Zbigniew Darzynkiewicz, M.D., Ph.D.


 About Zbigniew 
  • Biography
  • Curriculum Vitae
  • Publications
  • Books
  • Chapters
  • Presentations
  • Research Accomplishments
  • US Patents
  • Most Cited Papers

     Essential Cytometry Methods 
  • Preface
  • Table of Contents

     Cytometry 4th Edition 
  • Preface
  • Table of Contents

      Most Cited (> 50 citations) Publications (Hirsch's citation h-index = 81):

    1. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808 (cited 1,581 times).

    2. Gorczyca W, Gong J, Darzynkiewicz. (1993) Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res 53:1945-1951 (cited 865 times).

    3. Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami, M. Traganos F. (1997) Cytometry in cell necrobiology. Analysis of apoptosis and accidental cell death (necrosis). Cytometry 27:1-20 (cited 748 times).

    4. Gong JP, Traganos F, Darzynkiewicz Z. (1994) A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal Biochem 218:314-319 (cited 563 times, GS).

    5. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1976) Lymphocyte stimulation: A rapid multiparameter analysis. Proc Natl Acad Sci USA 73:2881-2884 (cited 447 times)

    6. Gorczyca W, Gong JP, Ardelt B, Traganos F, Darzynkiewicz Z. (1993) The cell cycle related differences in susceptibility of HL-60 cells to apoptosis induced by various antitumor agents. Cancer Res 53:3186-3192 (cited 440 times).

    7. Traganos F, Darzynkiewicz Z, Sharpless T, Melamed MR. (1977) Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem 25:46-56 (cited 427 times).

    8. Evenson DP, Darzynkiewicz Z, Melamed MR. (1980) Relation of mammalian sperm chromatin heterogeneity to fertility. Science 210:1131-1133, 1980 (cited 419 times; GS).

    (8 “citation classics” – cited over 400 times each)


    9. Darzynkiewicz Z, Traganos F, Melamed MR.(1980) New cell cycle compartments identified by multiparameter flow cytometry. Cytometry 1:98-108 (cited 297 times).

    10. Gorczyca W, Bigman K, Mittelman A, Ahmed T, Gong J, Melamed MR, Darzynkiewicz Z. (1993) Induction of DNA strand breaks associated with apoptosis during treatment of leukemias. Leukemia (Baltimore) 7: 659-670 (cited 273 times).

    11. Darzynkiewicz Z. Li X, Gong JP. (1994) Assays of cell viability. Discrimination of cells dying by apoptosis. Method Cell Biol 41: 15-38 (cited 256 times).

    12. Bories D, Raynal M-C, Solomon DH, Darzynkiewicz Z, Cayre YE. (1989) Down-regulation of a serine protease, Myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells. Cell 59: 959-968 (cited 259 times).

    13. Darzynkiewicz Z, Sharpless T, Staiano-Coico L, Melamed MR. (1980) Subcompartments of the G1 phase of cell cycle detected by flow cytometry. Proc Natl Acad Sci USA 77:6696-6700 (cited 250 times).

    14. Jakóbisiak M, Bruno S, Skierski J, Darzynkiewicz Z. (1991) The cell cycle specific effects of lovastatin. Proc Natl Acad Sci USA 88:3628-3632 (cited 250 times).

    15. Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. (1993) Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells. Exp Cell Res 207:202-205 (cited 251 times).

    16. Andreeff M, Darzynkiewicz Z, Sharpless TK, Clarkson BD, Melamed MR. (1980) Discrimination of human leukemia subtypes by flow cytometric analysis of cellular DNA and RNA. Blood 55:282-293 (cited 249 times).

    17. Garcia-Valenzuela E, Gorczyca W, Darzynkiewicz Z, Sharma SC. (1994) Apoptosis in adult retinal ganglion cells after axotomy. J Neurobiol 25: 431-438 (cited 248 times).

    18. Gorczyca W, Bruno S, Darzynkiewicz RJ, Gong JP, Darzynkiewicz Z. (1992) DNA strand breaks occurring during apoptosis: Their early in situ detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors. Int J Oncol 1:639-648 (cited 233 times).

    19. Darzynkiewicz Z, Bedner E, Li X, Gorczyca W, Melamed MR.(1999) Laser scanning cytometry. A new instrumentation with many applications. Exp Cell Res 249:1-12 (cited 216 times).

    20. Darzynkiewicz Z, Williamson B, Carswell EA, Old LJ. (1984) The cell cycle specific effects of tumor necrosis factor. Cancer Res 44:83-90 (cited 215 times).

    21. Darzynkiewicz Z, Traganos F, Kapuscinski J, Staiano-Coico L, Melamed MR. (1984) Accessibility of DNA in situ to various fluorochromes: Relationship to chromatin changes during erythroid differentiation of Friend leukemia cells. Cytometry 5:355-363 (cited 212 times).

