz Z. Histone H2AX phosphorylation after cell irradiation with UV-B: Relationship to cell cycle phase and induction of apoptosis.Cell Cycle 2005; 4:339-345.

    556. Friedrich TD, Bedner E, Darzynkiewicz Z, Lehman JM. Rereplication of host cell DNA without reloading MCM proteins in Simian virus 40 lytic infection.Cytometry A, 2005; 68A:10-18.

    557. Huang X, Dai W, Darzynkiewicz Z. Enforced adhesion of hematopoietic cells to culture dish induces endomitosis and polyploidy.Cell Cycle 2005; 4:801-505.

    558. Huang X, Halicka HD, Traganos F, Tanaka T, Kurose A, Darzynkiewicz Z. Cytometric assessment of DNA damage in relation to cell cycle phase and apoptosis.Cell Prolif 2005; 38: 223-243.

    559. Kurose A, Tanaka T, Huang X, Halicka HD, Traganos F, Dai W, Darzynkiewicz Z. Assessment of ATM phosphorylation on Ser-1981 induced by DNA topoisomerase I and II inhibitors in relation to Ser-139-histone H2AX phosphorylation, cell cycle phase and apoptosis. Cytometry A 2005; 68A:1-9.

    560. Jain K, Roy U, Ardelt B, Krishn, UM, Falck JR, Pozarowski P, Kunicki J, Darzynkiewicz Z, Balazy M. 5E, 8Z, 14Z-eicosatetraenoic acid, a novel trans isomer of arachidonic acid, causes G1 phase arrest and induces apoptosis of HL-60 cells. Int J Oncol 2005; 27:1177-1185.

    561. Blagosklonny M, Darzynkiewicz Z. Strategies of protection of normal cells during chemo- and radio- therapy based on modulation of cell cycle and apoptotic pathways. Chapter in: Apoptotic pathways as target for novel therapies of cancer and other diseases. M Los & SB Gibson, Eds. Springer, New York, NY, ISBN: 0-387-23384-9, 2005, pp. 357-376.

    562. Huang X, Halicka HD, Darzynkiewicz Z. Detection of histone H2AX phosphorylation on Ser-139 as an indicator of DNA damage (DNA double-strand breaks). Section in: Current Protocols in Cytometry. P Robinson, Z Darzynkiewicz, P Dean, A Orfao, P Rabinovitch, C Stewart, H Tanke & L Wheeless, Eds. Current Protocols/Wiley-Liss, New York, NY, 2005, pp. 7.27.1-7.27.7.

    2006

    563. Chen J, Huang X, Halicka D, Brodsky S, Avram A, Eskander J, Bloomgarden NA, Darzynkiewicz Z, Goligorsky MS. Contribution of p16INK4a and p21CIP1 pathways to induction of premature senescence of human endothelial cells: Permissive role of p53. Am J Physiol Heart Circ Physiol 2006; 290:H1575-1586.

    564. Tanaka T, Kurose A, Huang X, Dai W, Darzynkiewicz, Z. ATM kinase activation and histone H2AX phosphorylation as indicators of DNA damage by DNA topoisomerase I inhibitor topotecan and during apoptosis.Cell Prolif 2006; 39:49-60.

    565. Kurose A, Tanaka T, Huang X, Traganos F, Dai W, Darzynkiewicz Z. Effects of hydroxyurea and aphidicolin on phosphorylation of ATM on Ser 1981 and histone H2AX on Ser 139 in relation to cell cycle phase and induction of apoptosis. Cytometry A 2006; 69A:212-221.

    566. Albino AP, Huang X, Jorgensen E, Gietl D, Traganos F, Darzynkiewicz Z. Induction of DNA double-strand breaks in A549 and normal human pulmonary epithelial cells by cigarette smoke is mediated by free radicals. Int J Oncol 2006; 28:1491-1505.

    568. Gupte RS, Sampson V, Traganos F, Darzynkiewicz Z, Lee M Y W T. Cyclic AMP regulates the expression and nuclear translocation of RFC40 in MCF7 Cells. Exp Cell Res 2006; 212:976-806.

