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LIST OF PUBLICATIONS: FULL PAPERS, PEER REVIEWED (>475) 1963 1. Darzynkiewicz, Z.: The use of vinyl superchloride in histologic technique. Folia Morphologica, (Warsaw) 14:97-100, 1963. 2. Ostrowski, K., Barnard, E.A., Stocka, Z., and Darzynkiewicz, Z.: Autoradiographic methods in enzyme cytochemistry. I. Localization of acetylcholinesterase activity using a 3H labeled irreversible inhibitor. Exp. Cell Res., 31:89-99, 1963. 3. Darzynkiewicz, Z.: Dynamics of incorporation of 35S sulphate into limb anlagen of chick embryos. Folia Histochem. Cytochem. 1:269-283, 1963. 4. Ostrowski, K., Darzynkiewicz, Z., Sawicki, W., and Stocka, Z.: The possibilities of application of the new model of an interference microscope (MPI) in biologic research. Folia Histochem. Cytochem., 1:553-562, 1963. 5. Sawicki, W., and Darzynkiewicz, Z.: The application of polyvinyl chloride films in autoradiography. Folia Histochem. Cytochem., 1:283-288, 1963. 1964 6. Ostrowski, K., Darzynkiewicz, Z., Sawicki, W., and Stocka, Z.: Dry mass of epithelial cells from the vagina of mice in the course of oestrus cycle measured by interference microscopy. Acta Biochim. Polonica, 11:99-105, 1964. 7. Ostrowski, K., Barnard, E.A., Darzynkiewicz, Z., and Rymaszewska, D.: Autoradiographic methods in enzyme cytochemistry. III. Measurements of esterases in the cells of mouse kidney. Exp. Cell Res., 36:43-52, 1964. 1965 8. Darzynkiewicz, Z., Jurukova, Z., and Wieckowski, J.: Interferometric measurements of the alkaline phosphatase activity in epithelial cells from the vagina of mice in the course of keratinization process. Bull. Acad. Pol. Sci., 13:201-205, 1965. 9. Wieckowski, J., and Darzynkiewicz, Z.: Studies on the kinetics of alkaline phosphatase reaction in Ehrlich ascites tumor cells with the use of interference microscopy. Experientia, 21:387-388, 1965. 10. Darzynkiewicz, Z., and Wieckowski, J.: Use of interference microscopy for quantitative estimation of alkaline phosphatase activity in Ehrlich ascites tumor cells. Folia Histochem. Cytochem., 3:275-289, 1965. 11. Zaleski, M., Rymaszewska, T., Darzynkiewicz, Z., and Wlodarski, K.: Autoradiographic studies of cells from lymph nodes draining the tissue graft area. Bull. Acad. Pol. Sci., 13:43-47, 1965. 12. Kawiak, J., Moskalewski, S., and Darzynkiewicz, Z.: Isolation of chondrocytes from nasal cartilage of calf. Exp. Cell Res., 39:59-68, 1965. 13. Darzynkiewicz, Z., Krassowski, T., and Skopinska, E.: Stimulating effect of phytohemagglutinin upon "rapidly labelled" ribonucleic acid synthesis in human lymphocytes. Bull. Acad. Pol. Sci., 13:433-437, 1965. 14. Darzynkiewicz, Z., Krassowski, T., and Skopinska, E.: Effect of phytohemagglutinin on synthesis of "rapidly labeled" ribonucleic acid in human lymphocytes. Nature, 207:1402, 1965. 1966 15. Darzynkiewicz, Z., Rogers, A.W., and Barnard, E.A.: Measurements of esterases in rat megakaryocytes by the labeled inhibitor method. J. Histochem. Cytochem., 14:807, 1966. 16. Jablonska, S., Langner, A., Stachow, A., and Darzynkiewicz, Z.: L'importance generale d'acantholyse. In: "Pemphigus, occupational dermatoses due to chemical sensitization." Budapest, pp. 362-365, 1966. 17. Darzynkiewicz, Z., Rogers, A.W., Barnard, E.A., Wang, D.H., and Werkheiser, W.C.: Autoradiography with tritiated methotrexate and the cellular distribution of folate reductase. Science, 151:1528-1530, 1966. 18. Darzynkiewicz, Z., Rogers, A.W., and Barnard, E.A.: Quantitative analysis of the esterases in rat megakaryocytes by radioisotope cytochemical technique. J. Histochem. Cytochem., 14:379-384, 1966. 19. Darzynkiewicz, Z., Rogers, A.W., and Barnard, E.A.: The number of acetyl-cholinesterase molecules in the rat megakaryocyte. J. Histochem. Cytochem., 14:915-922, 1966. 20. Rogers, A.W., Darzynkiewicz, Z., Barnard, E.A., and Salpeter, M.M.: Number and location of acetylcholinesterase molecules at motor endplates of the mouse. Nature, 210:1003 1006, 1966. 1967 21. Darzynkiewicz, Z., and Barnard, E.A.: Specific proteases of the rat mast cell. Nature, 213:1198-1202, 1967. 22. Budd, G.C., Darzynkiewicz, Z., and Barnard, E.A.: Intracellular localization of specific proteases in rat mast cells. Nature, 213:1202-1203, 1967. 23. Darzynkiewicz, Z., Dokov, V., and Pienkowski, M.: Dry mass of lymphocytes during transformation after stimulation by phytohemagglutinin. Nature, 214:1265-1266, 1967. 24. Darzynkiewicz, Z., Langner, A., and Pienkowski, M.: Effect of penicillin on RNA synthesis in lymphocytes of penicillin-sensitized individuals. Experientia, 23:840, 1967 25. Darzynkiewicz, Z., and Komender, J.: Autoradiographic detection of sodium-24 ions in single cells. J. Histochem. Cytochem.. 15:605-607, 1967. 1968 26. Langner, A., Jablonska, S., and Darzynkiewicz, Z.: Autoradiographic studies on DNA synthesis by epidermal cells in epidermodysplasia verruciformis. Acta Dermatol. Venerol., (Stockholm) 48:501-510, 1968. 1969 27. Darzynkiewicz, Z., and Pienkowski, M.: Effect of prednisolone on RNA synthesis in human lymphocytes stimulated by phytohemagglutinin. Exp. Cell Res., 54:289-292, 1969. 28. Darzynkiewicz, Z., and Pienkowski, M.: Autoradiographic studies on the stability of ribonucleic acid in phytohemagglutinin-stimulated lymphocytes. Exp. Cell Res., 55:118-120, 1969. 29. Rogers, A.W., Darzynkiewicz, Z., Salpeter, M.M., Ostrowski, K., and Barnard, E.A.: Quantitative studies on enzymes in structures of striated muscles by labeled inhibitor methods. J. Cell Biol., 41:665-689, 1969. 30. Darzynkiewicz, Z., Bolund, L., and Ringertz, N.R.: Actinomycin D binding of normal and phytohemagglutinin stimulated lymphocytes. Exp. Cell Res., 55:120-126, 1969. 31. Bolund, L., Darzynkiewicz, Z., and Ringertz, N.R.: Growth of hen erythrocytes nuclei undergoing reactivation in heterokaryons. Exp. Cell Res., 56:406-410, 1969. 32. Ringertz, N.R., Darzynkiewicz, Z., and Bolund, L.: Actinomycin D binding properties of stimulated human lymphocytes. Exp. Cell Res., 56:411-417, 1969. 33. Darzynkiewicz, Z., Bolund, L., and Ringertz, N.R.: Nucleo protein changes and initiation of RNA synthesis in PHA stimulated lymphocytes. Exp. Cell Res., 56:418-424, 1969. 34. Darzynkiewicz, Z., Gledhill, B.L., and Ringertz, N.R.: Changes in deoxyribo-nucleoprotein during spermiogenesis in the bull. 3H-Actinomycin D binding. Exp. Cell Res., 58:435-438, 1969. 35. Langner, A., Jablonska, S. and Darzynkiewicz, Z.: Studies on the origin of the mucin in mucinosis follicularis. Acta Derm. Venerol., (Stockholm) 49: 76-81, 1969. 1970 36. Ringertz, N.R., Gledhill, B.L., and Darzynkiewicz, Z.: Changes in deoxyri-bonucleoprotein during spermiogenesis in the bull. II. Sensitivity of DNA to heat denaturation. Exp. Cell Res., 62:204-218, 1970. 37. Bolund, L., Darzynkiewicz, Z., and Ringertz, N.R.: Cell concentration and the staining properties of nuclear deoxyribonucleoprotein. Exp. Cell Res., 62:76-83, 1970. 38. Ringertz, N.R., Bolund, L., and Darzynkiewicz, Z.: Acridine orange binding of intracellular nucleic acids in fixed cells in relation to cell growth. Exp. Cell Res., 63:233-238, 1970. 1971 39. Darzynkiewicz, Z., and Jacobson, B.: HEPES-buffered media in lymphocyte cultures. Proc. Soc. Exp. Biol. Med., 136:387-393, 1971. 40. Gledhill, B.L., Darzynkiewicz, Z., and Ringertz, N.R.: Changes in deoxyribonucleoprotein during spermiogenesis in the bull. Increased 3H-Actinomycin D binding to nuclear chromatin of morphologically abnormal spermatozoa. J. Reprod. Fertil., 26:25-38, 1971. 41. Darzynkiewicz, Z., and Balazs, E.A.: Effect of connective tissue intercellular matrix on lymphocyte stimulation. I. Suppression of lymphocyte stimulation by hyaluronic acid. Exp. Cell Res., 66:113-123, 1971. 42. Darzynkiewicz, Z., and Andersson, J.: Effect of prednisolone on thymus lymphocytes. I. Autoradiographic studies on 3H-Actinomycin D binding and 3H-uridine incorporation. Exp. Cell Res., 67:39-48, 1971. 43. Darzynkiewicz, Z., and Jacobson, B.: Effect of prednisolone on thymus lymphocytes. II. Studies on 3H-Actinomycin D binding and RNA polymerase activity of isolated nuclei. Exp. Cell Res., 67:49-60, 1971. 44. Darzynkiewicz, Z., and Pienkowski, M.: Studies on lymphocyte stimulation. Acta Physiol. Pol., (Warsaw) 22:791-798, 1971 (in Polish). 45. Darzynkiewicz, Z.: Radiation-induced DNA synthesis in nuclei of hen erythrocytes reactivated in heterokaryons. Exp. Cell Res., 69:477-481, 1971. 46. Darzynkiewicz, Z.: Radiation-induced DNA synthesis in normal and stimulated human lymphocytes. Exp. Cell Res., 69:356-360, 1971. 1972 47. Ritzen, M., Carlsson, S.A., and Darzynkiewicz, Z.: Cytochemical evidence of nucleoprotein changes in rat adrenal cortex following hypophysectomy or dexamethasone suppression. Exp. Cell Res., 70:46-50, 1972. 48. Penn, A., Gledhill, B., and Darzynkiewicz, Z.: Modification of the gelatin substrate procedure for demonstration of acrosomal proteolytic activity. J. Histochem. Cytochem., 20:499-506, 1972. 49. Darzynkiewicz, Z., and Chelmicka-Szorc, E.: Unscheduled DNA synthesis in hen erythrocyte nuclei reactivated in heterokaryons. Exp. Cell Res., 74:131-139, 1972. 50. Darzynkiewicz, Z., Chelmicka-Szorc, E., and Arnason, B.G.W.: U.V.-induced DNA synthesis in nuclei of Xeroderma Pigmento sum cells in heterokaryons. Exp. Cell Res., 74:602-608, 1972. 51. Anderson, J., and Darzynkiewicz, Z.: Nucleoprotein changes and uridine incorporation in rat thymus lymphocytes. I. Cellular characterization of age-dependent thymus involution. Exp. Cell Res., 75:410-415, 1972. 52. Anderson, J., and Darzynkiewicz, Z.: Nucleoprotein changes and uridine incorporation in rat thymus lymphocytes. II. Effect of treatment with anti-thymocyte serum in vitro. Exp. Cell Res., 75:417-423, 1972. 53. Darzynkiewicz, Z., and Balazs, E.A.: Effect of Connective Tissue Intercellular Matrix on Lymphocyte Stimulation. In: Experimental Control of Mitosis, Vol. II. McCormick et al., (Eds.), MSS Information Corp., New York, pp. 160-170, 1972. 1973 54. Chakrabarti, B., and Darzynkiewicz, Z.: Cortisol-induced changes in U.V. absorption spectra of isolated nuclei from rat liver and thymus. Biochem. Biophys. Res. Commun., 50:164-171, 1973. 55. Gledhill, B.L., and Darzynkiewicz, Z.: Unscheduled DNA synthesis during mammalian spermatogenesis in response to U.V. irradiation. J. Exp. Zool., 183:375-382, 1973. 56. Darzynkiewicz, Z.: Detection of DNA polymerase activity in fixed cells. Exp. Cell Res., 80:483-486, 1973. 57. Darzynkiewicz, Z., and Gledhill, B.L.: Uptake of tritium labeled adenosine triphosphate by the cytoplasm of fixed interstitial cells from rat testes. J. Histochem. Cytochem., 21:1035-1040, 1973. 58. Balazs, E.A., and Darzynkiewicz, Z.: The effect of hyaluronic acid on fibroblasts, mononuclear phagocytes and lymphocytes. In: Biology of the Fibroblast, Papers of the Symposium held in Turku, Finland, 1972; Kulonen, E., and Pikkarainen, J., (Eds.), Academic Press, London, pp. 237-252, 1973. 1974 59. Darzynkiewicz, Z., and Arnason, B.G.W.: Suppression of RNA synthesis in lymphocytes by inhibitors of proteolytic enzymes. Exp. Cell Res., 85:95-104, 1974. 60. Darzynkiewicz, Z., Chelmicka-Szorc, E., and Arnason, B.G.W.: Suppressive effect of protease inhibitors on heterokaryons containing chick erythrocyte nuclei. Exp. Cell Res., 87:333-345, 1974. 61. Darzynkiewicz, Z., Chelmicka-Szorc, E., and Arnason, B.G.W.: Chick erythrocyte nucleus reactivation in heterokaryons: Suppression by inhibitors of proteolytic enzymes. Proc. Natl. Acad. Sci. USA, 71:644-647, 1974. 62. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Thermally-induced changes in chromatin of isolated nuclei and of intact cells as revealed by acridine orange staining. Biochem. Biophys. Res. Commun., 59:392-399, 1974. 1975 63. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry. Exp. Cell Res., 90:411-428, 1975 (Reprinted in Yearbook of Cancer, 1975). 64. Braunstein, J.D., Melamed, M.R., Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Good, R.A.: Quantitation of transformed lymphocytes by cytofluorometry. I. Phytohemagglutinin response. Clin. Immunol. Immunopathol., 4:209-215, 1975. 65. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Acridine orange staining and automated cytofluorometry in studies of conformation changes in nuclear chromatin. In: Pulse Cytophotometry, Haanen, C.A.M. et al., (Eds.), European Press-Medicon, Ghent, Belgium, pp. 88-102, 1975. 66. Traganos, F., Darzynkiewicz, Z., Sharpless, T., and Melamed, M.R.: Denaturation of deoxyribonucleic acid in situ: Effect of formaldehyde. J. Histochem. Cytochem., 23:431-438, 1975. 67. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Conformation of RNA in situ as studied by acridine orange staining and automated cytofluorometry. Exp. Cell Res., 95:143-153, 1975. 68. Sharpless, T., Traganos, F., Darzynkiewicz, Z., and Melamed, M.R.: Flow cytofluoro-metry: Discrimination between single cells and cell aggregates by direct size measure-ments. Acta Cytol., 19:577-581, 1975. 1976 69. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: DNA denaturation in situ. Effect of divalent cations and alcohols. J. Cell Biol., 68:1-10, 1976. 70. Traganos, F., Darzynkiewicz, Z., Sharpless, T., and Melamed, M.R.: Cytofluorometric studies on conformation of nucleic acids in situ. I. Restriction of acridine orange binding by chromatin proteins. J. Histochem. Cytochem., 24:40-48, 1976. 71. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Cytofluorometric studies on conformation of nucleic acids in situ. II. Denaturation of DNA. J. Histochem. Cytochem., 24:49-58, 1976. 72. Darzynkiewicz, Z., Traganos, F., Sharpless, T., Friend, C., and Melamed, M.R.: Nuclear chromatin changes during erythroid differentiation of Friend-virus induced leukemic cells. Exp. Cell Res., 99:301-309, 1976. 73. Melamed, M.R., Traganos, F., Sharpless, T., and Darzynkiewicz, Z.: Urinary cytology automation: Preliminary studies with acridine orange staining and flow-through cytofluo-rometry. Invest. Urol., 13:331-338, 1976. 74. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Effect of 0.25 M sodium chloride treatment on DNA denaturation in situ in thymus lymphocytes. Exp. Cell Res., 100:393-396, 1976. 75. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Lymphocyte stimulation: A rapid multiparameter analysis. Proc. Natl. Acad. Sci. USA, 73:2881-2884, 1976. 76. Darzynkiewicz, Z.: Potential of fluorescent molecular probes as markers for instrumental evaluation of cells in the female reproduction tract. In: The Automation of Uterine Cancer Cytology, Wied, G.I., Bahr, G.F., and Bartels, P.H. (Eds.), Tutorials of Cytology, Chicago, Ill., pp. 364-369, 1976. 