    22. Hsieh T-C, Juan G, Darzynkiewicz Z, Wu JM. (1999) Resveratrol increases nitric oxide synthase, induces accumulation of p53 and p21WAF1/CIP1 and suppresses cultured bovine pulmonary artery endothelial cell proliferation by perturbing progression through S and G2. Cancer Res 59: 2596-2601 (cited 205 times).

    23. Hotz MA, Gong JP, Traganos F, Darzynkiewicz Z. (1994) Flow cytometric detection of apoptosis. Comparison of the assays of in situ DNA degradation and chromatin changes. Cytometry 15:237-244 (cited 200 times).

    24. Darzynkiewicz Z, Traganos F, Sharpless TK, Melamed MR. (1975) Conformation of RNA in situ as studied by acridine orange staining and automated cytofluorometry. Exp Cell Res 95:143-153 (cited 197 times).

    25. Eisinger M, Lee JS, Hefton JM, Darzynkiewicz Z, Chiao KW, deHarven E. (1979) Human epidermal cell cultures: Growth and differentiation in the absence of dermal components or medium supplements. Proc Natl Acad Sci USA 76:5340-5345 (cited 198 times).

    26. Olszewski W, Darzynkiewicz Z, Rosen P, Schwartz MK, Melamed MR. (1981) Flow cytometry of breast carcinoma. I. Relation of DNA ploidy level to histology and estrogen receptors. Cancer 48:980-984 (cited 192 times).

    27. Del Bino G, Lassota P, Darzynkiewicz Z. (1991) The S-phase cytotoxicity of camptothecin. Exp Cell Res 193:27-35 (cited 190 times).

    28. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1975) Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry. Exp Cell Res 90:411-428 (cited 182 times).

    29. Bruno S, Darzynkiewicz Z. (1992) Cell cycle dependent expression and stability of the nuclear protein detected by Ki-67 antibody in HL-60 cells. Cell Prolif 25:31-40 (cited 181 times).

    30. Bedner E, Li X, Gorczyca W, Melamed MR, Darzynkiewicz Z. (1999) Analysis of apoptosis by laser scanning cytometry. Cytometry 35: 181-195 (cited 178 times).

    31. Darzynkiewicz Z, Traganos F, Staiano-Coico L., Kapuscinski J, Melamed MR. (1982) Interactions of rhodamine 123 with living cells studied by flow cytometry. Cancer Res 42:799-806 (cited 172 times).

    32. Darzynkiewicz Z, Staiano-Coico L, Melamed MR. (1981) Increased mitochondrial uptake of rhodamine 123 during lymphocyte stimulation. Proc Natl Acad Sci USA 78:2383-2387 (cited 167 times).

    33. Traganos F, Ardelt B, Halko NM, Bruno S, Darzynkiewicz Z. (1992) Effect of Genistein, a tyrosine kinase inhibitor, on the growth and cell cycle progression of normal human lymphocytes and human leukemic MOLT-4 and HL-60 cells. Cancer Res 52:6200-6208 (cited 168 times).

    34. Kapuscinski J, Darzynkiewicz Z, Traganos F, Melamed MR. (1981) Interactions of a new antitumor agent 1,4-dihydroxy-5-8-bis 2-(hydroxyethyl)amino)ethyl)amino)--9,10-anthracenedione with nucleic acids. Biochem Pharmacol 30:231-240 (cited 157 times).

    35. Darzynkiewicz Z, Gong JP, Juan G, Ardelt B, Traganos, F. (1996) Cytometry of cyclin proteins. Cytometry 25:1-13, 1996 (cited 154 times).

    36. Li X, Darzynkiewicz Z. (1995) Labeling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation. Cell Proliferation 28: 571-579 (cited 148 times).

    37. Sharpless T, Traganos F, Darzynkiewicz Z, Melamed MR. (1975) Flow cytofluorometry: Discrimination between single cells and cell aggregates by direct size measurements. Acta Cytol 19:577-581 (cited 145 times).

    38. Del Bino G, Darzynkiewicz Z. (1991) Camptothecin, teniposide or m-AMSA but not mitoxantrone or doxorubicin induce degradation of nuclear DNA in S-phase of HL-60 cells. Cancer Res 51:1165-1169 (cited 143 times).

    39. Venturelli D, Martinez R, Melotti P, Casella I, Peschle C, Cucco C, Spaminato G, Darzynkiewicz Z, Calabretta B. (1995) Overexpression of DR-nm23, a protein encoded by a member of nm23 family, inhibits granulocyte differentiation and induces apoptosis in 32Dc13 myeloid cells. Proc Natl Acad Sci USA 92: 7435-7439 (cited 142 times).