    569. Fang Y, Li T, Wang X, Yang Y-M, Deng H, Kunicki J, Traganos F, Darzynkiewicz Z, Lu L, Dai W. BubR1 is involved in regulation of DNA damage responses. Oncogene 2006; 25:3598-3605.

    570. Huang X, Kurose A, Tanaka T, Traganos F, Dai W, Darzynkiewicz Z. Sequential phosphorylation of Ser-10 on histone H3 and Ser-139 on histone H2AX and ATM activation during premature chromosome condensation: Relationship to cell-cycle and apoptosis. Cytometry A 2006; 69A:222-229.

    571. Fuji N, Hiraki A, Aoe K, Murakami T, Ikeda K, Masuda K, Matsuo K, Shinagawa K, Ishimaru F, Sugi K, Darzynkiewicz Z, Tanimoto M. Serum cytokine concentrations and acute graft-versus-host disease after allogeneic peripheral blood stems cell tranmsplantation: Concurrent measurement of ten cytokines and their respective ratios using cytometric bead assay. Int J Mol Med 2006; 17:881-885.

    572. Gupte RS, Traganos F, Darzynkiewicz Z, Lee YWT. Phosphorylation of RIa by cyclin-dependent kinase CDK 2/Cyclin E modulates the dissociation of the RIa-RFC40 complex. Cell Cycle 2006; 5:653-660.

    573. Pietrzak M, Wieczorek Z, Wieczorek J, Darzynkiewicz Z. The interceptor properties of chlorophyllin measured spectroscopically within the three-component system: intercalator-DNA-chlorophyllin. Biophys J 2006; 123:11-19.

    574. Huang X, Tanaka T, Kurose A, Traganos F, Darzynkiewicz Z. Constitutive histone H2AX phosphorylation on Ser-139 in cells untreated by genotoxic agents is cell-cycle phase specific and attenuated by scavenging reactive oxygen species. Int J Oncol 2006; 29:495-501.

    575. Kurose A, Tanaka T, Huang X, Traganos F, Darzynkiewicz Z. Synchronization in the cell cycle by inhibitors of DNA replication induces histone H2AX phosphorylation, an indication of DNA damage. Cell Prolif 2006; 39:231-240.

    576. Jiang N, Wang X Jhanwar-Uniyal M, Darzynkiewicz Z, Dai W. Polo-box domain of PLK3 functions as a centrosome localization signal, over-expression of which causes mitotic arrest, cytokinesis defects, and apoptosis. J Biol Chem 2006; 281:10577-82.

    577. Tanaka T, Kurose A, Halicka HD, Traganos F, Darzynkiewicz Z. 2-Deoxy-D-glucose reduces the level of constitutive activation of ATM and phosphorylation of histone H2AX. Cell Cycle 2006; 5: 878-882.

    578. Tanaka T, Kurose A, Huang X, Traganos F, Dai W, Darzynkiewicz Z. Extent of constitutive histone H2AX phosphorylation on Ser-139 varies in cells with different TP53 status. Cell Prolif 2006; 39:313-323.

    579. Huang X, Kurose A, Tanaka T, Traganos F, Dai W, Darzynkiewicz Z. Activation of ATM and histone H2AX phosphorylation induced by mitoxantrone but not by topotecan is prevented by the antioxidant N-acetyl-L-cysteine Cancer Biol & Ther 2006; 5:959-964.

    580. Mandecki W, Ardelt B, Corradetti T, Davidovitz H, Flint J, Huang X, Kopacka W, Lin X, Darzynkiewicz Z. Microtransponders, the miniature radiofrequency-identifiable (RFID) electronic chips, as platforms for cell growth in cytotoxicity assays. Cytometry A 2006; 69A: 1097-1105.

    581. Tanaka T, Kurose A, Halicka HD, Huang X, Traganos F, Darzynkiewicz Z. Nitrogen oxide-releasing aspirin induces histone H2AX phosphorylation, ATM activation, and apoptosis preferentially in S-phase cells; involvement of reactive oxygen species. Cell Cycle 2006; 5: 1669-1674.