1977 77. Traganos, F., Darzynkiewicz, Z., Sharpless, T., and Melamed, M.R.: Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J. Histochem. Cytochem., 25:46-56, 1977. 78. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Different sensitivity of DNA in situ in interphase and metaphase chromatin to heat denaturation. J. Cell Biol., 73:128-138, 1977. 79. Melamed, M.R., Darzynkiewicz, Z., Traganos, F., and Sharpless, T.: Nucleic acid content and nuclear chromatin structure of human bladder cell lines as studied by flow cytofluorometry. Cancer Res., 37:1227-1232, 1977. 80. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Recognition of cells in mitosis by flow cytofluorometry. J. Histochem. Cytochem., 25:875-880, 1977. 81. Traganos, F., Gorski, A.J., Darzynkiewicz, Z., Sharpless, T., and Melamed, M.R.: Rapid multiparameter analysis of cell stimulation in mixed lymphocyte culture (MLC) reaction. J. Histochem. Cytochem., 25:881-887, 1977. 82. Melamed, M.R., Darzynkiewicz, Z., Sharpless, T., and Traganos, F.: Cytology automation by flow cytometry. Cancer Res. 37:2806-2812, 1977. 83. Traganos, F., Darzynkiewicz, Z., Sharpless T., and Melamed, M.R.: Nucleic acid content of cell cycle distribution of five human bladder cell lines analyzed by flow cytofluorometry. Int. J. Cancer, 20:30-36, 1977. 84. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Cell cycle related changes in nuclear chromatin of stimulated lymphocytes as measured by flow cytometry. Cancer Res.. 37:4635-4640, 1977. 85. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Interphase and metaphase chromatin: Different stainability of DNA with acridine orange. Exp. Cell Res., 110:201-214, 1977. 1978 86. Kurland, J., Traganos, F., Darzynkiewicz, Z., and Moore, M.: Macrophage mediated cytostasis of neoplastic hematopoietic cells. Cytofluorometric analysis of cell block. Cell Immunol., 36:318-330, 1978. 87. Darzynkiewicz, Z., Andreeff, M., Traganos, F., Sharpless, T., and Melamed, M.R.: Discrimination of cycling and noncycling lymphocytes by BrdUrd-suppressed acridine orange fluorescence in a flow cytometric system. Exp. Cell Res., 115:31-35, 1978. 88. Andreeff, M., Beck, J.D., Darzynkiewicz, Z., Traganos, F., Gupta, S., Melamed, M.R., and Good, R.A.: RNA content in human lymphocyte subpopulations. Proc. Natl. Acad. Sci. USA, 75: 1938-1942, 1978. 89. Ashihara, T., Traganos, F., Baserga, T., and Darzynkiewicz, Z.: A comparison of cell cycle related changes in post mitotic and quiescent AF8 cells as measured by flow cytometry after acridine orange staining. Cancer Res., 38:2514-2518, 1978. 90. Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.R.: Differential Stainability of M vs G2 and G0 vs G1 cells. In: Pulse Cytophotometry III International Symposium, Lutz, D., ed., European Press, Ghent, Belgium, pp. 262-273, 1978. 91. Chelmicka-Schorr, E., Arnason, B.G.W., Astrom, K.E., and Darzynkiewicz, Z.: Treatment of mouse muscular dystrophy with the protease inhibitor pepstatin. J. Neuropathol. Exp. Neurol., 37:263-268, 1978. 1979 92. Traganos, F., Darzynkiewicz, Z., Sharpless, T., and Melamed, M.R.: Erythroid differentiation of Friend leukemia cells as studied by acridine orange staining and flow cytometry. J. Histochem. Cytochem., 27:382-389, 1979. 93. Darzynkiewicz, Z., Traganos, F., Andreeff, M., Sharpless, T., and Melamed, M.R.: Different sensitivity of chromatin to acid denaturation in quiescent and cycling cells as revealed by flow cytometry. J. Histochem. Cytochem., 27:478-485, 1979. 94. Collste, L., Darzynkiewicz, Z., Traganos, F., Sharpless, T., Whitmore, W.F., and Melamed, M.R.: Identification of polymorphonuclear leukocytes in cytological samples by flow cytometry. J. Histochem. Cytochem., 27:390-394, 1979. 95. Darzynkiewicz, Z., Evenson, D., Staiano-Coico, L., Sharpless, T., and Melamed, M.R.: Relationship between RNA content and progression of lymphocytes through the S phase of the cell cycle. Proc. Natl. Acad. Sci. USA, 76:358-362, 1979. 96. Collste, L.G., Darzynkiewicz, Z., Traganos, F., Devonec, M., Claps, M.L.K., Whitmore, W.F., Jr., and Melamed, M.R.: Cell cycle distribution of urothelial tumour cells as measured by flow cytometry. Br. J. Cancer, 40:872-877, 1979. 97. Collste, L.G., Darzynkiewicz, Z., Traganos, F., Sharpless, T., Whitmore, W.F., Jr., and Melamed, M.R.: Regional lymph node reactivity in explanted bladder cancer of mice as measured by flow cytometry. Cancer Res., 39:2120-2124, 1979. 98. Evenson, D.P., Darzynkiewicz, Z., Staiano-Coico, L., Traganos, F., and Melamed, M.R.: Effects of 9,10 Anthracendione, 1,4-bis 2-(hydroxyethyl)amino 2-ethyl amino-diacetate on cell survival and cell cycle progression in cultured mammalian cells. Cancer Res., 39:2574-2581, 1979. 99. Darzynkiewicz, Z., Evenson, D.P., Staiano-Coico, L., Sharpless, T., and Melamed, M.R.: Correlation between cell cycle duration and RNA content. J. Cell Physiol., 100:425-438, 1979. 100. Drapkin, R.L., Andreeff, M., Koziner, B., Strife, A., Wisniewski, D., Darzynkiewicz, Z., Melamed, M.R., and Clarkson, B.: Subpopulation of human peripheral blood cells: Analysis of granulocytic progenitor cells by flow cytometry and immunologic surface markers. Am. J. Hematol., 7:163-172, 1979. 101. Eisinger, M., Lee, J.S., Hefton, J.M., Darzynkiewicz, Z., Chiao, J.W., and deHarven, E.: Human epidermal cell cultures: Growth and differentiation in the absence of dermal components or medium supplements. Proc. Natl. Acad. Sci. USA, 76:5340, 1979. 102. Collste, L.G., Kostyrka-Claps, M.L., Darzynkiewicz, Z., Devonec, M. Traganos, F., Whitmore, W.F., and Melamed, M.R.: Lymph node reactivity to experimental bladder tumor in preimmunized animals as measured by two parameter flow cytometry. Invest. Urol., 17:191-194, 1979. 103. Andreeff, M. Beck, J.D., Darzynkiewicz, Z., Arlin, Z., Melamed, M.R., and Clarkson, B.: DNA, RNA and chromatin structure assayed in situ by flow cytometry: A means for the classification of human leukemias. Verh. Dtsch. Krebs. Ges. 2. Gustave Fischer Verlag, Stuttgart, New York, p. 348, 1979. 104. Andreeff, M., Beck, J.D., Darzynkiewicz, Z., Traganos, F., Tan, C., Clarkson, B., and Melamed, M.R.: Blastic chronic myeloid leukemia: Cell proliferation in vivo, in tissue culture, and after desacetyl amide (DVA). Verh. Dtsch. Krebs. Ges. 2. Gustave Fischer Verlag, Stuttgart, New York, p. 351, 1979. 1980 105. Mora, M., Darzynkiewicz, Z., and Baserga, R.: DNA synthesis and cell division in a mammalian cell mutant temperature sensitive for the processing of ribosomal RNA. Exp. Cell Res., 125:241-249, 1980. 106. Andreeff, M., Darzynkiewicz, Z., Sharpless, T.K., Clarkson, B.D., and Melamed, M.R.: Discrimination of human leukemia subtypes by flow cytometric analysis of cellular DNA and RNA. Blood, 55:282-293, 1980. 107. Evenson, D.P., Traganos, F., Darzynkiewicz, Z., Staiano-Coico, L., and Melamed, M.R.: Effects of 9,10-anthracenedione, 1,4-bis 2-(hydroxy-ethyl)amino ethyl amino-diacetate on cell morphology and nucleic acids of Friend leukemia cells. J. Natl. Cancer Inst., 64:857-866, 1980. 108. Traganos, F., Evenson, D.P., Staiano-Coico, L., Darzynkiewicz, Z., and Melamed, M.R.: The action of dihydroxyanthraquinone on cell cycle progression and survival of a variety of cultured mammalian cells. Cancer Res., 40:671-681, 1980. 109. Collste, L.G., Darzynkiewicz, Z., Traganos, F., Sharpless, T., Sogani, P., Grabstald, H., Whitmore, W.F., Jr., and Melamed, M.R.: Flow cytometry in cancer detection and evaluation using acridine orange metachromatic nucleic acid staining of irrigation cytology specimens. J. Urol., 123:478-485, 1980. 110. Collste, L.G., Devonec, M., Darzynkiewicz, Z., Traganos, F., Sharpless, T., Whitmore, W.F., Jr., and Melamed, M.R.: Bladder cancer diagnosis by flow cytometry. Correlation between cell samples from biopsy and bladder irrigation fluid. Cancer, 45:2389-2394, 1980. 111. Evenson, D.P., Darzynkiewicz, Z., and Melamed, M.R.: Comparison of human and mouse sperm chromatin by flow cytometry. Chromosoma, 78:225-238, 1980. 112. Pochron, S., Rossini, M., Darzynkiewicz, Z., Traganos, F., and Baserga, R.: Failure of accumulation of cellular RNA in hamster cells stimulated to synthesize DNA by infection with Adenovirus 2. J. Biol. Chem., 255:4411-4414, 1980. 113. Traganos, F., Staiano-Coico, L., Darzynkiewicz, Z., and Melamed, M.R.: Effects of ellipticine on cell cycle progression in cultured mammalian cells. Cancer Res., 40:2390-2399, 1980. 114. Petralia, S., Soprano, K.J., Pochron, R., Darzynkiewicz, Z., Traganos, F., and Baserga, R.: Increase in cellular RNA in cells infected with DNA oncogenic viruses. Cancer Res., 40:3177-3180, 1980. 115. Darzynkiewicz, Z., Traganos, F., and Melamed, M.R.: New cell cycle compartments identified by multiparameter flow cytometry. Cytometry, 1:98-108, 1980. 116. Evenson, D.P., Darzynkiewicz, Z., and Melamed, M.R.: Relation of mammalian sperm chromatin heterogeneity to fertility. Science, 210:1131-1133, 1980. 117. Traganos, F., Darzynkiewicz, Z., Staiano-Coico, L., Evenson, D., and Melamed, M.R.: Rapid, automatic analysis of the cell cycle point of action of cytostatic drugs. Flow Cytometry IV, Proceedings Fourth Internatl. Symp. Flow Cytometry, Voss, Norway, June 4-8, Universitetforlaget, Bergen, pp. 511-515, 1980. 118. Traganos, F., Darzynkiewicz, Z., and Melamed, M.R.: RNA content of the cell cycle. Flow Cytometry IV, Proceedings IVth Internatl. Symp. Flow Cytometry, Voss, Norway, June 4 8, Universitetforlaget, Bergen, pp. 331-335, 1980. 119. Darzynkiewicz, Z., Evenson, D., Staiano-Coico, L., Sharpless, T., and Melamed, M.R.: Duration of the G1 and S phase of individual cells in populations is correlated with their RNA content. Flow Cytometry IV, Proceedings IVth Internatl. Symp. Flow Cytometry, Voss, Norway, June 4-8, Universitetforlaget, Bergen, pp. 336-340, 1980. 120. Darzynkiewicz, Z., Andreeff, M., Traganos, F., and Melamed, M.R.: Proliferation and differentiation of normal and leukemic lymphocytes as analyzed by flow cytometry. Flow Cytometry IV, Proceedings IVth Internatl. Symp. Flow Cytometry, Voss, Norway, June 4-8, Universitetforlaget, Bergen, pp. 392-397, 1980. 121. Evenson, D.P., Darzynkiewicz, Z., and Melamed, M.R.: Flow cytometric analysis of sperm chromatin structure. Flow Cytometry IV, Proceedings IVth Internatl. Symp. Flow Cytometry, Voss, Norway, June 4-8, Universitetforlaget, Bergen, pp. 191-195, 1980. 122. Darzynkiewicz, Z., Sharpless, T., Staiano-Coico, L., and Melamed, M.R.: Subcompartments of the G1 phase of cell cycle detected by flow cytometry. Proc. Natl. Acad. Sci. USA, 77:6696-6700, 1980. 123. Traganos, F., Evenson, D.P., Staiano-Coico, L., Darzynkiewicz, Z., and Melamed, M.R.: Ellipticine induced changes in cell growth and nuclear morphology. J. Natl. Cancer Inst., 65:1329-1336, 1980. 124. Traganos, F., Staiano-Coico, L., Darzynkiewicz, Z., and Melamed, M.R.: Effects of prospidine on cell survival and growth of mammalian cells in culture. J. Natl. Cancer Inst., 65:993-999, 1980. 1981 125. Kapuscinski, J., Darzynkiewicz, Z., Traganos, F., and Melamed, M.R.: Interactions of a new antitumor agent 1,4-dihydroxy-5-8-bis 2-(hydroxyethyl)amino)ethyl)amino)--9,10-anthracenedione with nucleic acids. Biochem. Pharmacol., 30:231-240, 1981. 126. Darzynkiewicz, Z., Traganos, F., Xue, S., Staiano-Coico, L., and Melamed, M.R.: Rapid analysis of drug effects on the cell cycle. Cytometry, 1:279-286, 1981. 127. Traganos, F., Staiano-Coico, L., Darzynkiewicz, Z., and Melamed, M.R.: Effects of dihydro-5-azacytidine on cell survival and cell cycle progression of cultured mammalian cells. Cancer Res., 41:780-789, 1981. 128. Darzynkiewicz, Z., Traganos, F., and Melamed, M.R.: Detergent treatment for an alternative to cell fixation for flow cytometry. J. Histochem. Cytochem., 29:329-330, 1981. 129. Darzynkiewicz, Z., Staiano-Coico, L., and Melamed, M.R.: Increased mitochondrial uptake of rhodamine 123 during lymphocyte stimulation. Proc. Natl. Acad. Sci. USA, 78:2383-2387, 1981. 130. Olszewski, W., Darzynkiewicz, Z., Rosen, P., Schwartz, M.K., and Melamed, M.R.: Flow cytometry of breast carcinoma. I. Relation of DNA ploidy level to histology and estrogen receptors. Cancer, 48:980-984, 1981. 131. Olszewski, W., Darzynkiewicz, Z., Rosen, P., Schwartz, M.K., and Melamed, M.R.: Flow cytometry of breast carcinoma. II. Relation of tumor cell cycle distribution to histology and estrogen receptor. Cancer, 48:985-988, 1981. 132. Traganos, F., Staiano-Coico, L., Darzynkiewicz, Z., and Melamed, M.R.: Effects of aclacinomycin on cell survival and cell cycle progression of cultured mammalian cells. Cancer Res., 41:2728-2737, 1981. 133. Devonec, M., Darzynkiewicz, Z., Whitmore, W.F., and Melamed, M.R.: Flow cytometry for follow-up examinations of conservatively treated low stage bladder tumors. J. Urol., 126:166-170, 1981. 134. Darzynkiewicz, Z., Traganos, F., Xue, S., and Melamed, M.R.: Effect of n-butyrate on cell cycle progression and in situ chromatin structure of L1210 cells. Exp. Cell Res., 136:279-293, 1981. 135. Traganos, F., Darzynkiewicz, Z., and Melamed, M.R.: Effects of the L-isomer (+)-1,2-bis (3,5-dioxopiperazine-1-yl) propane on cell survival and cell cycle progression of cultured mammalian cells. Cancer Res., 41:4566-4576, 1981. 136. Preisler, H., and Darzynkiewicz, Z.: Flow cytometric analysis of proliferating and quiescent human leukemia cells. J. Med., 12:415-425, 1981. 137. Darzynkiewicz, Z., and Andreeff, M.: Multiparameter flow cytometry. Part I. Application in analysis of the cell cycle. Clinical Bulletin MSKCC, 11:47-87, 1981. 138. Andreeff, M. and Darzynkiewicz, Z.: Multiparameter flow cytometry. Part II. Application in clinical hematology. Clinical Bulletin MSKCC, 11:120-130, 1981. 1982 139. Devonec, M., Darzynkiewicz, Z., Kostyrka-Claps, M.L., Collste, L., Whitmore, W.F., and Melamed, M.R.: Flow cytometry of low stage bladder tumors: Correlation with cytologic and cystoscopic diagnosis. Cancer, 49:109-118, 1982. 140. Kapuscinski, J., Darzynkiewicz, Z., and Melamed, M.R.: Luminescence of the solid complexes of acridine orange with RNA. Cytometry, 2:201-211, 1982. 141. Traganos, F., Darzynkiewicz, Z., and Melamed, M.R.: The ratio of RNA to total nucleic acid content as a quantitative measure of unbalanced cell growth. Cytometry, 2:212-218, 1982. 142. Darzynkiewicz, Z., Traganos, F., Staiano-Coico, L., Kapuscinski, J.and Melamed, M.R.: Interactions of rhodamine 123 with living cells studied by flow cytometry. Cancer Res., 42:799-806, 1982. 