    40. Evenson D, Darzynkiewicz Z, Jest L, Janca F, Ballachey B. (1986) Changes in accessibility of DNA in situ to various fluorochromes during spermatogenesis. Cytometry 7:45-53 (cited 138 times).

    41. Crissman HA, Darzynkiewicz Z, Tobey RA, Steinkamp JA. (1985) Correlated measurements of DNA, RNA and protein content in individual cells by flow cytometry. Science 228:1321-1324 (cited 136 times).

    42. Darzynkiewicz Z, Evenson DP, Staiano-Coico L, Sharpless T, Melamed MR. (1979) Correlation between cell cycle duration and RNA content. J Cell Physiol 100:425-438 (cited 135 times).

    43. Blagosklonny MV, Robey R, Sackett DL, Du L, Traganos F, Darzynkiewicz Z, Fojo T, Bates SE. (2002) Histone deacetylase inhibitors all induce p21 but differentially cause tubulin acetylation, mitotic arrest, and cytotoxicity. Mol Cancer Ther 1: 937-941 (cited 135 times; GS)

    44. Evenson DP, Darzynkiewicz Z, Melamed MR. (1982) Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility. J Histochem Cytochem 30:279-280 (cited 135 times).

    45. Juan G, Traganos F, James WM, Ray JM, Roberge M, Sauve DM, Anderson H, Darzynkiewicz Z. (1998) Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis. Cytometry 32: 71-77 (cited 134 times).

    46. Bedner E, Smolewski P, Amstad P, Darzynkiewicz Z. (2000) Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp Cell Res 259: 308-313 (cited 132 times).

    47. Darzynkiewicz Z. (1979) Acridine orange as a molecular probe in studies of nucleic acids in situ. In: Flow Cytometry and Sorting. Melamed MR, Mendelsohn M & Mullaney P. (eds.), John Wiley and Sons, New York, pp. 285-316 (cited 129 times).

    48. Darzynkiewicz Z, Evenson DP, Staiano-Coico L, Sharpless T, Melamed MR. (1979) Relationship between RNA content and progression of lymphocytes through the S phase of the cell cycle. Proc Natl Acad Sci USA 76:358-362 (cited 128 times).

    49. Li X, Traganos F, Melamed MR, Darzynkiewicz Z. (1995) Single step procedure for DNA strand-breaks labeling. Detection of apoptosis and DNA replication. Cytometry 20: 172-180 (cited 126 times).

    50. Darzynkiewicz Z, Crissman HA, Traganos F, Steinkamp J. (1982) Cell heterogeneity during the cell cycle. J. Cell Physiol 113:465-474 (cited 124 times).

    51. Darzynkiewicz Z, Carter SP, Mikulski SM, Shogen K. (1988) Cytostatic and cytotoxic effects of Pannon, a novel anticancer agent. Cell Tissue Kinet 21:169-182 (cited 120 times).

    52. Traganos F, Evenson DP, Staiano-Coico L, Darzynkiewicz Z, Melamed MR. (1980) The action of dihydroxyanthraquinone on cell cycle progression and survival of a variety of cultured mammalian cells. Cancer Res 40:671-681 (cited 118 times).

    53. Bruno S, Ardelt B, Skierski JS, Traganos F, Darzynkiewicz Z. (1992) Different effects of staurosporine, an inhibitor of protein kinases, on the cell cycle and chromatin structure of normal and leukemic lymphocytes. Cancer Res 52:470-473 (cited 118 times)

    54. Moran R, Darzynkiewicz Z, Staiano-Coico L,Melamed MR. (1985) Detection of BrdUrd incorporation by monoclonal antibodies: Role of the DNA denaturation step. J Histochem Cytochem 33: 821-827 (cited 116 times).

    55. Darzynkiewicz Z. (1995) Apoptosis in antitumor strategies: Modulation of cell cycle or differentiation. J Cell Biochem 58:51-159 (cited 117 times).

    56. Gong JP, Ardelt B, Traganos F, Darzynkiewicz Z. (1994) Unscheduled expression of cyclin B1 and cyclin E in several leukemic and solid tumor cell lines. Cancer Res 54:4285-4288 (cited 115 times)

    57. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1977) Cell cycle related changes in nuclear chromatin of stimulated lymphocytes as measured by flow cytometry. Cancer Res 37:4635-4640 (cited 110 times).

    58. Melamed MR, Enker WE, Janov AJ, Kessler G, Darzynkiewicz Z. (1986) Flow cytometry of colorectal carcinoma with three years follow-up. Dis Col Rec 29:184-186 (cited 105 times).

    59. Del Bino G, Skierski JS, Darzynkiewicz Z. (1990) Diverse effects of camptothecin, an inhibitor of topoisomerase I on the cell cycle of lymphocytic (L1210, MOLT-4) and myelogenous (HL-60, KG1) leukemic cells. Cancer Res 50: 5746-5750 (cited 104 times).