    582. Tanaka T, Halicka HD, Huang X, Traganos F, Darzynkiewicz Z. Constitutive histone H2AX phosphorylation and ATM activation, the reporters of DNA damage by endogenous oxidants. Cell Cycle 2006; 5:1940-1945.

    583. Tanaka T, Halicka HD, Traganos F, Darzynkiewicz Z. Phosphorylation of histone H2AX on Ser 139 and activation of ATM during oxidative burst in phorbol ester-treated human leukocytes. Cell Cycle 2006; 5:2671-2675.

    584. Huang X, Darzynkiewicz Z. Cytometric assessment of histone H2AX phosphorylation, a reporter of DNA damage. Meth Molec Biol 2006; 314:73-80.

    585. Darzynkiewicz Z, Huang X, Okafuji M. Detection of DNA strand breaks by flow and laser scanning cytometry in studies of apoptosis and cell proliferation (DNA replication). Meth Molec Biol 314; 81-94, 2006.

    586. Pozarowski P, Holden E, Darzynkiewicz Z. Laser scanning cytometry: Principles and applications. Meth Molec Biol. 2006; 319:165-192.

    587. Darzynkiewicz Z, Pozarowski P, Juan G. Cell cycle analysis by flow and laser scanning cytometry. Chapter in: Cell Biology. A Laboratory Handbook. Third Edition. JE Celis, Ed,. Elsevier/Academic Press, San Diego, CA. 2006, pp. 279-289.

    588. Smolewski P, Cebula B, Jamroziak K, Janus A, Blagosklonny MV, Darzynkiewicz Z, Robak T. Rapamycin renders acute lymphoblastic leukemia cells susceptible to apoptosis induced by cladribine while protecting normal lymphocytes: a rationale for the cyclotherapy concept. Acta Haematol Polon 2006; 37: 553-566.

    2007

    589. Lin JH, Takano T, Arcuino G, Wang X, Hu F, Darzynkiewicz Z, Nunes M, Goldman, SA, Nedergaard M. Purinergic signaling regulates neural progenitor cell expansion and neurogenesis. Dev Biol 2007; 302:356-366.

    590. Tanaka T, Kajstura M, Halicka HD, Traganos F, Darzynkiewicz Z. Constitutive histone H2AX phosphorylation and ATM activation are strongly amplified during mitogenic stimulation of lymphocytes. Cell Prolif 2007; 40:1-13.

    591. Kajstura M, Halicka HD, Pryjma J, Darzynkiewicz Z. Discontinuous fragmentation of nuclear DNA during apoptosis revealed by discrete sub-G1 peaks on DNA content histograms. Cytometry A 2007; 71A:125-131.

    592. Tanaka T, Halicka HD, Traganos F, Seiter K, Darzynkiewicz Z. Induction of ATM activation, histone H2AX phosphorylation and apoptosis by etoposide: relation to the cell cycle phase. Cell Cycle 2007; 6:371-376.

    593. Halicka HD, Ardelt B, Shogen K, Darzynkiewicz Z. Mild hyperthermia predisposes tumor cells to undergo apoptosis upon treatment with onconase. Int J Oncol 2007; 30:841-847.

    594. Darzynkiewicz Z, Pozarowski, P. All that glitters is not gold: All that binds FLICA is not caspase. A caution in data interpretation - and new opportunities.Cytometry A 2007; 71A:536-537.

    595. Tanaka T, Huang X, Halicka HD, Zhao H, Traganos F, Albino AP, Dai W, Darzynkiewicz Z. Cytometry of ATM activation and histone H2AX phosphorylation to estimate extent of DNA damage induced by exogenous agents. Cytometry A 2007; 71A:648-661.

    596. Schulenburg C, Ardelt B, Ardelt, Arnold U, Shogen K, Ulbrich-Hofmann R.Darzynkiewicz Z. The interdependence between catalytic activity, conformational stability, and cytotoxicity of Onconase. Cancer Biol & Ther 2007; 6:233-1239.