143. Klein, F.A., Melamed, M.R., Whitmore, W.F., Herr, H.W., Sogani, P.C., and Darzynkiewicz, Z.: Characterization of bladder papilloma by two-parameter DNA-RNA flow cytometry. Cancer Res., 42:1094-1097, 1982. 144. Evenson, D.P., Darzynkiewicz, Z., and Melamed, M.R.: Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility. J. Histochem. Cytochem., 30:279-280, 1982. 145. Olszewski, W., Darzynkiewicz, Z., Claps, M.L., and Melamed, M.R.: Flow cytometry of lung carcinoma: A comparison of DNA stemline and cell cycle distribution with histology. Anal. Quant. Cytol., 4:90-94, 1982. 146. Staiano-Coico, L., Darzynkiewicz, Z., Melamed, M.R., and Weksler, M.: Changes in DNA content of human blood mononuclear cells with senescence. Cytometry, 3:79-83, 1982. 147. Darzynkiewicz, Z., Crissman, H., Traganos, F., and Stainkamp, J.: Cell heterogeneity during the cell cycle. J. Cell Physiol., 112:465-474, 1982. 148. Olszewski, W., Darzynkiewicz, Z., Rosen, P.P., Claps, M.L., and Melamed, M.R.: Flow cytometry of breast carcinoma. III. Possible altered kinetics in axillary lymph node metastases. Anal. Quant. Cytol., 4:275-278, 1982. 1983 149. Andreeff, M., and Darzynkiewicz, Z.: Chromatin structure in leukemia: Classification of subtypes and cell kinetics during induction. Cell Tissue Kinet., 15: 679-681, 1983. 150. Potmesil, M., Levin, M., Traganos, F., Darzynkiewicz, Z., Goldfeder, A., and Silber, R.: In vivo effects of adriamycin or N-trifluoroacetyladriamycin-14-valerate on mouse lymphoma. Eur. J. Cancer Clin. Oncol., 18:109-122, 1983. 151. Piwnicka, M., Darzynkiewicz, Z., and Melamed, M.R.: RNA and DNA content of isolated nuclei measured by multiparameter flow cytometry. Cytometry, 3:269-275, 1983. 152. Darzynkiewicz, Z., Traganos, F., and Melamed, M.R.: Distinction between 5-bromodeoxyuridine labelled and unlabelled mitotic cells by flow cytometry. Cytometry, 3:345-348, 1983. 153. Darzynkiewicz, Z.: New cell cycle compartments distinguished by multiparameter flow cytometry. In: Proceedings of the 13th International Cancer Congress. Part C; Biology of Cancer (2); E.A. Mirand, W.B. Hutchinson and E. Mihich (Eds.), Alan R. Liss, New York, pp. 171-177, 1983. 154. Staiano-Coico, L., Darzynkiewicz, Z., Hefton, J.M., Dutkowski, R., Darlington, G.J., and Weksler, M.: Increased sensitivity of lymphocytes from old humans to cell cycle arrest and chromosomal damage induced by 3H-TdR. Science, 219:1335-1337, 1983. 155. Darzynkiewicz, Z., Evenson, D., Kapuscinski, J., and Melamed, M.R.: Denaturation of RNA and DNA in situ induced by acridine orange. Exp. Cell Res., 148:31-46, 1983. 156. Kimmel, M., Traganos, F., and Darzynkiewicz, Z.: Do all daughter cells enter the "indeterminate" ("A") state of the cell cycle? Analysis of stathmokinetic experiments. Cytometry, 4:191-201, 1983. 157. Kapuscinski, J., and Darzynkiewicz, Z.: Increased accessibility of bases in DNA upon binding of acridine orange. Nucleic Acids Res., 11:7555-7568, 1983. 158. Kapuscinski, J., Darzynkiewicz, Z., and Melamed, M.R.: Interactions of acridine orange with nucleic acids. Properties of complexes of acridine orange with single stranded RNA. Biochem. Pharmacol., 32:3679-3694, 1983. 159. Gelfant, S., Drewinko, B., Darzynkiewicz, Z., Eisinger, M., and Camplejohn, R.S.: Cycling and noncycling human germinative epidermal cells. The continuous 3H-TdR labelling method in vivo and flow cytometric studies. In: Cell Proliferation and Psoriasis. Wright, N.A., and Camplejohn, R.S. (Eds.), Churchill Livingstone, London, pp. 209-217, 1983. 160. Higgins, P.J., Darzynkiewicz, Z., and Melamed, M.R.: Secretion of albumin and alpha fetoprotein by dimethylsulphoxide-stimulated hepatocellular carcinoma lines. Br. J. Cancer, 48:485-493, 1983. 161. Darzynkiewicz, Z.: Molecular interactions and cellular changes during the cell cycle. Pharmacol. Ther., 21:143-188, 1983. 1984 162. Higgins, P.J., Piwnicka, M., Darzynkiewicz, Z., and Melamed, M.R.: Multiparameter flow cytometric analysis of the regenerative response of mouse hepatocytes to carbon tetrachloride-induced liver necrosis. Am. J. Pathol., 115:31-35, 1984. 163. Darzynkiewicz, Z., Williamson, B., Carswell, E.A., and Old, L.J.: The cell cycle specific effects of tumor necrosis factor. Cancer Res., 44:83-90, 1984. 164. Traganos, F., Darzynkiewicz, Z., Bueti, C., and Melamed, M.R.: Effects of 1,4-bis(2'-chloroethyl)-1-4-diazabicyclo (2.2.1) heptane diperchlorate on cell survival and cell cycle progression of cultured mammalian cells. Cancer Invest., 2:1-13, 1984. 165. Darzynkiewicz, Z., and Carter, S.: Compound 48/80 impairs cytokinesis in murine leukemic cells. J. Cell Physiol., 119:1-6, 1984. 166. Darzynkiewicz, Z., Traganos, F., Kapuscinski, J., Staiano-Coico, L., and Melamed, M.R.: Accessibility of DNA in situ to various fluorochromes: Relationship to chromatin changes during erythroid differentiation of Friend leukemia cells. Cytometry, 5:355-363, 1984. 167. Staiano-Coico, L., Darzynkiewicz, Z., Melamed, M.R., and Weksler, M.: Immunological studies of aging. Impaired proliferation of T lymphocytes in elderly humans by flow cytometry. J. Immunol., 132:1788-1792, 1984. 168. Kapuscinski, J., and Darzynkiewicz, Z.: Denaturation of nucleic acids induced by intercalating agents. Biochemical and biophysical properties of acridine orange-DNA complexes. J. Biomol. Struct. Dyn., 1:1485-1499, 1984. 169. Traganos, F., Bueti, C., Darzynkiewicz, Z., and Melamed, M.R.: Effects of retinoic acid versus dimethylsulfoxide on Friend erythroleukemia cell growth. I. Cell proliferation, RNA and protein content. J. Natl. Cancer Inst., 72:193-204, 1984. 170. Traganos, F., Higgins, P.J., Bueti, C., Darzynkiewicz, Z., and Melamed, M.R.: Effects of retinoic acid versus dimethylsulfoxide on Friend erythroleukemia cells. II. Induction of quiescent, nonproliferating cells. J. Natl. Cancer Inst., 73:205-215, 1984. 171. Staiano-Coico, L., Wolf, R., Darzynkiewicz, Z., Whitmore, W.F., and Melamed, M.R.: RNA and DNA content of isolated nuclei from bladder irrigation specimens as measured by flow cytometry. Anal. Quant. Cytol., 6:24-29, 1984. 172. Czerniak, B., Darzynkiewicz, Z., Staiano-Coico, L., Herz, F., and Koss, L.G.: Expression of Ca antigen in relation to cell cycle of cultured human tumor cells. Cancer Res., 44:4342-4346, 1984. 173. Darzynkiewicz, Z., Carter, S., and Kimmel, M.: Effects of tritium labelled uridine on the cell cycle progression of L1210 cells. Cell Tissue Kinet., 17:641-655, 1984. 174. Kimmel, M., Darzynkiewicz, Z., Arino, O., and Traganos, F.: Metabolic cell cycle model based on unequal division of RNA to daughter cells during cytokinesis. J. Theor. Biol., 101:637-664, 1984. 175. Kapuscinski, J., and Darzynkiewicz, Z.: Condensation of nucleic acids by intercalating aromatic cations. Proc. Natl. Acad. Sci. USA, 81:7368-7372, 1984. 176. Walle, A., Suthanthivan, M., Taufield, P.A., Yankiwer, B., Rubin, A.L., Stenzel, K.A., and Darzynkiewicz, Z.: Multiparameter analysis of cell activation in renal allograft recipients. Transplant. Proc., 16:1585-1587, 1984. 1985 177. Darzynkiewicz, Z., Traganos, F., Kapuscinski, J., and Melamed, M.R.: Denaturation and condensation of DNA in situ induced by acridine orange. Relationship to chromatin structure of Friend erythroleukemia cells. Cytometry, 6:195-207, 1985. 178. Evenson, D.P., Lee, J.S., Darzynkiewicz, Z., and Melamed, M.R.: Rhodamine 123 alters the mitochondrial ultrastructure of cultured L1210 cells. J. Histochem. Cytochem., 33:353-359, 1985. 179. Crissman, H.A., Darzynkiewicz, Z., Tobey, R.A., and Steinkamp, J.A.: Correlated measurements of DNA, RNA and protein content in individual cells by flow cytometry. Science, 228:1321-1324, 1985. 180. Staiano-Coico, L., Wolf, R., Herr, H.W., Whitmore, W.F., Jr., Pinsky, C.M., Oettgen, H.F., Darzynkiewicz, Z., and Melamed, M.R.: Monitoring intravesicular BCG treatment of urinary bladder carcinoma with flow cytometry. J. Urol., 133:786-788, 1985. 181. Melamed, J., and Darzynkiewicz, Z.: RNA content and chromatin structure of CHO cells arrested in metaphase by Colcemid. Cytometry, 6:381-385, 1985. 182. Moran, R., Darzynkiewicz, Z., Staiano-Coico, L., and Melamed, M.R.: Detection of BrdUrd incorporation by monoclonal antibodies: Role of the DNA denaturation step. J. Histochem. Cytochem., 33:821-827, 1985. 183. Crissman, H.A., Darzynkiewicz, Z., Tobey, R.A., and Steinkamp, J.A.: Normal and perturbed growth of CHO cells. Correlated analysis of DNA, RNA and protein content by flow cytometry. J. Cell Biol., 101:141-147, 1985. 184. Staiano-Coico, L., Stollar, D., Darzynkiewicz, Z., Dutkowski, R., and Weksler, M.E.: Binding of anti-Z DNA antibodies in quiescent and activated lymphocytes: Relation to cell cycle progression and chromatin changes. Mol. Cell Biol., 5:3270-3273, 1985. 185. Kapuscinski, J., and Darzynkiewicz, Z.: Interactions of antitumor agents ametantrone and mitoxantrone (novantrone) with double-stranded DNA. Biochem. Pharmacol., 34:4203-4213, 1985. 186. Eisinger, M., Marko, O., Darzynkiewicz, Z., and Houghton, A.K.: Long-term cultures of normal human melanocytes in the presence of phorbol esters. Characterization of differentiated phenotype. In: Biological Molecular and Clinical Aspects of Pigmentation. Proc. XII Int. Pigment Cell Conference, Giessen, Germany. J. Bagnara and S.N. Klaus (Eds.). University of Tokyo Press, Tokyo, pp. 377-388, 1985. 1986 187. Evenson, D., Darzynkiewicz, Z., Jest, L., Janca, F., and Ballachey, B.: Changes in accessibility of DNA in situ to various fluorochromes during spermatogenesis. Cytometry, 7:45-53, 1986. 188. Staiano-Coico, L., Higgins, P.J., Darzynkiewicz, Z., Kimmel, M., Gottlieb, A.B., Pagan-Charry, Z., Madden, M.R., Finkelstein, J.L., and Hefton, J.M.: Human keratinocyte culture. Identification and staging of epidermal cell subpopulations. J. Clin. Invest., 77:396-404, 1986. 189. Melamed, M.R., Enker, W.E., Janov, A.J., Kessler, G., and Darzynkiewicz, Z.: Flow cytometry of colorectal carcinoma with three years follow-up. Col. Rec., 29:184-186, 1986. 190. Darzynkiewicz, Z., Traganos, F., and Staiano-Coico, L.: Cell and nuclear growth during G1: Kinetic and clinical implication. Ann. N.Y. Acad. Sci. ,468:45-54, 1986. 191. Kimmel, M., Darzynkiewicz, Z., and Staiano-Coico, L.: Stathmokinetic analysis of human epidermal cells in vitro. Cell Tissue Kinet., 19:289-304, 1986. 192. Kapuscinski, J., and Darzynkiewicz, Z.: Relationship between the pharmacological activity of antitumor drugs Ametantrone and Mitoxantrone (Novatrone) and their ability to condense nucleic acids. Proc. Natl. Acad. Sci. USA, 83:6302-6306, 1986. 193. Darzynkiewicz, Z., Kapuscinski, J., Carter, S.P., Schmid, F., and Melamed, M.R.: Cytostatic and cytotoxic properties of pyronin Y. Relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res., 46:5760-5766, 1986. 194. Darzynkiewicz, Z., Staiano-Coico, L., DeLeo, A.B., and Old, L.J.: p53 content in relation to cell growth, proliferation and RNA content in murine L1210 leukemia and normal thymocytes. Leukemia Res., 10:1383-1390, 1986. 195. Darzynkiewicz, Z.: Cell Growth and Division Cycle. Int. Encycl. Pharmacol. Ther., 121:1-43, 1986. 1987 196. Traganos, F., Bueti, C., Darzynkiewicz, Z., and Melamed, M.R.: Effects of a new amsacrine derivative, N-5 dimethyl 9((2-methoxy-4-methylsulfonylamino)phenyl-amino))-4-acridine carboxamide on cultured mammalian cells. Cancer Res., 47:424-432, 1987. 197. Darzynkiewicz, Z., Carter, S.P., and Old, L.J.: Effect of recombinant tumor necrosis factor on HL-60 cells. Cell cycle specificity and synergism with actinomycin D. J. Cell Physiol., 130:328-335, 1987. 198. Kapuscinski, J., and Darzynkiewicz, Z.: Interactions of pyronin Y (G) with nucleic acids. Cytometry, 8:129-137, 1987. 199. Darzynkiewicz, Z., Kapuscinski, J., Traganos, F., and Crissman, H.A.: Application of pyronin Y (G) in cytochemistry of nucleic acids. Cytometry, 8:138-145, 1987. 200. Cayre, Y., Wetzel-Raynal, M.C., Darzynkiewicz, Z., and Dorner, M.H.: A model for intermediate steps in monocytic differentiation: c-myc, c-fms and ferritin as markers. Proc. Natl. Acad. Sci. USA, 84:7619-7623, 1987. 201. Darzynkiewicz, Z., Traganos, F., Carter, S.P., and Higgins, P.J.: In situ factors affecting stability of the DNA helix in interphase nuclei and metaphase chromosome. Exp. Cell Res., 172:168-179, 1987. 202. Traganos, F., Bueti, C. Melamed, M.R., and Darzynkiewicz, Z.: Cytokinetic effects of the bi-functional antitumor intercalator-ditercalenium on Friend erythroleukemia cells. Leukemia, 1:411-416, 1987. 203. Kapuscinski, J., and Darzynkiewicz, Z.: Interactions of acridine orange with double stranded nucleic acids. Spectral and affinity studies. J. Biomol. Struct. Dyn., 5:127-142, 1987. 204. Kunicka, J.E., Darzynkiewicz, Z., and Melamed, M.R.: DNA in situ sensitivity to denaturation: A new parameter for flow cytometry of normal colonic epithelium and colon carcinoma. Cancer Res., 47:3942-3947, 1987. 205. Walle, A.J., Suthanthiran, M., Darzynkiewicz, Z., Rubin, A.L., and Stenzel, K.H.: Cell cycle analysis of activated mononuclear blood cells of renal allograft recipients. Transplant. Proc., 19:2183-2184, 1987. 206. Walle, A.J., Suthanthiran, M., Darzynkiewicz, Z., and Stenzel, K.H.: Nucleic acids content of blood mononuclear cells of renal allograft recipients. In: Clinical Cytometry and Histometry. Burger, G., Ploem, J.S., Goerttler, K., (Eds.), Academic Press, 1987, pp. 473-476. 207. Traganos, F., Kimmel, M., Bueti, C., and Darzynkiewicz, Z.: Effects of inhibition of RNA or protein synthesis on CHO cell cycle progression. J. Cell Physiol., 133:277, 1987. 208. Tobey, R.A., Crissman, H.A., Wilder, M.E., Traganos, F., and Darzynkiewicz, Z.: Sensitivity to X-irradiation in relation to cell size of CHO cells synchronized in early G1. Cell Tissue Kinet., 20:363-366, 1987. 1988 209. Walle, A.J., Wong, G.Y., Rubin, A.L., Stenzel, K.H., Suthanthiran, M., and Darzynkiewicz, Z.: Altered nucleic acid content of mononuclear cells in blood of renal allograft recipients. Cytometry, 9:177-182, 1988. 210. Darzynkiewicz, Z., and Kapuscinski, J.: Condensation of DNA in situ in metaphase chromosomes induced by intercalating ligands and its relationship to chromosome binding. Cytometry, 9:7-18, 1988. 211. Darzynkiewicz, Z., and Carter, S.P.: Photosensitizing effects of the tricyclic heteroaromatic cationic dyes pyronin Y and toluidine blue O (tolonium chloride). Cancer Res., 48:1295-1299, 1988. 212. Traganos, F., Crissman, H.A., and Darzynkiewicz, Z.: Staining with pyronin Y detects changes in conformation of RNA during mitosis and hyperthermia of CHO cells. Exp. Cell Res., 179:535-544, 1988. 