    60. Bruno S, Del Bino G, Lassota P, Giaretti W, Darzynkiewicz Z. (1992) Inhibitors of proteases prevent endonucleolysis accompanying apoptotic cell death. Leukemia 6:1113-1120 (cited 102 times).

    61. Collste LG, Devonec M, Darzynkiewicz Z, Traganos F, Sharpless TK, Whitmore WF, Jr, Melamed MR. (1980) Bladder cancer diagnosis by flow cytometry. Correlation between cell samples from biopsy and bladder irrigation fluid. Cancer 45: 2389-2394 (cited 101 times).

    62. Olszewski W, Darzynkiewicz Z, Rosen P, Schwartz MK, Melamed MR. (1981) Flow cytometry of breast carcinoma. II. Relation of tumor cell cycle distribution to histology and estrogen receptor. Cancer 48:985-988 (cited 101 times).

    63. Gong JP, Traganos F, Darzynkiewicz Z. (1995) Growth imbalance and altered expression of cyclins B1, A. E and D3 in MOLT-4 cells synchronized in the cell cycle by inhibitors of DNA replication. Cell Growth & Differentiation 6: 1485-1493 (cited 101 times)

    64. Gong JP, Li X, Darzynkiewicz Z. (1993) Different patterns of apoptosis of HL-60 cells induced by cycloheximide and camptothecin. J Cell Physiol 157: 263-270 (cited 100 times).


    65. Balazs EA, Darzynkiewicz Z. (1973) The effect of hyaluronic acid on fibroblasts, mononuclear phagocytes and lymphocytes. In: Biology of the Fibroblast, Papers of the Symposium held in Turku, Finland, 1972; Kulonen, E., and Pikkarainen, J., (Eds.), Academic Press, London, pp. 237-252 (cited 98 times).

    66. Huang X, Okafuji M, Traganos F, Luther E, Holden E, Darzynkiewicz Z. (2004) Assessment of histone H2AX phosphorylation induced by DNA topoisomerase I and II inhibitors topotecan and mitoxantrone and by DNA crosslinking agent cisplatin. Cytometry A, 58A: 99-110 (cited 97 times)

    67. Del Bino G, Skierski J, Darzynkiewicz Z. (1991) The concentration-dependent diversity of effects of DNA topoisomerase I and II inhibitors on the cell cycle of HL-60 cells. Exp Cell Res 195:485-491 (cited 97 times).

    68. Bruno S, Lassota P, Giaretti W, Darzynkiewicz Z. (1992) Apoptosis of rat thymocytes triggered by prednisolone, camptothecin, or teniposide is selective to G0 cells and is prevented by inhibitors of proteases. Oncol Res 4:29-35 (cited 97 times).

    69. Kapuscinski J, Darzynkiewicz Z, Melamed MR. (1982) Luminescence of the solid complexes of acridine orange with RNA. Cytometry 2:201-211 (cited 96 times).

    70. Darzynkiewicz Z, Traganos F, Andreeff M, Sharpless TK, Melamed MR. (1979) Different sensitivity of chromatin to acid denaturation in quiescent and cycling cells as revealed by flow cytometry. J Histochem Cytochem 27:478-485 (cited 94 times).

    71. Hsieh T-C, Burfeind P, Laud K, Backer JM, Traganos F, Darzynkiewicz Z, Wu JM. (1999) Cell cycle effects and control of gene expression by resveratrol in breast carcinoma cell lines with different metastatic potential. Int J Oncol 15: 245-252 (cited 93 times)

    72. Wang ZQ, Dudhane A, Orlikowsky T, Clarke K, Li X, Darzynkiewicz Z. Hoffman, M.K. (1994) CD4 engagement in vivo induces apoptosis in T cells. Eur J Immunol 24: 1549-1552 (cited 93 times).

    73. Hotz MA, Traganos F, Darzynkiewicz Z. (1992) Changes in nuclear chromatin related to apoptosis or necrosis induced by the DNA topoisomerase II inhibitor fostriecin in MOLT-4 and HL-60 cells are revealed by altered DNA sensitivity to denaturation. Exp Cell Res 201:184-191 (cited 93 times).

    74. Li X, Gong J, Feldman E, Seiter K, Traganos F, Darzynkiewicz Z. (1994) Apoptotic cell death during treatment of leukemias. Leukemia & Lymphoma 13: Suppl 1, 65-70 (cited 92 times).

    75. Kajstura J, Zhang X, Reiss K, Szoke E, Li P, Lagrasta C, Cheng W, Darzynkiewicz Z, Olivetti G, Anversa P. (1994) Myocyte cellular hyperplasia and myocyte cellular hypertrophy contribute to chronic ventricular remodeling in coronary artery narrowing induced cardiomyopathy in rats. Circ Res 74: 383-400 (cited 92 times).