    597. Tanaka T, Huang X, Jorgensen E, Gietl E, Traganos F, Darzynkiewicz Z, Albino AP. ATM activation accompanies histone H2AX phosphorylation in A549 cells upon exposure to tobacco smoke. BMC Cell Biol 2007. Epub June 26, 8:26.

    598. Ardelt B, Juan G, Burfeind P, Salomon, T, Wu JM, Hsieh T-C, Li X, Sperry R, Pozarowski P, Shogen K, Ardelt W, Darzynkiewicz Z. Onconase, an anti-tumor ribonuclease suppresses oxidative stress. Int J Oncol 2007; 31:663-669.

    599. Tadi K, Ashok BT, Chen Y, Banerjee D, Wysocka-Skrzela B, Konopa J, Darzynkiewicz Z, Tiwari RK.Pre-clinical evaluation of 1-nitroacridine derived chemotherapeutic agent that has preferential cytotoxic activity towards prostate cancer. Cancer Biol Ther 2007; 6:632-1637.

    600. Zhao H, Tanaka T, Halicka HD, Traganos F, Zarebski M, Dobrucki J, Darzynkiewicz Z. Cytometric assessment of DNA damage by exogenous and endogenous oxidants reports the aging-related processes. Cytometry A 2007; 71A:905-914.

    601. Hsieh T-C, Traganos F, Darzynkiewicz Z, Wu JM.The 2,6-disubstituted purine reversine induces growth arrest and polyploidy in human cancer cells. Int J Oncol 2007; 31:1293-1300.

    602. Ardelt B, Ardelt W, Pozarowski P, Kunicki J, Shogen K, Darzynkiewicz Z. Cytostatic and cytotoxic properties of Amphinase, a novel cytotoxic ribonuclease from Rana pipiens oocytes. Cell Cycle 2007; 6:3097-3102.

    2008

    603. Smart DJ, Halicka HD, Traganos F, Darzynkiewicz Z, Williams GM. Ciprofloxacin-induced G2 arrest and apoptosis in TK6 lymphoblastoid cells is not dependent on double-strand break formation. Cancer Biol & Ther 2008; 7:113-119.

    604. Halicka HD, Ita M, Tanaka T, Kurose A, Darzynkiewicz Z. The biscoclaurine alkaloid cepharanthine protects DNA in TK6 lymphoblastoid cells from constitutive oxidative damage. Pharmacol Rep 2008; 60:93-100.

    605. Darzynkiewicz Z, Galkowski D, Zhao H. Analysis of apoptosis by cytometry using TUNEL assay. Methods 2008; 44:250-254.

    606. Chen S, Gao J, Halicka HD, Traganos F, Darzynkiewicz Z. Down-regulation of androgen-receptor and PSA by phytochemicals. Int J Oncol 2008; 32:405-411.

    607. Wang X, Yang Y, Jiang N, Duan Q, Darzynkiewicz Z, Dai W. Sgo1 functions in centriole cohesion, which is partly regulated by Plk1. Dev Cell 2008; 14:331-341.

    608. Wlodkowic D, Skommer J, Darzynkiewicz Z. SYTO probes in cytometry of tumor cell death Cytometry A 2008; 73A:496-507.

    609. Zhao H, Tanaka T, Mitlitski V, Heeter J, Balazs EA, Darzynkiewicz Z. Protective effect of hyaluronate on oxidative DNA damage in WI-38 and A549 cells. Int J Oncol 2008; 32:1159-1169.

    610. Ardelt W, Shogen K, Darzynkiewicz Z. Onconase and amphinase, the anti-tumor ribonucleases from Rana pipiens oocytes. Curr Pharm Biotechnol. 2008; 9:215-225.

    611. Zhao H, Traganos F, Albino AP, Darzynkiewicz Z. Oxidative stress induces cell cycle-dependent Mre11 recruitment, ATM and Chk2 activation and histone H2AX phosphorylation Cell Cycle 2008; 7:1490-1495.

    613. Smart DJ, Halicka HD, Schmuck G, Traganos F, Darzynkiewicz Z, Williams GM. Assessment of DNA double-strand breaks and gH2AX induced by the topoisomerase II poisons etoposide and mitoxantrone. Mutat Res 2008; 641:43-47.