213. Kapuscinski, J., Traganos, F., and Darzynkiewicz, Z.: Interactions of antitumor drug ditercalinium with nucleic acids in solutions and in situ. Cancer Lett., 42:185-191, 1988. 214. Darzynkiewicz, Z.: Flow cytometry in cytopathology - Overview and perspectives. Anal. Quant. Cytol. Histol., 10:459-461, 1988. 215. Darzynkiewicz, Z., and Traganos, F.: Unstainable DNA in cell nuclei. Comparison of ten different fluorochromes. Anal. Quant. Cytol. Histol., 10:462-466, 1988. 216. Darzynkiewicz, Z., Carter, S.P., Mikulski, S.M., and Shogen K.: Cytostatic and cytotoxic effects of Pannon, a novel anticancer agent. Cell Tissue Kinet. 21:169-182, 1988. 217. Darzynkiewicz, Z.: Cellular RNA content, a feature correlated with cell kinetics and tumor prognosis. Leukemia, 2:777-787, 1988. 1989 218. Darzynkiewicz, Z., and Carter, S.P.: Thermal stability of nucleosomes studied in situ by flow cytometry. Effect of ionic strength and n-butyrate. Exp. Cell Res., 180:551-556, 1989. 219. Traganos, F., Bueti, C., Kapuscinski, J., and Darzynkiewicz, Z.: The antitumor intercalating drug ditercalinium binds preferentially to RNA in Friend erythroleukemia cells. Leukemia, 3:522-529, 1989. 220. Myc, A., Kunicka, J.A., Melamed, M.R., and Darzynkiewicz, Z.: Effect of swainsonine on stimulation and cell cycle progression of human lymphocytes. Cancer Res., 49:2879- 2883, 1989. 221. Bartkowiak, J., Kapuscinski, J., Melamed, M.R., and Darzynkiewicz Z.: Selective displacement of nuclear proteins by antitumor drugs having affinity to nucleic acids. Proc. Natl. Acad. Sci. USA, 86: 5151-5154, 1989. 222. Koyama, M., Takahashi, K., Chou, T-C., Darzynkiewicz, Z., Kapuscinski, J., Kelly, T.R., and Watanabe, K.A.: Intercalating agents with covalent bond forming capability. Novel type of potential anticancer agents. 2. Derivatives of chrysophanol and emodin. J. Med. Chem., 32: 1594-1599, 1989. 223. Darzynkiewicz, Z., Carter, S.P., Kapuscinski, J., and Watanabe, K.A.: Effect of derivatives of chrysophanol, a new type of potential antitumor agent of anthraquinone family on growth and cell cycle of L1210 leukemic cells. Cancer Lett., 46:181-187, 1989. 224. Kunicka, J.E., Olszewski, W., Rosen, P., Kimmel, M., Melamed, M.R., and Darzynkiewicz, Z.: DNA in situ sensitivity to denaturation as a marker of human breast tumors. Cancer Res., 49: 6347-6351, 1989. 225. Bories, D., Raynal, M.-C., Solomon, D.H., Darzynkiewicz, Z., and Cayre, Y.E.: Down-regulation of a serine protease, Myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells. Cell, 59: 959-968, 1989. 226. Staiano-Coico, L., Darzynkiewicz, Z., and McMahon, C.: Cultured human keratinocytes: Discrimination of different cell cycle compartments based upon measurement of nuclear RNA or total cellular RNA content. Cell Tissue Kinet., 22: 235-243, 1989. 227. Czerniak, B., Darzynkiewicz, Z., Herz, F., Wersto, R.P., and Koss, L.G.: Flow cytometry in clinical oncology: Cell cycle and DNA ploidy in assessing tumor behavior. Mater. Med. Pol., 21:3-9, 1989. 1990 228. Kunicka, J.E., Myc, A., Melamed, M.R., and Darzynkiewicz, Z.: Caffeine affects the cell cycle progression and decreases stability of DNA helix in situ in L1210 cells. Cell Tissue Kinet., 23:31-39, 1990. 229. Kapuscinski, J., and Darzynkiewicz, Z.: Structure destabilization and condensation of nucleic acids by intercalators. Structure & Methods, Vol. 3: DNA & RNA. In: Proceedings of the 6th Conversation in the Discipline Biomolecular Stereodynamics. R.H Sarma, and M.H.Sarma, (Eds.), Adenine Press, Schenectady, N.Y., 1990, pp. 267-281. 230. Evenson, D., and Darzynkiewicz, Z.: Acridine orange induced precipitation of mouse testicular sperm cell DNA reveals new patterns of chromatin structure. Exp. Cell Res., 187:328-334, 1990. 231. Del Bino, G., Skierski, J.S., and Darzynkiewicz, Z.: Diverse effects of camptothecin, an inhibitor of topoisomerase I on the cell cycle of lymphocytic (L1210, MOLT-4) and myelogenous (HL-60, KG1) leukemic cells. Cancer Res., 50:5746-5750, 1990. 232. Myc, A., DeAngelis, P., Melamed, M.R., and Darzynkiewicz, Z.: Retention of the mitochondrial probe rhodamine 123 in normal lymphocytes and leukemic cells in relation to the cell cycle. Exp. Cell Res., 192:198-202, 1990. 1991 233. Del Bino, G., Lassota, P., and Darzynkiewicz, Z.: The S-phase cytotoxicity of camptothecin. Exp. Cell Res. 294:27-35, 1991. 234. Lassota, P., Melamed, M.R., and Darzynkiewicz, Z.: Release of specific proteins from nuclei of HL-60 and MOLT-4 cells by antitumor drugs having affinity to nucleic acids. Biochem. Pharmacol. 41:1055-1065, 1991. 235. Bretton P.R., Darzynkiewicz, Z., Henry, E., Kimmel, M., Fair, W.R., and Melamed, M.R.: DNA in situ sensitivity to denaturation in bladder cancer and its correlation with tumor stage. Cancer Res. 50:7912-7914, 1991. 236. Del Bino, G., and Darzynkiewicz, Z.: Camptothecin, teniposide or m-AMSA but not mitoxantrone or doxorubicin induce degradation of nuclear DNA in S-phase of HL-60 cells. Cancer Res. 51:1165-1169, 1991. 237. Myc, A., DeAngelis, P., Melamed, M.R., and Darzynkiewicz, Z.: Effect of Swainsonine on Il-2 receptor expression and proliferation of human lymphocytes. Clin. Exp. Immunol., 4:406-410, 1991. 238. Traganos, F., Kapuscinski, J., and Darzynkiewicz, Z.: Caffeine modulates the effects of DNA intercalating drugs in vitro: A flow cytometric and spectrophotometric analysis of caffeine interaction with Novantrone, doxorubicin, ellipticine and the doxorubicin analog, AD198. Cancer Res., 51:3682-3689, 1991. 239. Traganos, F., Kaminska-Eddy, B., and Darzynkiewicz, Z.: Caffeine reverses the cytotoxic and cell kinetic effects of Novantrone (Mitoxantrone). Cell Prolif., 24:305-319, 1991. 240. Bruno, S., Bauer, K., Crissman, H.A., and Darzynkiewicz, Z.: Changes in cell nuclei during S phase: Progressive chromatin condensation and altered expression of the proliferation-associated nuclear proteins Ki-67, p-105, cyclin/PCNA and p34. Exp. Cell Res., 196:99-106, 1991. 241. Jakóbisiak, M., Bruno, S., Skierski, J., and Darzynkiewicz, Z.: The cell cycle specific effects of lovastatin. Proc. Natl. Acad. Sci. USA, 88:3628-3632, 1991. 242. Strife, A., Lambek, C., Perez, A., Darzynkiewicz, Z., Skierski, J., Gulati, S., Haley, J.D., Iwata, K.K., and Clarkson, B.D.: The effects of TGF-alpha on the growth of highly enriched hematopoietic progenitor cells derived from normal human bone marrow and peripheral blood. Cancer Res., 51:4828-4836, 1991. 243. Del Bino, G., Skierski, J., and Darzynkiewicz, Z.: The concentration-dependent diversity of effects of DNA topoisomerase I and II inhibitors on the cell cycle of HL-60 cells. Exp. Cell Res., 195:485-491, 1991. 1992 244. Bruno, S., Ardelt, B., Skierski, J.S., Traganos, F., and Darzynkiewicz, Z.: Different effects of staurosporine, an inhibitor of protein kinases, on the cell cycle and chromatin structure of normal and leukemic lymphocytes. Cancer Res., 52:470-473, 1992. 245. Bruno, S., and Darzynkiewicz, Z.: Cell cycle dependent expression and stability of the nuclear protein detected by Ki-67 antibody in HL-60 cells. Cell Prolif., 25:31-40, 1992. 246. Rivas, C.I., Wisniewski, D., Strife, A., Perez, A., Lambek, C., Bruno, S., Darzynkiewicz, Z., and Clarkson, B.: Constitutive expression of p53 protein in enriched normal human marrow blast cell populations. Blood, 79:1982-1986, 1992. 247. Hotz, M.A., Del Bino, G., Lassota, P., Traganos, F., and Darzynkiewicz, Z.: Cytostatic and cytotoxic effects of the DNA topoisomerase II inhibitor, fostriecin, on human promyelocytic HL-60 and lymphocytic MOLT-4 leukemic cells. Cancer Res., 52:1530-1535, 1992. 248. Myc, A., Traganos, F., Lara, J., Melamed, M.R., Darzynkiewicz, Z.: DNA stainability in aneuploid breast tumors: comparison of four DNA fluorochromes differing in binding properties. Cytometry, 13:389-394, 1992. 249. Bruno, S., Giaretti, W., Darzynkiewicz, Z.: Effect of camptothecin on mitogenic stimulation of human lymphocytes: Involvement of DNA topoisomerase I in cell transition from G0 to G1 phase of the cell cycle and in DNA replication. J. Cell Physiol., 151:478-486, 1992. 250. Bruno, S., Gorczyca, W., and Darzynkiewicz, Z.: The effect of ionic strength in immunocytochemical detection of the proliferation associated nuclear antigens p120, PCNA and the protein reacting with Ki-67 antibody. Cytometry, 12:496-501, 1992. 251. Hotz, M.A., Traganos, F., and Darzynkiewicz, Z.: Changes in nuclear chromatin related to apoptosis or necrosis induced by the DNA topoisomerase II inhibitor fostriecin in MOLT-4 and HL-60 cells are revealed by altered DNA sensitivity to denaturation. Exp. Cell Res., 201:184-191, 1992. 252. Mikulski, S.M., Viera, A., and Darzynkiewicz, Z.: Synergism between a novel amphibian oocyte ribonuclease and lovastatin in inducing cytostatic and cytotoxic effects in human lung and pancreatic carcinoma cell lines. Br. J. Cancer, 66:304-310, 1992. 253. Bruno, S., Lassota, P., Giaretti, W., Darzynkiewicz, Z.: Apoptosis of rat thymocytes triggered by prednisolone, camptothecin, or teniposide is selective to G0 cells and is prevented by inhibitors of proteases. Onc. Res., 4:29-35, 1992. 254. Gorczyca, W., Bruno, S., Darzynkiewicz, R.J., Gong, J., and Darzynkiewicz, Z.: DNA strand breaks occurring during apoptosis: Their early in situ detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors. Intl. J. Onc., 1:639-648, 1992. 255. Darzynkiewicz, Z., Bruno, S., Del Bino, G., Gorczyca, W., Hotz, M.A., Lassota, P., and Traganos, F.: Features of apoptotic cells measured by flow cytometry. Cytometry, 13:795-808, 1992. 256. Bruno, S., Del Bino, G., Lassota, P., Giaretti, W., and Darzynkiewicz, Z.: Inhibitors of proteases prevent endonucleolysis accompanying apoptotic cell death. Leukemia, 6:1113-1120, 1992. 257. Del Bino, G., Bruno, S., Yi, P.N., Darzynkiewicz, Z.: Apoptotic cell death triggered by camptothecin or teniposide: The cell cycle specificity and effects of ionizing radiation. Cell Prolif., 25:537-548, 1992. 258. Black, R.R., Bouvier, M.M., Sheu,K-F.R., Darzynkiewicz, Z. and Blass, J.P.: Presence of typical neuronal markers in serially cultured cells from adult human brain. J. Neurol. Sci., 111:104-112, 1992. 259. Traganos, F., Ardelt, B., Halko, N.M., Bruno, S., and Darzynkiewicz, Z.: Effect of Genistein, a tyrosine kinase inhibitor, on the growth and cell cycle progression of normal human lymphocytes and human leukemic MOLT-4 and HL-60 cells. Cancer Res., 52: 6200-6208, 1992. 1993 260. Traganos, F., Knutti-Hotz, J., Hotz, M., Gorczyca, W., Ardelt, B., and Darzynkiewicz, Z.: The protein kinase C inhibitor H7 blocks normal human lymphocyte stimulation and induces apoptosis of both normal lymphocytes and leukemic MOLT-4 cells. Intl. J. Onc., 2:47-59, 1993. 261. Strife, A., Perez, A., Lambek, C., Wisniewski, D., Bruno, S., Darzynkiewicz, Z., and Clarkson, B. Characterization of lineage-negative blast subpopulations derived from normal and chronic myelogenous leukemia bone marrows and determination of their responsiveness to human c-kit ligand. Cancer. Res., 53:401-409, 1993. 262. Capasso, J.M., Bruno, S., Zhang, X., Darzynkiewicz, Z. and Anversa, P.: Myocyte DNA synthesis with aging: correlation with ventricular loading in rats. J. Cell Phys., 155:635-648, 1993. 263. Capasso, J.M., Bruno, S., Cheng, W., Li, P., Rodgers, R., Darzynkiewicz, Z., and Anversa, P.: Ventricular loading is coupled with DNA synthesis in adult cardiac myocytes after acute and chronic myocardial infarction in rats. Circulation, 71: 1379-1389, 1993. 264. Darzynkiewicz, Z., Gorczyca, W., Lassota, P., and Traganos, F.: Altered sensitivity of DNA in situ to denaturation in apoptotic cells. Annal. N.Y. Acad. Sci., 677:334-339, 1993. 265. Gorczyca, W., Melamed, M.R., Darzynkiewicz, Z.: Apoptosis of S phase HL-60 cells induced by DNA topoisomerase inhibitors: Detection of DNA strand breaks by flow cytometry using the in situ nick translation assay. Toxic. Lett., 67:249-258, 1993. 266. Gorczyca, W., Gong, J., and Darzynkiewicz, Z.: Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res., 53:1945-1951, 1993. 267. Darzynkiewicz, Z., and Melamed, M.R.: Flow cytometry and sorting: An historical perspective. Cytometry Res., 3:1-9, 1993. 268. Gorczyca, W., Gong, J., Ardelt, B., Traganos, F., Darzynkiewicz, Z.: The cell cycle related differences in susceptibility of HL-60 cells to apoptosis induced by various antitumor agents. Cancer Res., 53:3186-3192, 1993. 269. Gorczyca, W., Bigman, K., Mittelman, A., Ahmed T., Gong J., Melamed, M.R., Darzynkiewicz, Z.: Induction of DNA strand breaks associated with apoptosis during treatment of leukemias. Leukemia, (Baltimore) 7: 659-670, 1993. 270. Gorczyca, W., Traganos, F., Jesionowska, H., Darzynkiewicz, Z.: Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells. Exp. Cell Res., 207:202-205, 1993. 271. Gorczyca, W., Ardelt, B., Darzynkiewicz, Z.: Different rates of degradation of the proliferationo-associated nuclear and nucleolar antigens during apoptosis of HL-60 cells induced by DNA topoisomerase inhibitors. Int. J. Onc., 3: 627-634, 1993. 272. Gong, J., Traganos, F., Darzynkiewicz, Z.: Simultaneous analysis of cell cycle kinetics at two different DNA ploidy levels based on DNA content and cyclin B measurements. Cancer Res., 53:5096-5099, 1993. 273. Traganos, F., Kapuscinski, J., Gong, J., Ardelt, B., Darzynkiewicz, R.J., and Darzynkiewicz, Z.: Caffeine prevents apoptosis and cell cycle effects induced by camptothecin or topotecan in HL-60 cells. Cancer Res., 53: 4613-4618, 1993. 274. Strife, A., Perez, A., Lambek, C., Wisniewski, D., Bruno, S., Darzynkiewicz, Z., and Clarkson B.: Differences in the composition and in the efficiency of red cell production of normal and CML erythroid progenitor populations are highlighted by response to human c-kit ligand. Leukemia Res., 17:799-807, 1993. 275. Gong, J., Li. X. and Darzynkiewicz Z: Different patterns of apoptosis of HL-60 cells induced by cycloheximide and camptothecin. J. Cell Physiol., 157: 263-270, 1993. 276. Gorczyca, W., Melamed, M.R. and Darzynkiewicz, Z.: Programmed cell death (apoptosis). Patol. Pol., 44: 113-119, 1993. 277. Gorczyca, W., Kram, A., Tuziak, T., Darzynkiewicz, Z., Melamed, M.R.:Proliferation Cell Nuclear Antigen in archival surgical specimens of malignant lymphoma and metastatic carcinoma: Immunohistochemical and flow cytometric analysis. Pat. Pol., 44:121-128, 1993. 278. Gong, J., Traganos, F., and Darzynkiewicz, Z.: Expression of cyclins B and E in individual MOLT-4 cells and in stimulated human lymphocytes during their progression through the cell cycle. Int. J. Onc., 3:1037-1042,1993. 