    76. Halicka HD, Bedner E, Darzynkiewicz Z. (2000) Segregation of RNA and separate packaging of DNA and RNA in apoptotic bodies during apoptosis. Exp Cell Res, 260:248-255 (cited 92 times).

    77. Devonec M, Darzynkiewicz Z, Kostyrka-Claps ML, Collste L, Whitmore WF, Melamed MR. (1982) Flow cytometry of low stage bladder tumors: Correlation with cytologic and cytoscopic diagnosis. Cancer 49:109-118 (cited 91 times).

    78. Darzynkiewicz Z, Kapuscinski J. (1990) Acridine orange, a versatile probe of nucleic acids and other cell constituents. In: Flow Cytometry and Sorting. Melamed MR, Mendelsohn M & Lindmo T, (eds.), Alan R. Liss, Inc., New York. pp. 291-314 (cited 90 times).

    79. Darzynkiewicz Z. (1990) Differential staining of DNA and RNA in intact cells and isolated cell nuclei with acridine orange. Method Cell Biol 33: 285-298 (cited 89 times).

    80. Del Bino G, Bruno S, Yi PN, Darzynkiewicz Z. (1992) Apoptotic cell death triggered by camptothecin or teniposide: The cell cycle specificity and effects of ionizing radiation. Cell Proliferation 25:537-548 (cited 88 times).

    81. Collste LG, Darzynkiewicz Z, Traganos F, Sharpless T, Sogani P, Grabstald H, Whitmore WF, Jr. Melamed MR. Flow cytometry in cancer detection and evaluation using acridine orange metachromatic nucleic acid staining of irrigation cytology specimens. J Urol 1980; 123:478-485 (cited 88 times).

    82. Kapuscinski J, Darzynkiewicz Z. (1986) Relationship between the pharmacological activity of antitumor drugs Ametantrone and Mitoxantrone (Novantrone) and their ability to condense nucleic acids. Proc Natl Acad Sci USA 83: 6302-6306 (cited 87 times).

    83. Darzynkiewicz Z., Bedner, E., Smolewski, P. (2001) Flow cytometry in analysis of cell cycle and apoptosis. Semin Hematol 38: 179-193 (cited 88 times)

    84. Traganos F, Kapuscinski J, Darzynkiewicz Z. (1991) Caffeine modulates the effects of DNA intercalating drugs in vitro: A flow cytometric and spectrophotometric analysis of caffeine interaction with Novantrone, doxorubicin, ellipticine and the doxorubicin analog, AD198. Cancer Res 51:3682-3689 (cited 86 times)

    85. Huang X, Halicka HD, Traganos F, Tanaka T, Kurose A, Darzynkiewicz Z. (2005) Cytometric assessment of DNA damage in relation to cell cycle phase and apoptosis. Cell Prolif 38: 223-243 (cited 85 times)

    86. Abraham NG, Kushida T, McClung J, Weiss M, Quan S, Lafaro R, Darzynkiewicz Z, Wolin M. (2003) Heme oxygenase-1 attenuates glucose-mediated cell growth arrest and apoptosis in human microvessel endothelial cells. Circ Res., 93:507-514 (cited 85 times).

    87. Kapuscinski J, Darzynkiewicz Z. (1985) Interactions of antitumor agents ametantrone and mitoxantrone (novantrone) with double-stranded DNA. Biochem Pharmacol 34:4203-4213 (cited 84 times).

    88. Gorczyca W, Tuziak T, Kram A, Melamed MR, Darzynkiewicz Z. (1994) Detection of apoptosis in fine-needle aspiration biopsies by in situ end-labeling of fragmented DNA. Cytometry 15: 169-175 (cited 83 times).

    89. Staiano-Coico L, Darzynkiewicz Z, Melamed MR, Weksler M. (1984) Immunological studies of aging. Impaired proliferation of T lymphocytes in elderly humans by flow cytometry. J Immunol 132:1788-1792 (cited 82 times).

    90. Li X, Melamed MR, Darzynkiewicz Z. (1996) Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color. Exp Cell Res 222: 28-37 (cited 82 times)

    91. Tanaka T, Halicka HD, Huang X, Traganos F, Darzynkiewicz Z. (2006) Constitutive histone H2AX phosphorylation and ATM activation, the reporters of DNA damage by endogenous oxidants. Cell Cycle 5:1940-1945 (cited 81 times).

    92. Smolewski P, Bedner E, Du L, Hsieh TC, Wu JM, Phelps DJ, Darzynkiewicz Z. (2001) Detection of caspases activation by fluorochrome-labeled inhibitors: multiparameter analysis by laser scanning cytometry, Cytometry, 44: 73-82 (cited 80 times).