    614. Pietrzak M, Halicka HD Wieczorek Z, Wieczorek J, Darzynkiewicz Z. Attenuation of acridine mutagen ICR 191-DNA interactions and DNA damage by the mutagen interceptor chlorophyllin. Biophys J 2008; 135:69-75.

    615. Zhao H, Traganos F, Darzynkiewicz Z. Kinetics of histone H2AX phosphorylation and Chk2 activation in A549 cells treated with topotecan and mitoxantrone in relation to the cell cycle phase. Cytometry A 2008; 73A:480-489.

    616. Wlodkowic D, Skommer J, Hillier C, Darzynkiewicz Z. Multiparameter detection of apoptosis using red-excitable SYTO probes. Cytometry A 2008;73A:563-569, 2008.

    617. Ita M, Halicka HD, Tanaka T, Kurose A, Ardelt B, Shogen K, Darzynkiewicz Z. Remarkable enhancement of cytotoxicity of onconase and cepharanthine when used in combination on various tumor cell lines. Cancer Biol & Ther 2008; 7:1104-1108.

    618. Zhao H, Traganos F, Darzynkiewicz Z. Phosphorylation of p53 on Ser15 during cell cycle caused by Topo I and Topo II inhibitors in relation to ATM and Chk2 activation. Cell Cycle 2008; 7:3048-3055.

    619. Zhao H, Ardelt B, Ardelt W, Shogen K, Darzynkiewicz, Z. The cytotoxic ribonuclease onconase targets RNAi (siRNA). Cell Cycle 2008; 7:3258-3261.

    620. Darzynkiewicz Z. Pushing cancer cells to commit suicide: is 072RB, the new BH3 mimetic, up to the job? Cell cycle 2008; 20:3111 (Commentary)

    2009

    621. Tanaka T, Halicka D, Traganos F, Darzynkiewicz Z. Cytometric analysis of DNA damage: phosphorylation of histone H2AX as a marker of DNA double-strand breaks (DSBs). Meth Mol Biol 2009; 523:161-168.

    622. Halicka HD, Mitlitski V, Heeter J, Balazs EA, Darzynkiewicz Z. Attenuation of the oxidative burst induced DNA damage in human leukocytes by hyaluronate. Int J Mol Med 2009; 23:695-699.

    623. Wlodkowic D, Skommer J, Faley S, Preckel T, Darzynkiewicz Z, Cooper JM. Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry. Exp Cell Res 2009; 315:1706-1714.

    624. Halicka HD, Ozkaynak MF, Levendoglu-Tugal O, Sandoval C, Seiter K, Kajstura M, Traganos F, Jaybose S, Darzynkiewicz Z. DNA damage response as a biomarker in treatment of leukemias. Cell Cycle 2009; 8:1720-1724.

    625. Sato Y, Kurose A, Ogawa A, Ogasawara K, Traganos F, Darzynkiewicz Z, Sawai T. Diversity of DNA damage response of astrocytes and different glioblastoma cell lines with various p53 status to treatment with etoposide and temozolamide. Cancer Biol Ther 2009; 8:425-427..

    626. Zhao H, Traganos F, Dobrucki J, Wlodkowic D, Darzynkiewicz Z. Induction of DNA damage response by the supravital probes of nucleic acids. Cytometry A 2009; 75A:510-519.\

    627. Hao S, Zhao H, Darzynkiewicz Z, Battula S, Ferreri NR.Expression and function of NFAT5 in medullary thick ascending limb (mTAL) cells. Am J Physiol Renal Physiol 2009 296:F1494-14303.

    628. Wlodkowic D, Skommer J, Darzynkiewicz Z. Flow cytometrybased apoptosis detection. Meth Mol Biol 2009; 559:19-32.

    629. Halicka HD, Zhao H, Podhorecka M, Traganos F, Darzynkiewicz, Z. Cytometric detection of chromatin relaxation, an early reporter of DNA damage response. Cell Cycle 2009; 8: 2233-2237

    630. Albino AP, Jorgensen E, Rainey P, Gillman G, Clark TJ, Gietl, D, Zhao H, Traganos F, Darzynkiewicz Z. ?H2AX: A potential DNA damage response biomarker for assessing toxicological risk of tobacco products. Mutation Res 2009; 678:43-52.