1994 279. Blass, J.P., Markersbury, W.R., Ko, L.W., De Giorgio, L., Sheu, K.F. and Darzynkiewicz, Z.: Presence of neuronal proteins in serially cultured cells from autopsy human brain. J. Neurol. Sci., 121: 132-138, 1994. 280. Del Bino, G., Li, X., Traganos, F., Darzynkiewicz, Z.: Altered susceptibility of differentiating HL-60 cells to apoptosis induced by antitumor drugs. Leukemia, 6: 281-288, 1994. 281. Gorczyca, W., Tuziak, T., Kram, A., Melamed, M.R., and Darzynkiewicz, Z.: Detection of apoptosis in fine-needle aspiration biopsies by in situ end-labeling of fragmented DNA. Cytometry, 15: 169-175,1994. 282. Gong, J., Traganos, F. and Darzynkiewicz, Z.: Use of cyclin E restriction point to map cell arrest in G1 induced by n-butyrate, cycloheximide, staurosporine, lovastatin, mimosine and quercetin. Int. J. Oncol., 4: 803-808, 1994. 283. Garcia-Valenzuela, E., Gorczyca, W., Darzynkiewicz, Z., Sharma, S.C.: Apoptosis in adult retinal ganglion cells after axotomy. J. Neurobiol., 25: 431-438, 1994. 284. Hotz, M.A., Gong, J., Traganos, F., and Darzynkiewicz, Z.: Flow cytometric detection of apoptosis. Comparison of the assays of in situ DNA degradation and chromatin changes. Cytometry, 15:237-244, 1994. 285. Gong, J., Traganos, F. and Darzynkiewicz, Z.: A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal. Biochem., 218:314-319, 1994. 286. Li, X., Gong, J., Feldman, E., Seiter, K., Traganos, F. and Darzynkiewicz, Z.: Apoptotic cell death during treatment of leukemias. Leukemia and Lymphoma, 13: Suppl. 1, 65-70, 1994. 287. Lassota, P., Kazimierczuk, Z.,and Darzynkiewicz, Z.: Apoptotic death of lymphocytes upon treatment with 2-chloro-2'-deoxyadenosine (2-CdA). Arch. Immunol. & Exp. Ther., 42: 17-23, 1994. 288. Traganos, F., Gong, J., Ardelt, B., Darzynkiewicz, Z.: Effect of staurosporine on MOLT-4 cell progression through G2 and on cytokinesis. J. Cell Phys., 158: 535-544, 1994. 289. Gong, J., Traganos, F. and Darzynkiewicz, Z.: Staurosporine blocks cell progression through G1 between cyclin D and cyclin E restriction points. Cancer Res., 54: 3136-3139,1994. 290. Li, X., Traganos, F., Melamed, M.R. and Darzynkiewicz, Z.: Detection of 5-bromo-2-deoxyuridine incorporated into DNA by labeling strand breaks induced by photolysis (SBIP). Int. J. Oncol., 4:1157-1161, 1994. 291. Gong,., Ardelt, B., Traganos, F. and Darzynkiewicz, Z.:Unscheduled expression of cyclin B1 and cyclin E in several leukemic and solid tumor cell lines. Cancer Res., 54: 4285-4288, 1994. 292. Li, X., Traganos, F. and Darzynkiewicz, Z.: Simultaneous analysis of DNA replication and apoptosis during treatment of HL-60 cells with camptothecin and hyperthermia and mitogen stimulation of human lymphocytes. Cancer Res., 54: 4289-4293, 1994. 293. Li, X., Patel, B., Melamed, M.R., Darzynkiewicz, Z.: The cell cycle effects and induction of apoptosis by 5-bromouridine in cultures of human leukemic MOLT-4 and HL-60 cell lines and mitogen stimulated normal lymphocytes, Cell Prolif., 27: 307-319, 1994. 294. Kajstura, J., Zhang, X., Reiss, K., Szoke, E., Li, P., Lagrasta, C., Cheng, W., Darzynkiewicz, Z., Olivetti, G., Anversa, P.: Myocyte cellular hyperplasia and myocyte cellular hypertrophy contribute to chronic ventricular remodeling in coronary artery narrowing induced cardiomyopathy in rats. Circulation Res, 74: 383-400, 1994. 295. Black, R.S., DeGiorgio, L.A., Sheu, K-F.R., Darzynkiewicz, Z., Duffy, J.T. and Blass, J.P.: Expression of neuronal proteins in cells from normal adult brain propagated in serial culture. J. Neurochemistry. 62: 2132-2140, 1994. 296. Gong, J., Li, X., Traganos, F. and Darzynkiewicz, Z.: Expression of G1 and G2 cyclins measured in individual cells by multiparameter flow cytometry; a new tool in the analysis of the cell cycle. Cell Prolif., 27: 357-371, 1994. 297. Wang, Z., Dudhane, A., Orlikowsky, T., Clarke, K., Li, X., Darzynkiewicz, Z. and Hoffman, M.K.: CD4 engagement in vivo induces apoptosis in T cells. Eur. J. Immunol., 24: 1549-1552, 1994. 1995 298. Halicka, D.H., Ardelt, B., Li, X., Melamed, M.R, and Darzynkiewicz, Z.:2-deoxyglucose enhances sensitivity of human histiocytic U 935 cells to apoptosis induced by Tumor Necrosis Factor. Cancer Res., 55: 444-449, 1995. 299. Gong, J., Traganos, F. and Darzynkiewicz, Z.: Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry. Leukemia, 9: 893-899, 1995. 300. Li, X., Traganos, F., Melamed M.R. and Darzynkiewicz, Z.: Single step procedure for DNA strand break labeling. Detection of apoptosis and DNA replication. Cytometry, 20: 172-180, 1995. 301. Bhatia, U., Danishefsky, K.,Traganos, F. and Darzynkiewicz, Z.: Induction of apoptosis and cell cycle specific change in expression of p53 in normal lymphocytes and MOLT-4 leukemic cells by nitrogen mustard. Clinical Cancer Res., 1: 873-880, 1995. 302. Li, X., James, W.M., Traganos, F., and Darzynkiewicz, Z.: Application of biotin, digoxygenin or fluorescein conjugated deoxynucleotides to label DNA strand breaks in analysis of cell proliferation and apoptosis by flow cytometry. Biotech. Histochem., 70: 235-242, 1995. 303. Qiao, L., Gorczyca, W., Pizzolo, J.G., Melamed, M.R. and Darzynkiewicz, Z.: Flow cytometric characterization of proliferation-associated nuclear antigen (p105) during the cell cycle in normal lymphocytes and promyelocytic leukemia cells. Anal. Quant. Cytol. Histol.,17: 183-188, 1995. 304. Darzynkiewicz, Z.: Apoptosis in antitumor strategies: Modulation of cell cycle or differentiation. J. Cell Biochem., 58: 151-159, 1995. 305. Murakami, T., Li, X., Gong, J., Bhatia U., Traganos, F. and Darzynkiewicz, Z.: Induction of apoptosis by 5-azacytidine. Drug concentration dependent differences in cell cycle specificity. Cancer Res., 55: 3093-3098, 1995. (36) 306. Gong, J., Traganos, F. and Darzynkiewicz, Z.: Threshold expression of cyclin E but not D type cyclins characterizes normal and tumor cells entering S phase. Cell Proliferation, 28: 337-346, 1995. 307. Bhatia, U., Traganos, F., and Darzynkiewicz, Z.: Induction of cell differentiation potentiates apoptosis triggered by prior exposure to DNA damaging drugs. Cell Growth & Differentiation, 6: 937-944, 1995. 308. Gong, J., Traganos, F. and Darzynkiewicz, Z.: Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins B1 and A. Exp. Cell Res., 220: 226-231, 1995. 309. Venturelli, D., Martinez, R., Melotti, P., Casella, I., Peschle, C., Cucco C., Spaminato G., Darzynkiewicz, Z., and Calabretta B.: Overexpression of DR-nm23, a protein encoded by a member of nm23 family, inhibits granulocyte differentiation and induces apoptosis in 32Dc13 myeloid cells. Proc. Natl. Acad. Sci. USA, 92: 7435-7439, 1995. 310. Gong, J., Traganos, F. and Darzynkiewicz, Z.: Growth imbalance and altered expression of cyclins B1, A. E and D3 in MOLT-4 cells synchronized in the cell cycle by inhibitors of DNA replication. Cell Growth & Differentiation, 6: 1485-1493, 1995. 311. Ronai, Z., Tillotson, J.K., Traganos, F., Darzynkiewicz, Z., Conaway, C., Upadhyaya,P., and El-Bayoumy, K.: Effects of organic and inorganic selenium compounds on rat mammary tumor cells. Int. J. Cancer., 63: 428-434, 1995. (42) 312. Li, X. and Darzynkiewicz, Z.: Labeling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation. Cell Proliferation, 28: 571-579, 1995. 313. Hara S., Tomita M. and Darzynkiewicz, Z.: A flow cytometric study of correlation between heat induced apoptosis and heat shock protein 70 in HL-60 cells. Cytometry Res., 5: 83-88, 1995. 314. Seiter, K., Feldman, E.J., Traganos, F., Li, X., Halicka, H.D., Darzynkiewicz, Z., Lederman, C.A., Romero, M.-B. and Ahmed, T.: Phase I study on Taxol in acute leukemis: Evaluation of in vivo induction of apoptosis by Taxol. Leukemia, 9: 1961-1963, 1995. 315. Skorski, T., Nieborowska-Skorska, M., Campbell, K., Iozzo, R.V., Zon, G., Darzynkiewicz, Z. and Calabretta, B.: Leukemia treatment in severe combined immunodeficiency mice by antisense oligonucleotides targeting cooperating oncogenes. J. Exp. Med., 182: 1645-1653, 1995 (46) 316. Darzynkiewicz, Z., Hara, S., Murakami, T., Numa, Y. and Li, X.: Flow cytometric analysis of cell death: A critical review. Cytometry Research., 5: 1-8, 1995. 1996 317. Li, X., Melamed, M.R. and Darzynkiewicz, Z.: Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color. Exp. Cell Res., 222: 28-37, 1996. 318. Hara,S., Halicka, H.D., Bruno, S., Gong, J., Traganos, F. and Darzynkiewicz, Z.: Effect of protease inhibitors on early events of apoptosis. Exp. Cell Res., 223: 372-384, 1996. 319. Juan, G., Gong, J., Traganos, F., and Darzynkiewicz, Z.: Unscheduled expression of cyclins D1 and D3 in human tumor cell lines. Cell Proliferation, 29, 259-266, 1996. 320. Juan, G., Pan, W., and Darzynkiewicz, Z.:DNA segments sensitive to single strand specific nucleases are present in chromatin of mitotic cells. Exp.Cell Res., 227: 197-202, 1996. 321. Bruno, S., Traganos, F. and Darzynkiewicz, Z.: Cell cycle synchronizing properties of staurosporine. Meth. Cell Sci., 18: 99-107, 1996. 322. Darzynkiewicz, Z., Gong, J., Juan, G., Ardelt, B and Traganos, F.: Cytometry of cyclin proteins. Cytometry, 25: 1-13, 1996. 323. Darzynkiewicz, Z., Bruno, S., Del Bino, G., and Traganos, F.: The cell cycle effects of camptothecin. In: The Camptothecins. From Discovery to the Patient. P. Pantazis, B.C.Giovanella, M.L.Rothenberg, Eds. Ann. N.Y. Acad. Sci. 803: 93-100, 1996. 324. Traganos, F., Seiter, K., Halicka, H.D., and Darzynkiewicz, Z.: Induction of apoptosis by camptothecin and topotecan.In: The Camptothecins. From Discovery to the Patient. P. Pantazis, B.C.Giovanella, M.L.Rothenberg, Eds. Ann. N.Y. Acad. Sci. 803: 101-110, 1996. 325. Gorczyca, W., Melamed, M.R.and Darzynkiewicz, Z.: Laser scanning cytometer analysis of fraction of labeled mitoses. Cell Proliferation, 29: 539-548, 1996. 326. Numa, Y., Matsudaira T., Kawamoto, K and Darzynkiewicz, Z. Induction of apoptosis in HL-60 (Human Leukemia-60) cells by MX-2 (new morpholino anthracyclin). Cytometry Res.,(Japan) 6: 65-70, 1996. 327. Darzynkiewicz, Z.: Flow cytometric detection of apoptosis and necrosis. In: Proceedings of the Xth International Congress of Histochemistry and Cytochemistry. Kyoto, Japan. Acta Histochemica Cytochemica., Vol 29. Supplement. pp. 46-47. 1996. 328. Murakami, T., Sasaki, K., Traganos, F. and Darzynkiewicz, Z.: Flow cytometric and laser scanning cytometric analysis of apoptosis induced by 5'azacytidine. In: Proceedings of the Xth International Congress of Histochemistry and Cytochemistry. Kyoto, Japan. Acta Histochemica Cytochemica. Vol 29. Supplement. pp. 48-49. 1996. 1997 329. Seiter K., Feldman, E. J., Halicka, D., Traganos, F., Darzynkiewicz, Z., Lake, D., and Ahmed, T.: Phase I clinical and laboratory evaluation of topotecan and cytoarabine in patients with acute leukemia. J. Clin. Oncol., 15: 44-55, 1997. 330. Vibhakar, R., Juan, G., Traganos, F., Darzynkiewicz, Z., and Finger, L.: Activation induced expression of human programmed death-1 gene in T-lymphocytes. Exp. Cell Res., 232: 25-28, 1997. 331. Darzynkiewicz, Z., Juan, G., Li, X., Gorczyca, W., Murakami, T. and Traganos, F.: Cytometry in cell necrobiology. Analysis of apoptosis and accidental cell death (necrosis). Cytometry, 27: 1-20, 1997. 332. Gorczyca, W., Darzynkiewicz, Z. and Melamed MR: Laser scanning cytometry in pathology of solid tumors. Acta Cytologica, 41: 98-108, 1997. 333. Halicka H.D., Seiter, K., Feldman, E.J., Traganos, F., Mittelman, A., Ahmed, T., and Darzynkiewicz, Z.: Cell cycle specificity of apoptosis during treatment of leukemias. Apoptosis, 2: 25-39, 1997. 334. Juan, G., Li, X., and Darzynkiewicz, Z.: Correlation between DNA replication and expression of cyclins A and B1 in individual MOLT-4 cells. Cancer Res., 57: 803-807, 1997. 335. Haider, S. R., Juan, G., Traganos, F., and Darzynkiewicz, Z.: Immuno-separation and immuno-detection of nucleic acids with halogenated nucleotides. Exp. Cell Res., 234: 498-506, 1997. 336. Halicka, H.D., Ardelt, B., Juan, G., Mittelman, A., Chen, S., Traganos, F., and Darzynkiewicz, Z. Apoptosis and cell cycle effects induced by extracts of the Chinese herbal preparation PC SPES. Int. J. Oncol., 11: 437-448, 1997. 337. Gorczyca, W., Sorano, V., Juan, G., Melamed, M.R., and Darzynkiewicz, Z.: Laser scanning analysis of cyclin B1 in primary human malignancies. Modern Pathol., 10: 457-462, 1997 338. Darzynkiewicz, Z.: Induction of apoptosis during treatment of leukemias. Relationship to the cell cycle. Acta Haematol. Pol., 28, Suppl. 2, 9-21. 1997 339. Bedner, E., Burfeind, P., Gorczyca, W., Melamed, M.R., and Darzynkiewicz, Z.: Laser scanning cytometry distinguishes lymphocytes, monocytes and granulocytes by differences in their chromatin structure. Cytometry, 29: 191-196. 1997. 340 Sailer, B.L., Valdez, J.G., Steinkamp, J.A., Darzynkiewicz, Z., and Crissman,. H.A.: Monitoring uptake of ellipticin and its fluorescence lifetime in relation to the cell cycle phase by flow cytometry. Exp. Cell Res., 236: 259-267, 1997. 1998 341. Deptala, A., Halicka, H.D., Ardelt, B., Ardelt, W., Mikulski, S.M., Shogen, K., and Darzynkiewicz, Z.: Potentiation of tumor necrosis factor induced apoptosis by onconase. Int. J. Oncol., 13: 11-16, 1998 342. Juan, G., Traganos, F., James, W.M., Ray, J.M., Roberge, M., Sauve, D.M., Anderson, H, and Darzynkiewicz, Z.: Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis. Cytometry, 32: 71-77, 1998. 343. Juan, G., Gruenwald, S., and Darzynkiewicz, Z.: Phosphorylation of retinoblastoma susceptibility gene protein assayed in individual lymphocytes during their mitogenic stimulation. Exp. Cell Res., 239: 104-110, 1998. 344. Hara, S., Tagawa, Y., Ayabe, H., and Darzynkiewicz, Z.: Analysis of of drug-induced apoptosis in human leukemic cell line HL-60. Gan. To. Kagaku Ryoho. (Jap) Suppl. 3: 393-399, 1998. 345. Bedner, E., Burfeind, Hsieh,T-C, Wu, J.M, Aguero-Rosenfeld, M.A., Melamed, M.R., Horowitz, H.W., Wormser, G.P., and Darzynkiewicz, Z.: Cell cycle effects and induction of apoptosis caused by infection of HL-60 cells with human granulocytic ehrlichiosis (HGE) pathogen measured by flow and laser scanning cytometry (LSC). Cytometry, 33: 47-55, 1998. 346. Bedner, E., Melamed, M.R., and Darzynkiewicz, Z: Time resolved kinetic reactions measured in individual cells by laser scanning cytometry (LSC) Cytometry, 33: 1-9, 1998. 347. Juan, G., Ardelt, B., Mikulski, S.M., Shogen, K,M Ardelt, W., Mittelman, A., and Darzynkiewicz, Z.: G1 arrest of U-937 cells by onconase is associated with suppression of cyclin D3 expression, induction of p16INK4A, p21WAF1/CIP1 and p27KIP and decreased pRb phosphorylation. Leukemia, 12:1241-1248, 1998. 