    93. Zhang JC, Claffey K, Sakthivel R, Darzynkiewicz Z, Shaw DE, Leal J, Wang YC, Lu FM, MacCrae KR. (2000) Two-chain high molecular weight kininogen induces endothelial cell apoptosis and inhibits angiogenesis: partial activity with domain 5. FASEB J 14: 2589-2600 (cited 80 times)

    94. Halicka HD, Huang X, Traganos F, King MA, Dai W, Darzynkiewicz Z. (2005) Histone H2AX phosphorylation after cell irradiation with UV-B: Relationship to cell cycle phase and induction of apoptosis. Cell Cycle 4:339-345 (cited 77 times)

    95. Wang Q, Liu TY, Fang YQ, Xie SQ, Huang X, Mahmood R, Ramaswamy G, Sakamoto KM, Darzynkiewicz Z, Xu M, Dai W. (2004) BUBR1 deficiency results in abnormal megakaryopoiesis. Blood 103: 1278-85 (cited 79 times)

    96. Li, X., Darzynkiewicz, Z. (2000) Cleavage of poly(ADP-ribose)polymerase measured in situ in individual cells: relationship to DNA fragmentation and cell cycle position during apoptosis. Exp Cell Res 255: 125-132 (cited 78 times).

    97. Huang X, Traganos F, Darzynkiewicz Z. (2003) DNA damage induced by DNA topoisomerase I- and topoisomerase II- inhibitors detected by histone H2AX phosphorylation in relation to the cell cycle phase and apoptosis. Cell Cycle 2:614-619 (cited 76 times).

    98. Darzynkiewicz Z, Gledhill BL, Ringertz NR. (1969) Changes in deoxyribonucleoprotein during spermiogenesis in the bull. 3H-actinomycin D binding. Exp Cell Res 58: 435-438 (cited 76 times).

    99. Deptala, A., Bedner, E., Gorczyca, W., and Darzynkiewicz, Z. (1998) Activation of nuclear factor kappa B (NF-?B) by laser scanning cytometry (LSC). Cytometry, 33: 376-382 (cited 76 times)

    100. Pozarowski P, Huang X, Halicka DH, Lee B, Johnson G, Darzynkiewicz Z. (2003) Interactions of fluorochrome-labeled caspase inhibitors with apoptotic cells. A caution in data interpretation. Cytometry A, 55A: 50-60 (cited 76 times).

    101. Darzynkiewicz Z, Traganos F, Xue SB, Melamed MR. (1981) Effect of n-butyrate on cell cycle progression and in situ chromatin structure of L1210 cells. Exp Cell Res 136:279-293 (cited 74 times)

    102. Kajstura J, Pertoldi B, Leri A, Beltrami C-A, Deptala A, Darzynkiewicz Z, Anversa P. (2000) Telomere shortening: an in vivo marker of myocyte replication and aging. Am J Pathol 156:813-819 (cited 73 times)

    103. Darzynkiewicz Z, Balazs EA. (1971) Effect of connective tissue intercellular matrix on lymphocyte stimulation. I. Suppression of lymphocyte stimulation by hyaluronic acid. Exp Cell Res 66:113-123 (cited 71 times).

    104. Tanaka T, Huang X, Halicka HD, Zhao H, Traganos F, Albino AP, Dai W, Darzynkiewicz Z. (2007) Cytometry of ATM activation and histone H2AX phosphorylation to estimate extent of DNA damage induced by exogenous agents. Cytometry A 71A:648-661 (cited 71 times).

    105. Albino AP, Juan G, Traganos F, Reinart L, Connolly J, Rose DP, Darzynkiewicz Z. (2000) G1 arrest of melanoma cells by docosahexaenoic acid (omega-3 fatty acid) is associated with decreased pRB phosphorylation. Cancer Res 60:4130-4145 (cited 72 times).

    106. Wang Q, Xie S, Chen J, Fukasawa K, Naik U, Traganos F, Darzynkiewicz Z, Jhanwar-Uniyal M, Dai W. (2002) Cell cycle arrest and apoptosis induced by human polo-like kinase 3 is mediated through perturbation of microtubule integrity. Mol. Cell Biol. 22: 3450-3459 (cited 71 times)

    107. Staiano-Coico L, Darzynkiewicz Z, Hefton JM, Dutkowski R, Darlington GJ, Weksler M. (1983) Increased sensitivity of lymphocytes from old humans to cell cycle arrest and chromosomal damage induced by 3H-TdR. Science 219:1335-1337 (cited 70 times)

    108. Del Bino G, Darzynkiewicz Z, Degraef C, Mosselmans R, Galand P. (1999) Comparison of methods based on annexin V binding, DNA content or TUNEL for evaluating cell death in HL-60 and adherent MCF-7 cells. Cell Prolif 32: 25-37 (cited 70 times)

    109. Evenson DP, Darzynkiewicz Z, Melamed MR. (1980) Comparison of human and mouse sperm chromatin by flow cytometry. Chromosoma 78:225-238 (cited 69 times)