    631. Darzynkiewicz Z. Forever young, fit and trim. Rapamycin to the rescue. Cell Cycle 2009; 8: 1820-1821.

    632. Darzynkiewicz Z, Halicka HD, Tanaka T.Cytometric assessment of DNA damage induced byDNA topoisomerase inhihibitors. Meth Molec Biol. 2009; 582: 145-153.

    633. Zhao H, Albino AP, Jorgensen E, Traganos F, Darzynkiewicz Z. DNA damage response induced by tobacco smoke in normal human bronchial epithelial and A549 pulmonary adenocarcinoma cells assessed by laser scanning cytometry. Cytometry A 2009; 75A: 840-847.

    634. Halicka HD, Darzynkiewicz Z, Teodori L. Attenuation of constitutive ATM activation and H2AX phosphorylation in human leukemic TK6 cells by their exposure to static magnetic field. Cell Cycle, 2009; 8:3236-3238.

    635. Wlodkowic D, Skommer J, McGuinness D, Hillier C, Darzynkiewicz Z. ER-Golgi network- a future target for anti-cancer therapy. Leuk Res 2009; 33:1440-1447

    636. Halicka HD, Smart DJ, Traganos F, Williams GM, Darzynkiewicz Z. Fluoroquinolones lower constitutive H2AX and ATM phosphorylation in TK6 lymphoblastoid cells via modulation of intracellular redox status. Pharmacol Rep 2009; 61:711-718.

    637. Darzynkiewicz Z. When senescence masquerades as DNA damage: is DNA replication stress the culprit? Cell Cycle 2009; 8: 3810-3811.

    638. Darzynkiewicz Z, Traganos F, Wlodkowic D. Impaired DNA damage response an Achilles heel sensitizing cancer to chemotherapy and radiotherapy. Eur J Pharmacol 2009; 625:143150

    639. Ardelt W, Ardelt B, Darzynkiewicz Z. Ribonucleases as potential modalities in anticancer therapy. Eur J Pharmacol 2009; 625:181-189.

    640. Wlodkowic D, Skommer J, McGuinness D, Faley S, Kolch W, Darzynkiewicz Z, Cooper, J.Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cells. Analyt Chem 2009; 81; 6952-6960

    641. Darzynkiewicz Z, Halicka HD, Zhao H. Analysis of cellular DNA by flow and laser scanning cytometry. In Polyploidization and Cancer. Ed. R.Y.C. Poon. Landes Bioscience. Madame Curie Database, July 2009

    2010

    642. Pozarowska D, Pozarowski P, Darzynkiewicz Z. Cytometric assessment of cytostatic and cytotoxic effects of topical glaucoma medications on human epithelial corneal line cells. Cytometry B. Clin Cytometry. 2010; 78B:130-137.

    643. Zhao, H., Traganos F, Darzynkiewicz Z. Kinetics of the UV-induced DNA damage response in relation to cell cycle phase. Correlation with DNA replication.Cytometry A, 2010; 77A: 285-293.

    644. Ikeda M, Kurose A, Takatori E, Sugiyama T, Traganos F, Darzynkiewicz Z, Sawai T. DNA damage detected with ?H2AX on endometrioid adenocarcinoma cell lines. Int J Oncol 2010; 36:1081-1088.

    645. Zhao H, Oczos J, Janowski P, Trembecka D, Dobrucki J., Darzynkiewicz Z, Wlodkowic D. Rationale for the real-time and dynamic cell death assays using propidum iodide. Cytometry A, 2010; 77A:399-405.

    646. Oka K, Tanaka T, Enoki T, Yoshimura K, Ohshima M, Kubo M, Murakami T, Gondou T, Minami Y, Takemoto Y, Harada E, Tsushimi T, Li T-S, Traganos F, Darzynkiewicz Z, Hamano K. DNA damage signaling is activated during cancer progression in human colorectal carcinoma. Cancer Biol Ther 2010; 9: 246-252.