348. Darzynkiewicz, Z., Bedner, E., Traganos, F., and Murakami T.: Critical aspects in analysis of apoptosis and necrosis. Human Cell, 11: 3-12, 1998. 349. Juan, G., and Darzynkiewicz, Z.: Phosphorylation of pRb in individual HL-60 cells during their proliferation and differentiation. Exp. Cell Res., 244: 83-92, 1998. 350. Deptala, A., Bedner, E., Gorczyca, W., and Darzynkiewicz, Z.: Simple assay of activation of nuclear factor kappa B (NF-?B) by laser scanning cytometry (LSC). Cytometry, 33: 376-382, 1998. 351. Mikulski, S., Viera, A., Deptala, A., Shogen, K., and Darzynkiewicz, Z.: Anti-tumor activity synergism between onconase and proteasome inhibitors. Int. J. Oncol., 13: 633-644, 1998. 352. Feldman, E., Seiter, K., Chiao, J.W., Halicka, H.D., Traganos, F., Fatora, S.R., McMichael J., Baskind, P., Goff, H., Beer, M, Ahmed, T., and Darzynkiewicz, Z.: In vitro and clinical evaluation of a human chorionic gonadotrophin praparation in acute leukemia. Leukemia, 12, 1749-1755, 1998. 353. Juan, G., and Darzynkiewicz, Z.: Phosphorylation of retinoblastoma protein (pRb) assayed in individual cells by multilaser flow cytometry. Clinical Immunol. Newsletter, 18: 89-94, 1998. 354. Gorczyca W., Bedner, E., Burfeind, P., Darzynkiewicz, Z., and Melamed, M.R.: Analysis is apoptosis in solid tumors by laser scanning cytometry. Mod. Pathol., 11: 1-7, 1998. 355. Gorczyca, W., Melamed, M.R., and Darzynkiewicz, Z.: Analysis of apoptosis by flow cytometry. Methods Mol. Biol., 91: 217-38, 1998. 356. Gorczyca, W., Davidian M., Gherson, J., Ashikari R., Darzynkiewicz, Z., and Melamed, M.R.: Laser scanning cytometry quantification of estrogen receptors in breast cancer. Anal. Quant. Cytol. Histol., 20: 470-476, 1998. 1999 357. Del Bino, G., Darzynkiewicz, Z., Degraef, C., Mosselmans, R., and Galand, P.: Comparison of methods based on annexin V binding, DNA content or TUNEL for evaluating cell death in HL-60 and adherent MCF-7 cells. Cell Prolif., 32: 25-37, 1999. 358. Bedner E., Li, X., Gorczyca W., Melamed, M.R., and Darzynkiewicz, Z.: Analysis of apoptosis by laser scanning cytometry. Cytometry, 35: 181-195, 1999. 359. Tan, L., Kowalska, M.A., Romo, G., Lopez, G., Darzynkiewicz, Z., and Niewiarowski, S.: Identification and characterization of endothelial glycoprotein Ib using Viper venom proteins. Blood, 93: 2605-2616, 1999 360. Juan, G., Chang, W., Traganos, F and Darzynkiewicz, Z.: Histone H3 phosphorylation in human monocytes and during HL-60 cell differentiation. Exp. Cell Res., 246: 212-229, 1999. 361. Darzynkiewicz, Z.: EDITORIAL: The butler did it: Search for killer(s) of Kaposi's sarcoma cells in preparations of human chorionic gonadotropin. JNCI, 91: 104-106, 1999 362. Bedner E., Halicka, H. D., Cheng, W., Salomon, T., Deptala, A., Gorczyca, W., Melamed, M.R. and Darzynkiewicz, Z.: High affinity binding of fluorescein isothiocyanate to eosinophils detected by laser scanning cytometry. A potential source of error in analysis of blood samples utilizing fluorescein-conjugated reagents in flow cytometry. Cytometry, 36: 77-82, 1999. 363. Darzynkiewicz, Z., Bedner, E., Gorczyca, W., and Melamed, M.R.: Laser scanning cytometry. A new instrumentation with many applications. Mini-Review. Exp. Cell Res., 249: 1-12, 1999. 364. Hsieh, T.-C., Juan, G., Darzynkiewicz, Z and Wu, J.M.: Resveratrol increases nitric oxide synthase, induces accumulation of p53 and p21WAF1/CIP1 and suppresses cultured bovine pulmonary artery endothelial cell proliferation by perturbing progression through S and G2. Cancer Res., 59: 2596-2601, 1999. 365. Li, X., and Darzynkiewicz, Z.:The Schrödinger's cat quandary in cell biology: Integration of live cell functional assays with measurements of fixed cells in analysis of apoptosis. Exp. Cell Res., 249: 404-412, 1999. 366. Hsieh, T-C., Burfeind, P., Laud, K., Backer, J.M., Traganos, F., Darzynkiewicz, Z. and Wu, J.M: Cell cycle effects and control of gene expression by resveratrol in breast carcinoma cell lines with different metastatic potential. Int. J. Oncol., 15: 245-252, 1999. 367. Deptala, A., Li,X., Bedner, E., Cheng, W., Traganos, F., and Darzynkiewicz, Z.: Differences in induction of p53, p21WAF1/CIP1 and apoptosis in relation to cell cycle phase of MCF-7 cells treated with camptothecin. Int. J. Oncol., 15: 861-871, 1999. 368. Deptala, A., Halicka, H.D., Salomon, T., Gorczyca, W., Seiter, W., Feldman, E.J., Traganos, F., and Darzynkiewicz, Z. Malignancy: a new approach to the analysis of apoptosis in the leukemia subpopulations by flow cytometry using a CD45 gating strategy. Hematol. 4: 113-121, 1999. 2000 369. Seiter, K., Feldman, E.J., Halicka, H.D., Deptala, A., Traganos, F., Burke, H.B., Hoang, A., Goff, H., Pozuollo, M., Kancherla, R., Darzynkiewicz, Z., and Ahmed, T.: Clinical and laboratory evaluation of all-trans retinoic acid modulation of chemotherapy in patients with acute myelogenous leukemia. Br. J. Hematol., 108: 40-47, 2000 370. Li, X., and Darzynkiewicz, Z.: Cleavage of poly(ADP-ribose) polymerase measured in situ in individual cells: relationship to DNA fragmentation and cell cycle position during apoptosis. Exp. Cell Res., 255: 125-132, 2000. 371. Kajstura, J., Pertoldi, B., Leri, A., Beltrami, C-A., Deptala, A., Darzynkiewicz, Z. and Anversa, P.: Telomere shortening: an in vivo marker of myocyte replication and aging. Am. J. Pathol., 156: 813-819, 2000. 372. Lin, C-W., Darzynkiewicz, Z., Li, X., Traganos, F.,Bedner, E., and Tse-dinh, Y-C., Nucleolar localization and cell cycle dependent cleavage of human topoisomerase III" in PHA activated peripheral blood lymphocytes. Exp. Cell Res., 256: 225-236, 2000. 373. Feldman, E.J., Seiter, K., Traganos, F., Darzynkiewicz, Z., Goff, H., Pozzuoli, M., Baskind, P., Santos, S., and Ahmed T., Phase II evaluation of a high-dose mitoxantrone based induction regimen in untreated adults with acute myeloid leukemia. Leuk. Lymphoma, 38:309-15, 2000. 374. Darzynkiewicz, Z. Re: Preclinical and clinical development of cyclin-dependent kinase modulators. Correspondence letter. JNCI, 92: 1184-1185, 2000 375. Halicka, H. D., Murakami, T., Papageorgio,C.N., Mittelman, A., Mikulski, S.M., Shogen, K., and Darzynkiewicz, Z.: Induction of differentiation of leukemic (HL-60) or prostate cancer (LNCaP, JCA-1) cells potentiates frequency of apoptosis triggered by onconase. Cell Prolif., 33: 407-417, 2000. 376. Bedner, E., Ruan,Q. Chen, S., Kamentsky, L.A., and Darzynkiewicz, Z. Multiparameter analysis of progeny of individual cells by laser scanning cytometry. Cytometry, 40: 271-279, 2000. 377. Li, X., Du, L., and Darzynkiewicz, Z.: Caspases are activated during apoptosis independently of dissipation of mitochondrial electrochemical potential. Exp Cell Res., 257: 290-297, 2000. 378. Albino, A.P., Juan, G., Traganos, F., Reinart, L., Connolly, J., Rose, D.P., and Darzynkiewicz, Z.: G1 arrest of melanoma cells by docosahexaenoic acid (omega-3 fatty acid) is associated with decreased pRB phosphorylation. Cancer Res., 60: 4130-4145, 2000. 379. DiPietranonio, A.M., Hsieh, T.-C., Juan, G.,Traganos , F., Darzynkiewicz, Z., and Wu, J. Fenretinide-induced caspase-3 activity involves increased protein stability in a mechanism distinct from reactive oxygen species elevation. Cancer Res., 60: 4331-4335, 2000. 380. Darzynkiewicz, Z., Traganos, F., and Chen, S.: Chinese herbal mixture PC-SPES in treatment of prostate cancer. A review. Int. J. Oncol., 17: 729-736, 2000. 381. Bedner, E., Li, X., Kunicki, J., and Darzynkiewicz, Z. Translocation of Bax to mitochondria during apoptosis measured by laser scanning cytometry. Cytometry, 41: 83-88, 2000. 382. Bedner, E., Smolewski, P., Amstad, P., and Darzynkiewicz, Z. Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp. Cell Res., 259: 308-313, 2000. 383. Abdel-Moneim, I., Melamed, M.R., Darzynkiewicz, Z., and Gorczyca, W.: Proliferation and apoptosis in solid tumors. Analysis by laser scanning cytometry. Analyt. Quant. Cytol. Histol., 22: 393-397, 2000. 384. Halicka, H. D., Bedner, E., and Darzynkiewicz, Z. Segregation of RNA and separate packaging of DNA and RNA in apoptotic bodies during apoptosis. Exp. Cell. Res., 260: 248-255, 2000. 385. Weisberger, J., Wu, C.D., Liu, Z., Wong J.Y.I., Melamed, M.R., Darzynkiewicz, Z., and Gorczyca, W. Differential diagnosis of malignant lymphomas and related disorders by specific pattern of expression of immunophenotypic markers revealed by multiparameter flow cytometry (Review). Int. J. Oncol., 17: 1165-1177, 2000. 386. Zhang, J.C., Claffey, K., Sakthivel, R., Darzynkiewicz, Z., Shaw, D.E., Leal, J, Wang, Y.C., Lu, F.M., and MacCrae, K.R. Two-chain high molecular weight kininogen induces endothelial cell apoptosis and inhibits angiogenesis: partial activity with domain 5. FASEB J,. 14: 2589-2600, 2000. 2001 387. Chen, S., Ruan, Q., Deptala, A., Hsieh, T.-C., Wang, X., Traganos, F., and Darzynkiewicz, Z.,: Effects of herbal preparation PC-SPES and its active flavonoid component baicalin on androgen receptor, cell cycle and apoptosis of prostate cancer lines. Cell Prolif. 34: 293-304, 2001. 388. Dobrucki J., and Darzynkiewicz, Z. Chromatin condensation and sensitivity of DNA in situ to denaturation during cell cycle and apoptosis. A confocal microscopy study. Micron, 32: 645-652, 2001 389. Bedner, E., Du, L., Traganos, F., and Darzynkiewicz, Z. Caffeine dissociates complexes between DNA and intercalating dyes. Application for bleaching fluorochrome-stained cells for their subsequent re-staining and analysis by laser scanning cytometry. Cytometry, 43: 38-45, 2001. 390. Ardelt, B., Kunicki, J., Traganos, F., and Darzynkiewicz, Z. Chlorophyllin protects cells from the cytostatic and cytotoxic effects of quinacrine mustard but not of nitrogen mustard. Int. J. Oncol., 18: 849-853, 2001. 391. Darzynkiewicz Z., Bedner, E., and Smolewski, P. Flow cytometry in analysis of cell cycle and apoptosis. Semin. Hematol., 38: 179-193, 2001. 392. Smolewski, P., Bedner, E., Du, L., Hsieh, T-C., Wu, J.M., Phelps, D.J. and Darzynkiewicz, Z. Detection of caspases activation by fluorochrome-labeled inhibitors: multiparameter analysis by laser scanning cytometry, Cytometry, 44: 73-82, 2001. 393. Deptala, A., Bedner, E., and Darzynkiewicz, Z. Unique analytical capabilities of laser scanning cytometry that complement flow cytometry. Folia Histochem. Cytobiol., 39: 87-89, 2001. 394. Darzynkiewicz, Z., Bedner, E., and Smolewski, P. New approaches in analysis of apoptosis by cytometry. Acta Haematol. Polon. 32 (supplement 1), 77-79, 2001. 395. Darzynkiewicz, Z., Li, X., and Bedner, E. Use of flow and laser scanning cytometry in analysis of cell death. Meth Cell Biol. 66: 69-109, 2001. 396. Smolewski, P., Bedner, E., Gorczyca, W., and Darzynkiewicz, Z. "Liquidless" cell staining by dye diffusion from gels and analysis by laser scanning cytometry: potential application at microgravity conditions in space. Cytometry, 44: 355-360. 2001. 397. Leri, A., Berlucchi I., Limana, F., Deptala, A., Darzynkiewicz, Z., Hintze, T.H., Kajstura, J., Nadal-Ginard, B., Anversa, P. Telomerase expression and activity are coupled with myocyte proliferation and preservation of telomeric length in the failing heart. Proc. Natl. Acad. Sci. USA. 98: 8626-31; 2001. 398. Smolewski, P., Grabarek, J., Kamentsky, L.A., and Darzynkiewicz, Z. Bivariate analysis of cellular DNA vs RNA content by laser scanning cytometry using the product of signal subtraction (Differential Fluorescence; DF) as a separate parameter. Cytometry, 45: 73-78, 2001. 399. Smolewski, P., Ruan, Q Vellon, L., and Darzynkiewicz, Z. The micronuclei assay by laser scanning cytometry. Cytometry, 45: 19-26, 2001. 400. Smolewski, P., Grabarek, J., Phelps, D.J. and Darzynkiewicz, Z. Stathmo-apoptosis: arresting apoptosis by fluorochrome-labeled inhibitor of caspases. Int. J. Oncol. 19: 657-663, 2001. 401. Darzynkiewicz, Z., Smolewski, P., and Bedner, E. Use of flow- and laser scanning- cytometry to study mechanisms regulating cell cycle and controlling cell death. Clinics Lab. Med. 21: 857-873, 2001. 402. Gorczyca, W., Smolewski, P., Grabarek, J., Ardelt, B., Ita, M., Melamed, M.R., and Darzynkiewicz, Z. Morphometry of nucleoli and expression of nucleolin analyzed by laser scanning cytometry in mitogenically stimulated lymphocytes. Cytometry, 45: 206-213, 2001. 403. Du, L., Smolewski, P., Bedner, E., Traganos, F., and Darzynkiewicz, Z. Selective protection of mitogenically stimulated human lymphocytes but not leukemic cells from cytosine arabinoside-induced apoptosis by LY294002, a phosphoinositol-3 kinase inhibitor. Int. J. Oncol ., 19: 811-820, 2001. 2002 404. Moog-Lutz, C., Guibal, F.C., Cave-Riant, F., Smolewski, P., Di Giois Y., Darzynkiewicz, Z., Lutz, P.G. and Cayre, Y.E. ASB-2 inhibits growth and promotes commitment in myeloid leukemia cells J. Biol. Chem., 277: 218-224, 2002. 405. Darzynkiewicz, Z. To cycle or not to cycle ? The role of checkpoints in maintenance of genome integrity and cancer. Cancer Biology & Therapy, 1: 76-77, 2002. 406. Grabarek, J., Dragan, M., Lee, B.W., Johnson, G.L. and Darzynkiewicz, Z. Activation of chymotrypsin-like serine protease(s) during apoptosis detected by affinity-labeling of the enzymatic center with the fluoresceinated inhibitor. Int. J. Oncol., 20: 225-233, 2002. 407. Smolewski, P., Grabarek, J., Lee, B.W., Johnson, G.L. and Darzynkiewicz, Z. Kinetics of HL-60 cell entry to apoptosis during treatment with TNF-a or camptothecin assayed by the stathmo-apoptosis method. Cytometry, 47: 143-149, 2002 408. Darzynkiewicz, Z., Smolewski, P., and Bedner, E. Use of flow and laser scanning cytometry to study mechanisms regulating cell cycle and controlling cell death. Hematology/Oncology Clinics of North America, 16: 1-17, 2002. 409. Hsieh, T.C., Lu, X., Guo, J., Xiong, W, Kunicki, J., Darzynkiewicz, Z. and Wu, J.M. Effects of herbal preparation Equiguard on hormone-responsive and hormone-refractive prostate carcinoma cells: mechanistic studies. Int. J. Oncol., 20: 681-689, 2002. 410. Tsai , S.Y., Hsieh, T.C., Ardelt, B., Darzynkiewicz, Z., and Wu, J.M. Combined effects of onconase and IFN-beta on proliferation, macromolecular synthesis and expression of STAT- in JCA-1 cells. Int. J. Oncol., 20: 891-896, 2002. 411. Kapuscinski, J., Ardelt, B., Piosik, J., Zdunek, M., Darzynkiewicz, Z. The modulation of the DNA-damaging effect of polycyclic aromatic agents by xanthines. Part I. Reduction of cytostatic effects of quinacrine mustard by caffeine. Biochem. Pharmacol., 63: 625-634, 2002. 