    110. Ringertz NR, Gledhill BL, Darzynkiewicz Z. Changes in deoxyri-bonucleoprotein during spermiogenesis in the bull. II. Sensitivity of DNA to heat denaturation. Exp Cell Res 1970; 62:204-218. (cited 69 times)

    111. Darzynkiewicz Z, Ringertz NR, Bolund L. (1969) Actinomycin D binding properties of stimulated human lymphocytes. Exp Cell Res 56:411-417 (cited 69 times)

    112. Darzynkiewicz Z, Traganos F. Measurement of apoptosis. (1998) In: Advances in Biochemical Engineering/Biotechnology. Apoptosis. M. Al-Rubeai (Ed). pp 33-73 (cited 64 times)

    113. Juan, G., Ardelt, B., Mikulski, S.M., Shogen, K,M Ardelt, W., Mittelman, A., Darzynkiewicz, Z. (1998) G1 arrest of U-937 cells by onconase is associated with suppression of cyclin D3 expression, induction of p16INK4A, p21WAF1/CIP1 and p27KIP and decreased pRb phosphorylation. Leukemia, 12:1241-1248 (cited 63 times)

    114. Fu C, Hsieh T-C, Guo J, Kunicki J, Lee MYWT, Darzynkiewicz Z, Wu JM. (2004) Licochalcone-A, a novel flavonoid, causes G2 and late-G1 arrests in PC-3 cells. Biochem Biphys Res Commun 322:263-270 (cited 64 times).

    115. Seiter K, Feldman E J, Halicka D, Traganos F, Darzynkiewicz Z, Lake D, Ahmed T. (1997) Phase I clinical and laboratory evaluation of topotecan and cytoarabine in patients with acute leukemia. J Clin Oncol 15: 44-55 (cited 61 times)

    116. Leri A, Berlucchi I, Limana F, Deptala A, Darzynkiewicz Z, Hintze TH, Kajstura J, Nadal-Ginard B, Anversa P. (2001) Telomerase expression and activity are coupled with myocyte proliferation and preservation of telomeric length in the failing heart. Proc Natl Acad Sci USA 98:8626-8631 (cited 61 times).

    117. Darzynkiewicz Z, Bolund L, Ringertz NR.(1969) Actinomycin D binding of normal and phytohemagglutinin stimulated lymphocytes. Exp Cell Res 55:120-126 (cited 61 times)

    118. Capasso JM, Bruno S, Cheng W, Li P, Rodgers R, Darzynkiewicz Z, Anversa P. (1992) Ventricular loading is coupled with DNA synthesis in adult cardiac myocytes after acute and chronic myocardial infarction in rats. Circulation Res 71: 1379-1389 (cited 60 times)

    119. Darzynkiewicz Z, Bedner E, Traganos F, Murakami T. (1998) Critical aspects in analysis of apoptosis and necrosis. Human Cell 11:3-12 (cited 60 times).

    120. Darzynkiewicz Z, Traganos F, Xue S, Staiano-Coico L, Melamed MR. (1981) Rapid analysis of drug effects on the cell cycle. Cytometry 1:279-286 (cited 59 times)

    121 Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1977) Recognition of cells in mitosis by flow cytofluorometry. J. Histochem Cytochem 25:875-880 (cited 58 times)

    122 Vibhakar R, Juan G, Traganos F, Darzynkiewicz Z. (1997) Finger L. Activation induced expression of human programmed death-1 gene in T-lymphocytes. Exp Cell Res 232: 25-28 (cited 57 times).

    123 Kapuscinski J, Darzynkiewicz Z. (1984) Condensation of nucleic acids by intercalating aromatic cations. Proc Natl Acad Sci USA 81:7368-7372 (cited 57 times)

    124 Koyama M, Takahashi K, Chou T-C, Darzynkiewicz Z, Kapuscinski J, Kelly TR, Watanabe KA. (1989) Intercalating agents with covalent bond forming capability. Novel type of potential anticancer agents. 2. Derivatives of chrysophanol and emodin. J Med Chem 32: 1594-1599 (cited 57 times).

    125 Rivas CI, Wisniewski D, Strife A, Perez A, Lambek C, Bruno S, Darzynkiewicz Z, Clarkson B. (1992) Constitutive expression of p53 protein in enriched normal human marrow blast cell populations. Blood 79:1982-1986 (cited 54 times)

    126 Halicka DH, Ardelt B, Li X, Melamed MR, Darzynkiewicz Z. (1995) 2-deoxyglucose enhances sensitivity of human histiocytic U 935 cells to apoptosis induced by Tumor Necrosis Factor. Cancer Res., 55:444-449 (cited 54 times).