    647. Darzynkiewicz Z. Critical aspects in analysis of cellular DNA content. Curr Protocols in Cytometry. 2010, April 10, Chapter 7; Unit 7.2

    648. Jorgensen ED, Zhao H, Traganos F, Albino AP, Darzynkiewicz Z. DNA damage response induced by exposure of A549 human lung adenocarcinoma cells to smoke from tobacco- and nicotine-free cigarettes. Cell Cycle, 2010, 9:2170-2176 (open access article).

    649. Skommer J, Darzynkiewicz Z, Wlodkowic D.Cell death goes LIVE: Technological advances in real-time tracking of cell death. Cell Cycle 2010; 9: 2330-2441. (open access article)

    650. Darzynkiewicz Z. Guarding genome integrity in stem cells. Commentary. Cell Cycle 2010; 9:2059-2960.

    651. Darzynkiewicz Z. Another Janus paradox of p53: induction of cell senescence versus quiescence. Commentary. Aging (Albany) 2010; June 20, 2(6) 329-330.

    652. Wlodkowic D, Skommer J, Darzynkiewicz Z. Cytometry in cell necrobiology revisited.Recent advances and new vistas. Cytometry A, 2010; 77A:591-606.

    653. Darzynkiewicz Z, Halicka HD, Zhao H. Analysis of cellular DNA by flow and laser scanning cytometry. Adv Exp Med Biol 2010; 676:137-147.

    654. Darzynkiewicz Z. Cytometry of the cell cycle: In search for perfect methodology for DNA content analysis in tissue specimens.Commentary. Cell Cycle 2010; 9: 3584-3585.

    655. Zhao H, Halicka HD, Jorgensen E, Traganos F, Darzynkiewicz Z. New biomarkers probing the depth of cell senescence assessed by laser scanning cytometry.Cytometry A, 2010, 77A, 999-1007.

    656. Wlodkowic D, Darzynkiewicz Z. Microfluidics: Emerging prospects for anti-cancer drug screening. World J Clin Oncol 2010;1:18-23.

    2011

    657. Darzynkiewicz Z, Zhao H. Detection of DNA strand breaks in apoptotic cells by flow- and image- cytometry. Meth Mol Biol 2011; 682: Chapter 8, pp. 91-110

    658. Darzynkiewicz Z, Pozarowski P, Lee B, Johnson GL. Fluorochrome-labeled inhibitors of caspases (FLICA): Convenient in vitro and in vivo markers of apoptotic cells for cytometric analysis. Meth Mol Biol 2011; 682: Chapter 9, pp.103-114.

    659. Darzynkiewicz Z, Smolewski P, Holden E, Luther E, Henriksen M, Franois M, Leifert W, Fenech M. Laser scanning cytometry for automation of the micronucleus assay. Mutagenesis 2011; 1:153-161

    660. Nowicki TS, Zhao H, Darzynkiewicz Z, Moscatello A, Shin E, Schantz S, Tiwari RK, Geliebter J. Down-regulation of uPAR inhibits migration, invasion, proliferation, and FAK/PI3K/Akt signaling in papillary thyroid carcinoma cells. Cell Cycle, 2011; 10:100-107.

    661. Hao S, Zhao H, Darzynkiewicz Z, Battula S, Ferreri NR. Differential regulation of NFAT5 by NKCC2 isoforms in medullary thick ascending limb (mTAL) cells. Am J Physiol Renal Physiol 2011; 300 (4):F966-7512. Epub 2011 Jan 12.

    662. Darzynkiewicz Z, Zhao H, Halicka HD, Li J. Cytometry of DNA replication and RNA synthesis: Historical perspective and recent advances based on click chemistry Cytometry A, 2011; 79A; 328-337

    663. Oktay K, Goswani S, Darzynkiewicz Z. Manipulating ovarian aging: A new frontier in fertility preservation.Commentary. Aging (Albany) 2011, 3(1): Epub Jan 2011

    664. Darzynkiewicz Z. Novel strategies of protecting non-cancer cells during chemotherapy. Are they ready for clinical testing? Oncotarget 2011; 2:107-108.