412. Wang. Q., Xie, S., Chen, J., Fukasawa, K, Naik, U., Traganos, F., Darzynkiewicz, Z., Jhanwar-Uniyal, M., Dai, W. Cell cycle arrest and apoptosis induced by human polo-like kinase 3 is mediated through perturbation of microtubule integrity. Molec. Cell Biol., 22: 3450-3459, 2002. 413. Grabarek, J., and Darzynkiewicz, Z.: Versatility of analytical capabilities of laser scanning cytometry. Clin. Appl. Immunol. Rev. (CAIR) 2: 75-92, 2002. 414. Darzynkiewicz, Z., Bedner, E., and Smolewski, P. In situ detection of DNA strand breaks in analysis of apoptosis by flow- and laser scanning- cytometry. Meth. Molec. Biol. 203: 69-77, 2002. 415. Darzynkiewicz, Z., Bedner, E., Smolewski, P., Lee, B.W., and Johnson, G.L. Detection of caspases activation in situ by fluorochrome-labeled inhibitors of caspases (FLICA). Meth. Molec. Biol. 203: 289-299, 2002. 416. Tsai, S.Y, Hsieh, T.C., Ardelt, B., Darzynkiewicz, Z., and Wu, J.M. Combined effects of onconase and INF-beta on proliferation, macromolecular syntheses and expression of STAT-1 in JCA-1 cancer cells. Int. J. Oncol., 20: 891-896, 2002. 417. Smolewski, P., Grabarek, J., Halicka, H.D., and Darzynkiewicz, Z. Assay of caspases activation in situ combined with probing plasma membrane integrity detects three distinct stages of apoptosis. J. Immunol. Meth., 265: 111-121, 2002. 418. Gorczyca, W., Weisberger, J., Liu, Z., Tsang, P., Hossein, M., Wu, C.D., Dong, H., Wong, J. Y.L., Tugulea, S., Dee, S., Melamed, M.R., and Darzynkiewicz, Z. An approach to diagnosis of T-cell lympho-proliferative disorders by flow cytometry. Cytometry (Clinical Cytometry) 50: 177-190, 2002. 419. Darzynkiewicz, Z., Bedner, E., and Smolewski, P. Analysis of apoptosis by laser scanning cytometry. Meth. Pharmacol. Toxicol., Apoptosis Methods in Pharmacology and Toxicology. M.A/. Davis Ed., Humana Press (Totowa, NJ) 37-58, 2002. 420. Grabarek, J., Du. L., Johnson, G.L., Lee, B., Phelps, D.J., and Darzynkiewicz, Z.: Sequential activation caspases and serine proteases (serpases) during apoptosis. Cell Cycle, 1: 124-131, 2002. 421. Grabarek, J., Amstad, P., and Darzynkiewicz, Z. Use of fluorescently labeled inhibitors of caspases as affinity labels to detect activated caspases. Human Cell. 15: 1-12, 2002. 422. Darzynkiewicz, Z., Smolewski, P., and Bedner, E. Use of flow and laser scanning cytometry to study mechanisms regulating cell cycle and controlling cell death. Hematol/Oncol Clinics of North America. 16: 339-355, 2002 423. Grabarek, J., Ardelt, B., Du, L., and Darzynkiewicz, Z. Activation of caspases and serine proteases during apoptosis induced by onconase (Ranpirnase). Exp. Cell Res., 278: 61-71, 2002. 424. Smolewski, P., Grabarek, J., Halicka, H.D., and Darzynkiewicz, Z. Assay of caspases activation in situ combined with probing plasma membrane integrity detects three distinct stages of apoptosis. J. Immunol. Meth., 265: 111-121, 2002. 425. Hsieh, T-C., Xiong, W, Traganos, F., Darzynkiewicz, Z., and Wu, J.M. Effects of PC-SPES on proliferation and expression of AR/PSA in androgen responsive LNCaP cells are independent of estradiol. Anticancer Res., 22: 2051-2060, 2002. 426. Grabarek. J., and Darzynkiewicz, Z. In situ activation of caspases and serine proteases during apoptosis detected by affinity labeling their enzyme active centers with fluorochrome-tagged inhibitors. Exp. Hematol. 30: 982-992, 2002. 427. Halicka, H.D., Smolewski, P., Darzynkiewicz, Z., Dai, W., and Traganos, F. Arsenic trioxide arrests cells early in mitosis leading to apoptosis. Cell Cycle, 1: 201-209, 2002. 429. Blagosklonny, M.V., Darzynkiewicz, Z., and Figg, W.D. Flavopiridol inversely affects p21WAF1/CIP1 and p53 and protects p21-sensitive cells from paclitaxel. Canc. Biol. Ther. 1: 424-430, 2002. 430. Xie, S., Wang, Q., Luo, L., Ruan, Q., Liu, T., Jhanhwar-Uniyal, M., Darzynkiewicz, Z., Traganos, F., and Dai, W. Proteasome-dependent down-regulation of p21WAF1/CIP1 induced by reactive oxygen species. J. Interferon & Cytokine Res. 22: 957-963, 2002. 431. Hsieh, T-C., Halicka, D., Lu, X., Kunicki, J., Guo, J., Darzynkiewicz, Z., and Wu, J.M. Effects of resveratrol on the G0 to G1 transition and cell cycle progression of mitogenically stimulated human lymphocytes. Biochem. Biophys. Res. Commun. 297: 1311-1317, 2002. 432. Teodori, L., Grabarek, J., Smolewski, P., Ghibelli, L., Bergamaschi, A., De Nicola, M., and Darzynkiewicz, Z., Exposure of cells to static magnetic field accelerates loss of integrity of plasma membrane during apoptosis. Cytometry, 49: 113-119, 2002. 433. Grabarek, J., Ardelt, B., Kunicki, J., and Darzynkiewicz, Z. Detection of in situ activation of transglutaminase during apoptosis: correlation with the cell cycle phase by multiparameter flow- and laser scanning- cytometry. Cytometry, 49: 83-89, 2002. 434. Blagosklonny, M.V and Darzynkiewicz, Z. Four birds with one stone: RAPA as potential anticancer therapy. Canc. Biol. Ther. 1: 363-365, 2002. 435. Blagosklonny, M.V., Robey, R., Sackett, D.L., Du, L., Traganos, F., Darzynkiewicz, Z., Fojo, T., and Bates, S.E. Histone deacetylase inhibitors all induce p21 but differentialy cause tubulin acetylation, mitotic arrest, and cytotoxicity. Molec. Canc. Ther., 1: 937-941, 2002. 436. Halicka D. H., Pozarowski, P., Ita, M., Ardelt, W., Mikulski, S.M., Shogen, K., and Darzynkiewicz, Z. Enhancement of activation-induced apoptosis of lymphocytes by the cytotoxic ribonuclease onconase (Ranpirnase). Int. J. Oncol., 21:1245-1250, 2002. 437. Hsieh, T.-C., Kunicki, J., Darzynkiewicz, Z., and Wu, M.J.: Effects of extract of Coloris versicolor (I'm-YunityTM ) on cell cycle progression and expression of interleukins 1-ß, -6, and -8 in promyelocytic HL-60 leukemic cells and mitogenically stimulated human lymphocytes. J. Alternative and Complementary Medicine, 8: 591-602, 2002. 438. Darzynkiewicz, Z. One more reason to get arrested (in G1). Cell Cycle, 1: 318-319, 2002. 439. Blagosklonny, M.V., and Darzynkiewicz, Z. Cyclotherapy: Protecting of normal cells and unshielding of cancer cells. Cell Cycle, 1: 375-382, 2002. 2003 440. Strife, A., Wisniewski, D., Chongyuan, L., Lambek, C.L., Darzynkiewicz, Z., Silver, R.T., and Clarkson, B. Direct evidence that Bcr-Abl tyrosine kinase activity disrupts normal synergistic interactions between Kit ligand and cytokines in primary primitive progenitor cells. Molec. Canc. Res., 1: 176-185, 2003. 441. Haider, A.S., Grabarek, J., Eng, B., Pedraza, P., Ferreri, N.R., Balazs, E.A., and Darzynkiewicz, Z. In vitro wound healing analyzed by laser scanning cytometry. Accelerated healing of epithelial cell monolayers in the presence of hyaluronate. Cytometry, 53A:1-8, 2003. 442. Ardelt, B., Ardelt, W., and Darzynkiewicz, Z. Cytotoxic ribonucleases and RNA interference (RNAi). Cell Cycle, 2: 22-24, 2003. 443. Smolewski, P., Darzynkiewicz, Z., and Robak, T. Caspase-mediated cell death in hematological malignancies: Theoretical considerations, methods of assessment, and clinical implications. Leukemia & Lymphoma, 44; 1089-1104, 2003. 444. Blagosklonny, M.V., and Darzynkiewicz, Z. Why Iressa failed: toward novel use of kinase inhibitors (outlook). Canc. Biol. Ther. 2: 137-140, 2003. 445. Pozarowski, P., Halicka D.H., and Darzynkiewicz, Z. NF-?B inhibitor sesquiterpene parthenolide induces concurrently atypical apoptosis and necrosis: Difficulties in identification of dead cells in such cultures. Cytometry, 54A: 118-124, 2003. 446. Smolewski, P., and Darzynkiewicz, Z. [Current methods for measurement apoptosis] (in Polish). Acta Haematol. Polon., 34: 35-47, 2003. 447. Pozarowski, P., Halicka, D.H. and Darzynkiewicz, Z. Cell cycle effects and caspase-dependent and independent death of HL-60 and Jurkat cells treated with the NF-?B inhibitor parthenolide. Cell Cycle, 2: 377-383, 2003. 448. Pietrzak M., Wieczorek, Z., Stachelska, A., and Darzynkiewicz, Z. Interactions of chlorophyllin with acridine orange, quinacrine mustard and doxorubicin analyzed by light absorption and fluorescence spectroscopy. Biophys. Chem., 104; 305-313, 2003. 449. Aoe, K., Hiraki, A, Murakami, T., Murakami, K., Makihata, K., Takao, K., Eda, R., Maeda, T., Sugi, K., Darzynkiewicz, Z., and Takeyama, H. Relative abundance and patterns of correlation among six cytokines in pleural fluid measured by cytometric bead array. Int. J. Molec. Med. 12: 193-198, 2003. 450. Pozarowski P, Huang, X., Halicka D.H., Lee, B., Johnson, G., and Darzynkiewicz Z. Interactions of fluorochrome-labeled caspase inhibitors with apoptotic cells. A caution in data interpretation. Cytometry 55A: 50-60, 2003. 451. Darzynkiewicz, Z., Juan, G., and Traganos, F. Cytometry of cell cycle regulatory proteins. Prog. Cell Cycle Res., 5: 533-542, 2003. 452. Lee, B.W., Johnson, G.L., Hed, S.A., Darzynkiewicz, Z., Talhouk, J.W., Mehrota, S. DEVDase detection in intact apoptotic cells using the cell permeant fluorogenic substrate, (z-DEVD)2-cresyl violet. Biotechniques, 35:1080-1085, 2003. 453. Huang X, Traganos F, Darzynkiewicz Z. DNA damage induced by DNA topoisomerase I- and topoisomerase II- inhibitors detected by histone H2AX phosphorylation in relation to the cell cycle phase and apoptosis. Cell Cycle, 2: 614-619; 2003. 454. Abraham, N.G., Kushida, T., McClung, J., Quan, S., Lafaro, R., Darzynkiewicz, Z., and Wolin, M. Heme oxygenase-1 attenuates glucose-mediated cell growth arrest and apoptosis in human microvessel endothelial cells. Circulation Research, 93: 507-514, 2003. 455. Haider, A., Lee, I., Grabarek, J., Darzynkiewicz, Z., and Ferreri, N.R. Dual functionality of cyclooxygenase-2 as a regulator of tumor necrosis factor-mediated G1 shortening and nitric oxide-mediated inhibition of vascular smooth muscle cell proliferation. Circulation, 108: 1015-1021, 2003. 2004 456. Pozarowski, P., Huang, X., Gong, R.W., Priebe, W., Darzynkiewicz, Z. Simple, semi-automatic assay of cytostatic and cytotoxic effects of antitumor drugs by laser-scanning cytometry (LSC): Effects of the bis-intercalator WP631 on growth and cell cycle of T-24 cells. Cytometry, 57A: 113-119, 2004 454. Pozarowski, P., Huang, X., Gong, R.W., Priebe, W., Darzynkiewicz, Z. Simple, semi-automatic assay of cytostatic and cytotoxic effects of antitumor drugs by laser-scanning cytometry (LSC): Effects of the bis-intercalator WP631 on growth and cell cycle of T-24 cells. Cytometry A, 57A: 113-119, 2004 455. Wang, Q., Tongyi Liu, T ., Fang, Y., Xie, S., Huang, X., Mahmood, R., Ramasiwamy, M., Sakamoto, K.M., Darzynkiewicz, Z., Xu, M., Dai, W. BUBR-1 deficiency results in abnormal megakaryopoiesis. Blood, 103: 1278-1285, 2004. 456. Smolewska, E., Brozik, H., Smolewski, P., Darzynkiewicz, Z., and Stanczyk, J. Regulation of peripheral blood and synovial fluid lymphocyte apoptosis in juvenile idiopathic arthritis. Scand. J. Rheumatol. 33: 1-6. 2004. 457. Ruan Q, Wang, Q., Xie, S., Fang, Y., Darzynkiewicz, Z., Guan, K., Jhanwar-Uniyal, M., Dai, W. Polo-like kinase 3 is Golgi localized and involved in regulating Golgi fragmentation during the cell cycle. Exp. Cell Res., 294: 51-59, 2004. 458. Darzynkiewicz Z., Crissman, H.A., and Jacobberger, J.W. Cytometry of the cell cycle. Cycling through history. Cytometry, 58A; 21-32, 2004. 459. Huang, X., Okafuji, M., Traganos, F., Luther, E., Holden, E., Darzynkiewicz, Z. Assessment of histone H2AX phosphorylation induced by DNA topoisomerase I and II inhibitors topotecan and mitoxantrone and by DNA crosslinking agent cisplatin. Cytometry A, 58A: 99-110, 2004. 460. Huang, X., Ruan, Q., Fang, Y., Traganos, F., Darzynkiewicz, Z., and Dai, W. Physical and functional interactions between mitotic kinases during polyploidization and megakaryocytic differentiation. Cell Cycle 3: 946-951, 2004 461. Albino A.P., Huang, X., Yang, J., Gietl,D. Jorgensen, E., Traganos, F., and Darzynkiewicz, Z. Induction of histone H2AX phosphorylation in A549 human pulmonary epithelial cells by tobacco smoke and in human bronchial epithelial cells by smoke condensate: A new assay to detect the presence of potential carcinogens in tobacco. Cell Cycle, 3: 1062-1068, 2004. 462. Blagosklonny, V.M., Darzynkiewicz, Z., Halicka, H. D., Pozarowski, P., Demidenko, Z., Barry, J.J., Kamath, K.R., and Herrman, R.A. Paclitaxel induces primary and postmitotic G1 arrest in human arterial smooth muscle cells. Cell Cycle, 3: 1050-1056, 2004. 463. Jamroziak, K., Smolewski, P., Cebula, B., Szmigielska-Kaplon, A., Darzynkiewicz, Z., and Robak, T. Relation of P-glycoprotein expression with spontaneous in vitro apoptosis in B-cell chronic lymphocytic leukemia. Neoplasma, 51: 181-187, 2004. 464. Fu, C, Hsieh, T-T., Guo, J., Kunicki, J., Lee, M.Y.W.T., Darzynkiewicz, Z., and Wu, J.M. Licochalcone-A, a novel flavonoid, causes G2 and late-G1 arrests in PC-3 cells. Biochem. Biphys. Res. Commun. 322: 263-270, 2004. 465. Hiraki, A., Fuji, N., Murakami, T., Kiura, K., Aoe, K., Yamane, H., Masuda, K., Maeda, T., Sugi, K., Darzynkiewicz, Z., Tanimoto, M., and Harada, M. High frequency of allele-specific down-regulation of HLA class I expression in lung cancer lines. Anticancer Res., 24: 1525-1528, 2004. 466. Huang, X., King, M., Halicka, D.H., Traganos, F., Okafuji, M., and Darzynkiewicz, Z. Histone H2AX phosphorylation induced by selective photolysis of BrdU-labeled DNA with UV light: relationship to cell cycle phase. Cytometry A, 62A: 1-7, 2004 467. King M.A., Halicka, H.D., and Darzynkiewicz, Z. Pro- and anti- apoptotic effects of an inhibitor of chymotrypsin-like serine proteases. Cell Cycle 3: 1666-1571, 2004 468. Tsai, S.Y., Ardelt, B., Hsieh, T-C., Darzynkiewicz, Z., and Wu, J.M. Treatment of Jurkat acute T-lymphocytic leukemia cells by onconase is accompanied by an altered nucleocytoplasmic distribution and reduced expression of transcription factor NF- B. Int. J. Oncol., 25: 1745-1752, 2004. 2005 469. Chen, S., Gao, J., Halicka, H.D., Huang, X., Traganos, F., and Darzynkiewicz, Z. The cytostatic and cytotoxic effects of oridonin (Rubescenin), a diterpenoid from Rabdosia Rubescens on tumor cells of different lineage. Int. J. Oncol. , 26: 579-588. 2005. 470. Gupte, S. R., Pozarowski, P., Grabarek, J., Traganos, F., Darzynkiewicz, Z., and Lee, M.Y.W.T. RI? influences cell proliferation in cancer cells by transporting RFC40 into the nucleus. Cancer Biol. Ther. 4: 429-437, 2005. 471. Demidenko, Z.N., Halicka D., Kunicki, J., McCubrey, J.A., Darzynkiewicz, Z., and Blagosklonny, M.V. Selective killing of adriamycin-resistant (G2 Checkpoint-deficient and MRP-1-expressing) cancer cells by docetaxel. Cancer Res., 65: 4401-4407, 2005. 