    127. Smolewski P, Grabarek J, Halicka HD, Darzynkiewicz Z. Assay of caspases activation in situ combined with probing plasma membrane integrity detects three distinct stages of apoptosis. J Immunol Meth 2002; 265:111-121 (cited 53 times).

    128 Murakami T, Li X, Gong JP, Bhatia U, Traganos F, Darzynkiewicz Z. (1995) Induction of apoptosis by 5-azacytidine. Drug concentration dependent differences in cell cycle specificity. Cancer Res 1995; 55:3093-3098 (cited 52 times)

    129 Gong JP, Li X, Traganos F, Darzynkiewicz Z. (1994) Expression of G1 and G2 cyclins measured in individual cells by multiparameter flow cytometry; a new tool in the analysis of the cell cycle. Cell Prolif 27: 357-371 (cited 52 times)

    130 Kapuscinski J, Darzynkiewicz Z, Melamed MR. Interactions of acridine orange with nucleic acids. Properties of complexes of acridine orange with single stranded RNA. Biochem Pharmacol 1983; 32:3679-3694 (cited 52 times).

    131. Ronai Z, Tillotson JK, Traganos F, Darzynkiewicz Z, Conaway C, Upadhyaya P, El-Bayoumy K.(1995) Effects of organic and inorganic selenium compounds on rat mammary tumor cells. Int J Cancer 63: 428-434 (cited 52 times).

    132. Darzynkiewicz Z (1994) Simultaneous analysis of cellular RNA and DNA content. Method Cell Biol. 1994, 41: 401-420 (cited 53 times).

    133. Halicka HD, Ardelt B, Juan G, Mittelman A, Chen S, Traganos F, Darzynkiewicz Z. (1997). Apoptosis and cell cycle effects induced by extracts of the Chinese herbal preparation PC SPES. Int J Oncol 11:437-448 (cited 52 times).

    134. Chen S, Ruan Q, Bedner E, Deptala A, Wang X, Hsieh T-C, Traganos F, Darzynkiewicz Z. Effects of the flavonoid baicalin and its metabolite baicalein on androgen receptor expression, cell cycle progression and apoptosis of prostate cancer cell lines. Cell Prolif 2001; 34: 293-304 (cited 53 times)

    135. Del Bino G, Li X, Traganos F, Darzynkiewicz Z. (1994) Altered susceptibility of differentiating HL-60 cells to apoptosis induced by antitumor drugs. Leukemia 6: 281-288 (cited 52 times)

    136. Traganos F, Darzynkiewicz Z. (1994) Lysosomal proton pump activity: supravital cell staining with acridine orange differentiates leukocyte subpopulations.Methods Cell Biol. 1994, 41:185-194 (cited 52 times).

    137. Andreeff M, Beck JD, Darzynkiewicz Z, Traganos F, Gupta S, Melamed MR, Good RA. RNA content in human lymphocyte subpopulations. Proc Natl Acad Sci USA 1978; 75:1938-1942 (cited 51 times)

    138. Traganos F, Kapuscinski J, Gong JP, Ardelt B, Darzynkiewicz RJ, Darzynkiewicz Z. (1993) Caffeine prevents apoptosis and cell cycle effects induced by camptothecin or topotecan in HL-60 cells. Cancer Res 1993; 53: 4613-4618 (cited 51 times)

    139. Kapuscinski J, Darzynkiewicz Z. Interactions of acridine orange with double stranded nucleic acids. Spectral and affinity studies. J Biomol Struct Dyn 1987; 5:127-142. (cited 51 times)

    140. Darzynkiewicz Z., Barnard EA. (1967) Specific proteases of the rat mast cell. Nature 213:1198-1202 (cited 50 times)

    141. Darzynkiewicz Z, Andreeff M, Traganos F, Sharpless T, Melamed MR. (1978) Discrimination of cycling and noncycling lymphocytes by BrdUrd-suppressed acridine orange fluorescence in a flow cytometric system. Exp Cell Res 115:31-35 (cited 50 times)

    142. Darzynkiewicz Z. “Probing Nuclear Chromatin by Flow Cytometry.” Chapter in: Flow Cytometry and Sorting. Melamed MR, Mendelsohn M & Lindmo T (eds), Alan R Liss, Inc., New York. 1990, pp. 315-340 (cited 50 times)

    143. Hotz MA, Del Bino G, Lassota P, Traganos F, Darzynkiewicz Z. Cytostatic and cytotoxic effects of the DNA topoisomerase II inhibitor, fostriecin, on human promyelocytic HL-60 and lymphocytic MOLT-4 leukemic cells. Cancer Res 1992; 52:1530-1535 (cited 50 times).

    144. Darzynkiewicz Z, Traganos F, Chen S. Chinese herbal mixture PC-SPES in treatment of prostate cancer. A review. Int J Oncol 2000; 17:729-736. (cited 50 times)

    Contact: Zbigniew Darzynkiewicz