    665. Wlodkowic D, Skommer J, Darzynkiewicz Z. Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes. Meth Mol Biol 2011; 740:91-89

    666. Wlodkowic D, Faley S, Darzynkiewicz Z, Cooper JM. Real-time cytotoxicity tests. Meth Mol Biol. 2011; 731:285-291.

    667. Liu X, Chervona Y, Tang M-S, Darzynkiewicz Z, Dai W. Chromium induces chromosomal instability, which is partly due to deregulation of BubR1 and Emi1, two APC/C inhibitors.Cell Cycle, 2011;10(14)

    668. Darzynkiewicz Z, Halicka HD, Zhao H. Cell synchronization by inhibitors of DNA replication induces replication stress and DNA damage response: analysis by flow cytometry. Methods Mol Biol 2011;761: 85-96.

    669. Wlodkowic D, Darzynkiewicz, Z. Rise of the micro-machines: microfluidics and the future of cytometry. Methods Cell Biol., 2011;102:105-125.

    670. Darzynkiewicz, Z., Traganos F, Zhao H, Halicka HD, Skommer J, Wlodkowic D. Analysis of individual molecular events of DNA damage response by flow and image-assisted cytometry. Methods Cell Biol., 2011;103:115-148.

    671. Wlodkowic D, Tellford W, Skommer J, Darzynkiewicz Z. Apoptosis and beyond: Cytometry in studies of programmed cell death. Methods Cell Biol, 2011; 103:55-98.

    672. Zhao H, Li J, Traganos F, Halicka HD, Zarebski M, Dobrucki J, Darzynkiewicz Z. Cell fixation in zinc salt solution (ZBF) is compatible with DNA damage response detection by phospho-specific antibodies. Cytometry A, 2011; 79A:470-476.

    673. Wlodkowic D, Khoshmanesh K, Sharpe JC, Darzynkiewicz Z, Cooper JC. Apoptosis goes on a chip: advances in the microfluidic analysis of programmed cell death. Analyt Chem 2011; 83: 6439-6446.

    674. Soleimani R, Heytens E, Darzynkiewicz Z, Oktay K. Mechanisms of chemotherapy-induced human ovarian aging: double styrand DNA breaks and microvascular compromise. Aging (Albany) 2011; 3:782-793.

    675. Zhao H, Dobrucki J, Rybak P, Traganos F, Halicka H.D. Darzynkiewicz Z. Induction of DNA damage signaling by oxidative stress in relation to DNA replication as detected using the Click Chemistry Cytometry A 2011; 79A: 897-902.

    676. Darzynkiewicz Z. Redunancy in response to DNA damage: The key to protection of genome integrity. Cell Cycle 2011; 10:3425-26.

    677. Halicka HD, Zhao H, Li J, Traganos F, Zhang S, Lee M, Darzynkiewicz Z Genome protective effect of metformin as revealed by reduced level of constitutive DNA damage signaling. Aging (Albany) 2011;3: 1028-1038.

    2012

    678. Zhao H, Dobrucki J, Rybak P, Traganos F, Darzynkiewicz Z. Relationship of DNA damage signaling induced by DNA topoisomerase inhibitors camptothecin/topotecan, mitoxantrone or etoposide and DNA replication. Cytometry A 1012; 81A: 45-51.

    679. Pozarowski P, Holden E, Darzynkiewicz Z. Laser scanning cytometry: Principles and applications. An update. Meth. Molec. Biol., (in press)

    680. Hong Z, Darzynkiewicz Z. Biomarkers of cell senescence assessed by imaging cytometry. Meth Molec Biol (in press)

    681. Glickman M, Parola AH, Darzynkiewicz Z, Nathan I.TPCK activates autophagic cell death in MCF-7 cells is associated with indirect down-regulation of intracellular proteases. Autophagy, (submitted)


    Contact: Zbigniew Darzynkiewicz