472. Pozarowski, P., Holden, E., and Darzynkiewicz, Z.: Laser scanning cytometry. Principles and applications. Meth. Molec. Biol. 319: 165-192, 2005 473. Huang X, Lee, B., Johnson, G., Naleway, J., Guzikowski, A., Dai, W., and Darzynkiewicz, Z. Novel assay utilizing fluorochrome-tagged physostigmine (Ph-F) to in situ detect active acetylcholinesterase (AChE) induced during apoptosis. Cell Cycle, 4: 140-147; 2005 474. Huang, H.S., Pozarowski, P., Gao, Y., Darzynkiewicz, Z., Lee, E.Y. Protein phosphatase-1 inhibitor-3 is co-localized to the nucleoli and centrosomes with PP1gamma1 and PP1alpha, respectively. Arch Biochem Biophys. 443: 33-44, 2005. 475. Halicka, H.D., Huang, X., Traganos F., King, M.A., Dai, W., and Darzynkiewicz, Z. Histone H2AX phosphorylation after cell irradiation with UV-B: Relationship to cell cycle phase and induction of apoptosis. Cell Cycle, 4: 339-345, 2005. 476. Friedrich T.D., Bedner, E., Darzynkiewicz, Z., and Lehman, J.M. Rereplication of host cell DNA without reloading MCM proteins in Simian virus 40 lytic infection. Cytometry A, 68A: 10-18, 2005. 477. Huang, X., Dai, W., and Darzynkiewicz, Z. Enforced adhesion of hematopoietic cells to culture dish induces endomitosis and polyploidy. Cell Cycle, 4: 801-505, 2005 478. Huang, X., Halicka, H.D., Traganos, F., Tanaka, T., Kurose, A., and Darzynkiewicz, Z. Cytometric assessment of DNA damage in relation to cell cycle phase and apoptosis. Cell Proliferation, 38: 223-243, 2005. 479. Kurose, A.,Tanaka, T., Huang X., Halicka, H.D., Traganos F., Dai, W., and Darzynkiewicz, Z. Assessment of ATM phosphorylation on Ser-1981 induced by DNA topoisomerase I and II inhibitors in relation to Ser-139-histone H2AX phosphorylation, cell cycle phase and apoptosis. Cytometry A, 68 A: 1-9, 2005. 480. Jain, K., Roy, U., Ardelt, B., Krishna, UM., Falck, J.R., Pozarowski, P., Kunicki, J., Darzynkiewicz, Z., and Balazy, M. 5E, 8Z, 14Z-eicosatetraenoic acid, a novel trans isomer of arachidonic acid, causes G1 phase arrest and induces apoptosis of HL-60 cells. Int. J. Oncol., 27: 1177-1185, 2005. 2006 481. Chen, J., Huang, X., Halicka, D., Brodsky, S., Avram, A., Eskander, J., Bloomgarden, N.A., Darzynkiewicz, Z., Goligorsky, M.S. Contribution of p16INK4a and p21CIP1 pathways to induction of premature senescence of human endothelial cells: Permissive role of p53. Am. J. Physiol. Heart. Circ. Physiol., 290: H1575-1586, 2006 482. Tanaka, T., Kurose, A., Huang, X., Dai, W., and Darzynkiewicz, Z. ATM kinase activation and histone H2AX phosphorylation as indicators of DNA damage by DNA topoisomerase I inhibitor topotecan and during apoptosis. Cell Proliferation, 39: 49-60, 2006. 483. Kurose, A., Tanaka, T., Huang, X., Traganos, F., Dai, W., Darzynkiewicz, Z. Effects of hydroxyurea and aphidicolin on phosphorylation of ATM on Ser 1981 and histone H2AX on Ser 139 in relation to cell cycle phase and induction of apoptosis. Cytometry A, 69A: 212-221, 2006. 484. Albino, A.P., Huang, X., Jorgensen, E., Gietl, D., Traganos, F. and Darzynkiewicz, Z. Induction of DNA double-strand breaks in A549 and normal human pulmonary epithelial cells by cigarette smoke is mediated by free radicals. Int J. Oncol. 28: 1491-1505, 2006. 485. Gupte R. S., Sampson, V., Traganos, F., Darzynkiewicz, Z and Lee M. Y. W. T. Cyclic AMP regulates the expression and nuclear translocation of RFC40 in MCF7 Cells. Exp Cell Res., 212: 976-806, 2006. 486. Fang, Y., Li, T., Wang, X., Yang, Y-M., Deng, H., Kunicki, J., Traganos, F., Darzynkiewicz, Z., Lu, L., and Dai, W. BubR1 is involved in regulation of DNA damage responses. Oncogene, 25: 3598-3605, 2006. 487. Huang, X., Kurose A., Tanaka, T., Traganos, F., Dai, W., and Darzynkiewicz, Z. Sequential phosphorylation of Ser-10 on histone H3 and Ser-139 on histone H2AX and ATM activation during premature chromosome condensation: Relationship to cell-cycle and apoptosis. Cytometry A, 69A: 222-229, 2006. 488. Fuji N, Hiraki, A., Aoe, K., Murakami, T., Ikeda, K., Masuda, K., Matsuo, K., Shinagawa, K., Ishimaru, F., Sugi, K., Darzynkiewicz, Z., and Tanimoto, M. Serum cytokine concentrations and acute graft-versus-host disease after allogeneic peripheral blood stems cell tranmsplantation: Concurrent measurement of ten cytokines and their respective ratios using cytometric bead assay. Int. J. Mol. Med., 17: 881-885, 2006. 489. Gupte, R.S., Traganos, F., Darzynkiewicz, Z., Lee, Y.W.T. Phosphorylation of RI by cyclin- dependent kinase CDK 2/Cyclin E modulates the dissociation of the RI -RFC40 complex. Cell Cycle, 5: 653-660, 2006. 490. Pietrzak, M., Wieczorek, Z., Wieczorek, J., and Darzynkiewicz, Z. The "interceptor" properties of chlorophyllin measured spectroscopically within the three-component system: intercalator- DNA-chlorophyllin. Biophys. J. 123: 11-19, 2006. 491. Huang, X., Tanaka, T., Kurose, A., Traganos, F., Darzynkiewicz, Z. Constitutive histone H2AX phosphorylation on Ser-139 in cells untreated by genotoxic agents is cell-cycle phase specific and attenuated by scavenging reactive oxygen species. Int. J. Oncol. 29: 495-501, 2006 492. Kurose, A., Tanaka, T., Huang, X., Traganos, F., Darzynkiewicz, Z. Synchronization in the cell cycle by inhibitors of DNA replication induces histone H2AX phosphorylation, an indication of DNA damage. Cell Proliferation 39: 231-240, 2006. 493. Jiang, N., Wang, X, Jhanwar-Uniyal, M., Darzynkiewicz, Z., and Dai, W. Polo-box domain of PLK3 functions as a centrosome localization signal, over-expression of which causes mitotic arrest, cytokinesis defects, and apoptosis. J. Biol. Chem. 281: 10577-82, 2006. 494. Tanaka, T., Kurose, A., Halicka, H.D., Traganos, F., and Darzynkiewicz, Z. 2-Deoxy-D-glucose reduces the level of constitutive activation of ATM and phosphorylation of histone H2AX. Cell Cycle, 5: 878-882, 2006. 495. Tanaka, T., Kurose, A., Huang, X., Traganos, F., Dai, W. and Darzynkiewicz. Extent of constitutive histone H2AX phosphorylation on Ser-139 varies in cells with different TP53 status. Cell Proliferation 39: 313-323, 2006. 496. Huang, X., Kurose, A., Tanaka, T., Traganos, F., Dai, W., and Darzynkiewicz, Z. Activation of ATM and histone H2AX phosphorylation induced by mitoxantrone but not by topotecan is prevented by the antioxidant N-acetyl-L-cysteine Cancer Biol. Ther. , 5: 959-964, 2006. 497. Mandecki, W., Ardelt, B., Corradetti, T., Davidovitz, H., Flint, J., Huang, Z., Kopacka, W., Lin, X., and Darzynkiewicz, Z. Microtransponders, the miniature radiofrequency-identifiable (RFID) electronic chips, as platforms for cell growth in cytotoxicity assays. Cytometry A, 69 A: 1097-1105, 2006. 498. Tanaka, T., Kurose, A., Halicka, H.D., Huang, X., Traganos, F., and Darzynkiewicz, Z. Nitrogen oxide-releasing aspirin induces histone H2AX phosphorylation, ATM activation, and apoptosis preferentially in S-phase cells; involvement of reactive oxygen species. Cell Cycle, 5: 1669-1674, 2006. 499. Tanaka, T., Halicka, H.D., Huang, X., Traganos, F., and Darzynkiewicz, Z. Constitutive histone H2AX phosphorylation and ATM activation, the reporters of DNA damage by endogenous oxidants. Cell Cycle, 5: 1940-1945, 2006. 500. Tanaka, T., Halicka, H.D., Traganos, F., and Darzynkiewicz, Z. Phosphorylation of histone H2AX on Ser 139 and activation of ATM during oxidative burst in phorbol ester-treated human leukocytes. Cell Cycle, 5: 2671 - 2675, 2006. 501. Huang, X., and Darzynkiewicz, Z. Cytometric assessment of histone H2AX phosphorylation, a reporter of DNA damage. Methods Molec. Biol. 314; 73-80, 2006. 502. Darzynkiewicz, Z., Huang, X., and Okafuji, M. Detection of DNA strand breaks by flow and lasers scanning cytometry in studies of apoptosis and cell proliferation (DNA Replication). Methods Molec. Biol. 314; 81-94, 2006. 503. Pozarowski, P., Holden, E., and Darzynkiewicz, Z. Laser scanning cytometry: Principles and applications. Methods Molec. Biol. 319: 165-192, 2006. 2007 504. Lin, J.H., Takano, T., Arcuino, G., Wang, X., Hu, F., Darzynkiewicz, Z., Nunes, M., Goldman, S.A., and Nedergaard, M., Purinergic signaling regulates neural progenitor cell expansion and neurogenesis. Dev. Biol., 302: 356-366, 2007 505. Tanaka, T., Kajstura, M., Halicka, H.D., Traganos, F., and Darzynkiewicz, Z. Constitutive histone H2AX phosphorylation and ATM activation are strongly amplified during mitogenic stimulation of lymphocytes. Cell Proliferation, 40: 1-13, 2007 506. Kajstura, M., Halicka, H.D., Pryjma, J. and Darzynkiewicz, Z. Discontinuous fragmentation of nuclear DNA during apoptosis revealed by discrete "sub-G1" peaks on DNA content histograms. Cytometry A, 71A: 125-131, 2007. 507. Tanaka, T., Halicka, H, D., Traganos, F., Seiter, K., and Darzynkiewicz, Z. Induction of ATM activation, histone H2AX phosphorylation and apoptosis by etoposide: relation to the cell cycle phase. Cell Cycle, 6: 371-376, 2007. 508. Halicka, H.D., Ardelt, B., Shogen, K., and Darzynkiewicz, Z. Mild hyperthermia predisposes tumor cells to undergo apoptosis upon treatment with onconase. Int. J. Oncol. 30: 841-847, 2007 509. Darzynkiewicz, Z., Pozarowski, P. All that glitters is not gold: All that binds FLICA is not caspase. A caution in data interpretation - and new opportunities.Cytometry A, 71A:, 36-37, 2007 510. Tanaka, T., Huang, X., Halicka, H.D., Zhao, H., Traganos, F., Albino, A.P., Dai, W., Darzynkiewicz, Z. Cytometry of ATM activation and histone H2AX phosphorylation to estimate extent of DNA damage induced by exogenous agents. Cytometry A , 71A, 648-661, 2007 511. Schulenburg, C., Ardelt, B, Ardelt, Arnold, U., Shogen, K., Ulbrich-Hofmann, R. Darzynkiewicz, Z. The interdependence between catalytic activity, conformational stability, and cytotoxicity of Onconase. Cancer Biol. Ther., 6: (8) Epub May 11, 2007 512. Tanaka, T., Huang, X., Jorgensen, E., Gietl, E., Traganos, F., Darzynkiewicz, Z., Albino, A.P. ATM activation accompanies histone H2AX phosphorylation in A549 cells upon exposure to tobacco smoke. BMC Cell Biology Epub June 26, 8:26, 2007. 513. Ardelt, B., Juan, G., Burfeind, P., Salomon, T., Wu, J.M., Hsieh, T-C., Li, X., Sperry, R., Pozarowski, P., Shogen, K., Ardelt, W., Darzynkiewicz, Z. Onconase, an anti-tumor ribonuclease suppresses oxidative stress. Int. J. Oncol., 31: 663-669, 2007. 514. Tadi, K., Ashok, B.T., Chen, Y., Banerjee, D., Wysocka-Skrzela, B., Konopa, J., Darzynkiewicz, Z., Tiwari, R.K. Pre-clinical evaluation of 1-nitroacridine derived chemotherapeutic agent that has preferential cyutotoxic activity towards prostate cancer. Cancer Biol. Ther. Jul 24, 2007 (Epub) 515. Zhao, H., Tanaka, T., Halicka, H.D., Traganos, F., Zarebski, M., Dobrucki, J., Darzynkiewicz, Z. Cytometric assessment of DNA damage by exogenous and endogenous oxidants reports the aging-related processes. Cytometry, A. 71A: 905-914, 2007. 516. Hsieh, T-C., Traganos, F., Darzynkiewicz, Z., Wu, J.M.The 2,6-disubstituted purine reversine induces growth arrest and polyploidy in human cancer cells. Int. J. Oncol. 31: 1293-1300, 2007. 517. Ardelt, B., Ardelt, W., Pozarowski, P., Kunicki, J., Shogen, K., Darzynkiewicz, Z. Cytostatic and cytotoxic properties of Amphinase, a novel cytotoxic ribonuclease from Rana pipiens oocytes. Cell Cycle, 6: 3097-3102; 2007. 2008 518. Smart, D.J., Halicka, H.D., Traganos, F., Darzynkiewicz, Z., Williams, G.M. Ciprofloxacin- induced G2 arrest and apoptosis in TK6 lymphoblastoid cells is not dependent on double-strand break formation. Cancer Biol. Ther. 7: 113-119, 2008. 519. Halicka, H.D., Ita, M., Tanaka T., Kurose, A., Darzynkiewicz, Z "The biscoclaurine alkaloid cepharanthine protects DNA in TK6 lymphoblastoid cells from constitutive oxidative damage. Pharmacological Reports, 60: 93-100, 2008. 520. Darzynkiewicz, Z. There's more than one way to skin a cat: Yet another way to assess mitotic index by cytometry. Cytometry A, 73A; 386-387, 2008. 521. Darzynkiewicz, Z., Galkowski, D., Zhao, H. Analysis of apoptosis by cytometry using TUNEL assay. Methods 44: 250-254, 2008. 522. Chen, S., Gao, J., Halicka, H.D., Traganos, F., and Darzynkiewicz, Z. Down-regulation of androgen-receptor and PSA by phytochemicals. Int. J. Oncol., 32: 405-411, 2008. 523. Wang, X., Yang, Y., Jiang, N., Duan, Q., Darzynkiewicz, Z., Dai, W. Sgo1 functions in centriole cohesion, which is partly regulated by Plk1. Dev Cell, 14: 331-341, 2008 524. Darzynkiewicz, Z. Broken beyond repair. Damaging DNA in glioblastoma cells with radiation and camptothecin. Cancer Biol. Ther. Epub March 2008 525. Wlodkowic, D., Skommer, J., Darzynkiewicz, Z. SYTO probes in cytometry of tumor cell death Cytometry Epub March 2008 526. Zhao, H., Tanaka, T., Mitlitski, V., Heeter, J., Balazs, E.A., Darzynkiewicz, Z. Protective effect of hyaluronate on oxidative DNA damage in WI-38 and A549 cells. Int J. Oncol., 32: 1159- 1169, 2008. 527. Ardelt, W., Shogen, K., Darzynkiewicz, Z. Onconase and amphinase, the anti-tumor ribonucleases from Rana pipiens oocytes. Curr. Pharm. Biotechnol. (in press) 528. Zhao, H., Traganos, F., Albino, A.P., Darzynkiewicz, Z. Oxidative stress induces cell cycle- dependent Mre11 recruitment, ATM and Chk2 activation and histone H2AX phosphorylation Cell Cycle, Epub. June 2008 529. Smart, D.J., Halicka, H.D., Schmuck, G., Traganos, F., Darzynkiewicz, Z., Williams, G.M Assessment of DNA double-strand breaks and H2AX induced by the topoisomerase II poisons etoposide and mitoxantrone. Mutation Res (in press) 530. Pietrzak, M., Halicka H. D. Wieczorek, Z., Wieczorek, J., Darzynkiewicz, Z. Attenuation of acridine mutagen ICR 191-DNA interactions and DNA damage by the mutagen interceptor chlorophyllin. Biophys. J. 135: 69-75. 531. Zhao, H., Traganos, F., Darzynkiewicz, Z. Kinetics of histone H2AX phosphorylation and Chk2 activation in A549 cells treated with topotecan and mitoxantrone in relation to the cell cycle phase. Cytometry A. Epub May 2008. 532. Wlodkowic, D., Skommer J., Hillier, C, Darzynkiewicz, Z. Multiparameter detection of apoptosis using red-excitable SYTO probes. Cytometry A, Epub May 2008. 533. Ita, M., Halicka, H.D., Tanaka, T., Kurose, A., Ardelt, B., Shogen, K., and Darzynkiewicz, Z. Remarkable enhancement of cytotoxicity of onconase and cepharanthine when used in combination on various tumor cell lines. Cancer Biol. & Therap., Epub Apr 19, 2008 534. Darzynkiewicz, Z., Halicka H.D. and Tanala T. Cytometric assessment of DNA damage induced by DNA topoisomerase inhihibitors. Meth. Molec. Biol. (in press) 535. Smolewski, P., Cebula, B., Jamroziak, K., Janus, A., Blagosklonny, M.V., Darzynkiewicz, Z., and Robak,, T. Rapamycin renders acute lymphoblastic leukemia cells susceptible to apoptosis induced by cladribine while protecting normal lymphocytes: a rationale for the "cyclotherapy" concept. Acta Haematol Polon (in press)
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